Caspase 9 multiplex activity assay kit fluorometric

Caspase activity assays in multi-well plate formats represent powerful tools for understanding experimental modulation of the apoptotic response. Caspase 3, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915) are purchased by Abcam. It a fluorescent assay that detects the activity of caspase-3/-8/-9 in cell lysates. We followed the protocol in https://www.abcam.com/ps/products/219/ab219915/documents/. Subtract blank readings from all measurements (control and treated)—using fluorescent intensity, determine fold change between control and treated cells. The fluorescence intensity was measured with FlexStation fluorescence microplate reader at the indicated wavelength. Caspase 3 (Ex/Em = 535/620 nm), Caspase-8 (Ex/Em = 490/525 nm), and caspase-9 (Ex/Em = 370/450 nm), activities can be detected in a single assay without interferences from other caspases.

Caspase activity assays in multi-well plate formats represent powerful tools for understanding experimental modulation of the apoptotic response. Caspase 3, Caspase 8, and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915) were purchased by Abcam. It a fluorescent assay that detects the activity of caspase 3/ 8/ 9 in cell lysates. We followed the protocol in https://www.abcam.com/ps/products/219/ab219915/documents/. Subtracting blank readings from all measurements (control and treated), using fluorescent intensity, determines fold change between control and treated cells. The fluorescence intensity was measured with FlexStation fluorescence microplate reader at the indicated wavelength. Caspase 3 (Ex/Em = 535/620 nm), Caspase-8 (Ex/Em = 490/525 nm), and caspase-9 (Ex/Em = 370/450 nm), activities can be detected in a single assay without interferences from other caspases.