Tsq vantage bench top mass spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The TSQ Vantage is a bench-top mass spectrometer designed for high-performance quantitative and qualitative analysis. It features a triple quadrupole configuration and is capable of performing a variety of mass spectrometry techniques, including selected reaction monitoring (SRM), multiple reaction monitoring (MRM), and full-scan analysis.

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Lab products found in correlation

Milliplex map human high sensitivity t cell magnetic bead panel immunoassay, by Merck Group (1 mentions) Cobas c system, by Hitachi (1 mentions) Spd 10a uv detector, by Shimadzu (1 mentions) Lc 10avp system, by Shimadzu (1 mentions) Accela uplc pump, by Thermo Fisher Scientific (1 mentions)

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TSQ Vantage (98 citations) TSQ Vantage mass spectrometer (25 citations)

2 protocols using tsq vantage bench top mass spectrometer

Biomarker Quantification in Plasma

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The measurement of plasma urea and calculation of eGFR were performed by the Sydney Adventist Hospital pathology laboratory using methods well established for clinical laboratories. Briefly, fasting plasma urea levels were determined by the enzymatic method on a Roche/Hitachi cobas c system. Estimated Glomerular filtration rate (eGFR) was calculated using the simplified Modification of Diet in Renal Disease study equation: GFR (ml/min/1.73m²) = 186 × (serum creatinine level [mg/dl])-1.154 × (age)-0.203× [0.742, if female] × [1.212, if black] [23 (link), 24 ].
Plasma levels of NAD⁺ and its metabolites [NADP⁺, cyclic ADP ribose (cADPR), nicotinamide (NAM), N-methylnicotinamide (MeNAM)] were measured by liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS), as previously described [25 ]. LC/MS/MS was carried out using a UPLC-MSD assembly consisting of an Accela UPLC pump, Accela AS injector, and a TSQ Vantage bench-top mass spectrometer (ThermoFisher Scientific, Waltham, US).
Plasma interleukin-6 (IL-6) levels were quantitated using the MILLIPLEX® MAP Human High Sensitivity T Cell Magnetic Bead Panel immunoassay (Merck KGaA, Darmstadt, Germany).
Plasma Kynurenine (Kyn) and Tryptophan (Trp) levels were measured by high-performance liquid chromatography (Shimadzu LC-10AVP system, equipped with SPD- 10A UV detector, Kyoto, Japan), as previously described [26 (link)].

Seyedsadjadi N., Berg J., Bilgin A.A., Braidy N., Salonikas C, & Grant R. (2018). High protein intake is associated with low plasma NAD+ levels in a healthy human cohort. PLoS ONE, 13(8), e0201968.

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Quantitative Metabolic Profiling by LC/MS/MS

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LC/MS/MS was carried out using a UPLC-MSD assembly consisting of an Accela AS injector, Accela UPLC pump, and a TSQ Vantage bench-top mass spectrometer (ThermoFisher Scientific, Waltham, MA) equipped with a heated electrospray probe (HESI). Each NAD⁺ metabolite (50 μM in 50% methanol) was infused at 10 μl/min by means of the Vantage syringe pump system into the MSD and subjected to collision induced dissociation in negative and positive ion mode to determine the most intense product ion (optimum sensitivity) and its associated collision voltage. These transitions (SRM) were subsequently set in the method MSD parameters. Hence, the MSD was operated in mixed polarity multiple reaction monitoring mode (MRM). All transitions and collision energies are shown in Table 1. Spectra were accumulated during 0.250 s per transition. MSD capillary voltage, capillary temperature and collision gas pressure (argon) were set to 4000 V, 300 °C and 1.0 Torr respectively. Sheath and auxiliary gas valves (nitrogen) were set at 20 and 10 arbitrary units.

Bustamante S., Jayasena T., Richani D., Gilchrist R.B., Wu L.E., Sinclair D.A., Sachdev P.S, & Braidy N. (2017). Quantifying the cellular NAD+ metabolome using a tandem liquid chromatography mass spectrometry approach. Metabolomics : Official journal of the Metabolomic Society, 14(1), 15.

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