Sc 517464

Manufactured by Santa Cruz Biotechnology

Sc-517464 is a piece of laboratory equipment manufactured by Santa Cruz Biotechnology. It is designed for use in scientific research applications. The core function of this product is to provide a specialized tool for researchers to utilize in their experiments. Further details about the specific capabilities or intended use of Sc-517464 are not available.

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Lab products found in correlation

Ab134953, by Abcam (1 mentions) Ab30620, by Abcam (1 mentions) Ab16667, by Abcam (1 mentions) Human egf, by Merck Group (1 mentions) Pd153035, by Selleck Chemicals (1 mentions) Tucatinib, by MedChemExpress (1 mentions) Zm 0071, by ZSGB-BIO (1 mentions) Actinomycin d, by Avantor (1 mentions) Cycloheximide (chx), by Beyotime (1 mentions) Sp600125, by Merck Group (1 mentions) Mg132, by MedChemExpress (1 mentions) Gefitinib, by MedChemExpress (1 mentions) Anti phlpp2, by Abcam (1 mentions) Anti phospho akt s473, by Cell Signaling Technology (1 mentions) Anti mtor, by Santa Cruz Biotechnology (1 mentions) Anti pi3k p85α, by Santa Cruz Biotechnology (1 mentions) Anti phospho pi3k, by Cell Signaling Technology (1 mentions) Anti myc tag, by Cell Signaling Technology (1 mentions) Anti phospho bad, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions)

2 protocols using sc 517464

Investigating Signaling Pathways in Cell Lines

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Gefitinib, tucatinib and MG132 were obtained from MedChemExpress (HY-50895, HY-16069 and HY-13259). Actinomycin D was obtained from Amresco (J608). SP600125 and human EGF were purchased from Sigma-Aldrich (S5567 and E9644). PD153035 was purchased from Selleck (S6546). Cycloheximide was obtained from Beyotime (S1560).
The antibodies against human c-Jun, PARP-1, phosphorylated ERK1/2 (Thr202/Tyr204), phosphorylated mTOR (Ser2448), TSC1, JNK and phosphorylated JNK were from Cell Signaling Technology (9165, 9532, 4370, 5536, 6935, 9252 and 4668). The anti-CNOT3 antibody was purchased from Proteintech (11135-1-AP). The antibodies against phosphorylated c-Jun (S73), ki67 and ubiquitin were from Abcam (ab30620, Ab16667 and ab134953). The antibodies against ERK1/2 were purchased from Abcam (ab54230) and Cell Signaling Technology (4695). The antibody against mTOR was purchased from Santa Cruz Biotechnology (sc-517464). The antibody against Keratin 7 was obtained from ZSGB-BIO (ZM-0071).

Jing L., Zhai M.E., Qian M.R., Li Y.M., Han M.W., Wang K., Huang W., Nan G, & Jiang J.L. (2023). Targeting the up-regulated CNOT3 reverses therapeutic resistance and metastatic progression of EGFR-mutant non-small cell lung cancer. Cell Death Discovery, 9, 406.

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Comprehensive Western Blot Analysis

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Western blot was performed as previously described [27] with the following primary antibodies: anti-mTOR (1:100, sc-517464, Santa Cruz), anti-mTOR (1:1,000, #2972, Cell Signaling Technology), antiphospho-IGF-1 Receptor (1:1000, #3021, Cell Signaling Technology), anti-phospho-HER-2(1:100, sc-81507, Santa Cruz), anti-Protor1 (1:100, sc-390496, Santa Cruz), anti-DEPTOR (1:100, sc-398169, Santa Cruz), anti-PDK1 (1:200, sc-293160, Santa Cruz), anti-PI3K p85α (1:100, sc-1673, Santa Cruz), antiphospho-PI3K (1:100, sc-12929, Santa Cruz), anti-phospho-PI3K (1:1000, #4228, Cell Signaling Technology), anti-AKT (1:1,000, #4691T, Cell Signaling Technology), anti-phospho-Akt(S473) (1:1,000, #4060T, Cell Signaling Technology), anti-PHLPP2 (1:1,000, ab71973, Abcam), anti-phospho-Akt (T308) (1:100, sc-271966, Santa Cruz), anti-phospho-BAD (1:1000, #5284, Cell Signaling Technology), antiphospho-GSK3β(Ser9) (1:1,000, #9323, Cell Signaling Technology), anti-phospho-FoxO1(Ser256) (1:1000, #9461T, Cell Signaling Technology), anti-phospho-4EBP1 (1:200, sc-293124, Santa Cruz), antiphospho-p70S6K (1:200, sc-8416, Santa Cruz), anti-GAPDH (1:1,000, sc-47724, Santa Cruz), and antimyc-Tag (1:1,000, #2276s, Cell Signaling Technology).

Huang J., Li J., Tang J., Wu Y., Yi Z., Wang Y., Li Y., Tan Y., Ye L., Ren G., & Xiang T. (2021). ZDHHC22-Mediated mTOR Palmitoylation Determines Breast Tumor Growth and Endocrine Therapy Sensitivity.

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