Log In Sign up
The largest database of trusted experimental protocols

Prl tk renilla luciferase plasmid dna

Manufactured by Promega
Visit Supplier

The PRL-TK Renilla luciferase plasmid DNA is a laboratory tool that contains the Renilla luciferase gene. Renilla luciferase is a bioluminescent protein that can be used as a reporter to measure gene expression or other cellular events.

Automatically generated - may contain errors

Lab products found in correlation

Dual glo luciferase assay system, by Promega (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Close spelling variants of this product

PRL-TK Renilla luciferase PRL-TK Renilla Renilla Luciferase plasmid DNA PRL-TK plasmid Renilla-luciferase (pRL) Renilla-TK plasmid PRL-TK DNA Renilla luciferase plasmid Renilla Luciferase DNA PRL-TK

3 protocols using prl tk renilla luciferase plasmid dna

1

Dual-Luciferase Reporter Assay for Gastric Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell-based luciferase reporter assay, gastric cancer NCI-N87 and MKN-45 cells were plated in 6-well plates at ~50% confluency. The cells were co-transfected with 100 ng/well TOPflash or FOPflash plasmid DNA and 2 ng/well pRL-TK Renilla luciferase plasmid DNA (Promega, Madison, WI) in addition to empty vectors, shRNA for FAM83H, hSCRIB CRISPR/Cas9 KO plasmid, overexpression vector for FAM83H, or overexpression vector for SCRIB by using Lipofectamine® 2000 (Thermo Fisher Scientific, Waltham, MA). Twelve hours after transfection, cells were lysed with 1x Passive Lysis buffer using Dual-Glo Assay (Promega, Madison, WI). The firefly luciferase signals were monitored from 10 μl of each protein lysate by using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI), and normalized to the signals obtained from positive controls of co-transfected Renilla luciferase expression. The assay was performed in quadruplicate and repeated three times.
Hussein U.K., Ha S.H., Ahmed A.G., Kim K.M., Park S.H., Kim C.Y., Kwon K.S., Zhang Z., Lee S.A., Park H.S., Park B.H., Lee H., Chung M.J., Moon W.S., Kang M.J, & Jang K.Y. (2020). FAM83H and SCRIB stabilize β-catenin and stimulate progression of gastric carcinoma. Aging (Albany NY), 12(12), 11812-11834.
+ Open protocol
+ Expand
2

Luciferase Assay for PAX5 Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hek293T cells expressing pMC3-PAX5.HygroWT, pMC3-PAX5.HygroEmpty or pMC3-PAX5.Hygromutant were transfected with 2 µg luc-CD19 construct (kindly provided by M. Busslinger) (23 (link)) and 100 ng pRL-TK Renilla luciferase plasmid DNA (Promega) using FuGene 6 (Roche Diagnostics). 48 h after transfection, cells were lysed and Firefly and Renilla luciferase activity measurement was performed using the Dual-Glo Luciferase Assay System (Promega), according to manufacturer’s protocol. All transfections were carried out in triplicate in at least 3 independent experiments. Firefly luciferase activity was normalized according to the corresponding Renilla luciferase activity.
Martín-Lorenzo A., Auer F., Chan L.N., García-Ramírez I., González-Herrero I., Rodríguez-Hernández G., Bartenhagen C., Dugas M., Gombert M., Ginzel S., Blanco O., Orfao A., Alonso-López D., De Las Rivas J., García-Cenador M.B., García-Criado F.J., Müschen M., Sánchez-García I., Borkhardt A., Vicente-Dueñas C, & Hauer J. (2018). Loss of Pax5 exploits Sca1-BCR-ABLp190 susceptibility to confer the metabolic shift essential for pB-ALL. Cancer research, 78(10), 2669-2679.
+ Open protocol
+ Expand
3

Quantifying ERG Transcription Factor Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Twenty four hours after plating 20,000 293T cells per well in a 96-well plate, cells were transfected with equimolar amounts of ERG wild type (MIG-ERG isotype 1), mutant (MIG-ERG-e6alt) or MIG empty vector along with 250 ng of pGL3-gpIX luciferase reporter plasmid and 50 ng of pRL-TK Renilla luciferase plasmid DNA (Promega) using Fugene 6 Transfection Reagent (Roche Diagnostics, Alameda, CA). Competition assays were performed by transfecting increasing amounts of mutant ERG plasmid together with a fixed amount of wild type ERG plasmid or vice versa complemented with empty vector. Forty-eight hours post-transfection, cell lysis and measurement of firefly and Renilla luciferase activity was performed using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions. Transfections were performed in triplicate in at least two independent experiments. The firefly luciferase - Renilla luciferase ratio was reported as mean ± s.e.m.
Zhang J., McCastlain K., Yoshihara H., Xu B., Chang Y., Churchman M.L., Wu G., Li Y., Wei L., Iacobucci I., Liu Y., Qu C., Wen J., Edmonson M., Payne-Turner D., Kaufmann K.B., Takayanagi S.I., Wienholds E., Waanders E., Ntziachristos P., Bakogianni S., Wang J., Aifantis I., Roberts K.G., Ma J., Song G., Easton J., Mulder H.L., Chen X., Newman S., Ma X., Rusch M., Gupta P., Boggs K., Vadodaria B., Dalton J., Liu Y., Valentine M.L., Ding L., Lu C., Fulton R.S., Fulton L., Tabib Y., Ochoa K., Devidas M., Pei D., Cheng C., Yang J., Evans W.E., Relling M.V., Pui C.H., Jeha S., Harvey R.C., Chen I.M., Willman C.L., Marcucci G., Bloomfield C.D., Kohlschmidt J., Mrózek K., Paietta E., Tallman M.S., Stock W., Foster M.C., Racevskis J., Rowe J.M., Luger S., Kornblau S.M., Shurtleff S.A., Raimondi S.C., Mardis E.R., Wilson R.K., Dick J.E., Hunger S.P., Loh M.L., Downing J.R, & Mullighan C.G. (2016). DEREGULATION OF DUX4 AND ERG IN ACUTE LYMPHOBLASTIC LEUKEMIA. Nature genetics, 48(12), 1481-1489.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!