Isotype control antibodies clone 2a3

Animal experiments were conducted in accordance with national and international policies, and our protocol was approved by the Regional Ethics Committee of Midi-Pyrénées. Untransfected or transfected Yumm cells (3.10⁵) were intradermally injected into the flank of 7-week-old C57BL/6 mice (Charles River, L’Arbresle, France) or NSG mice (a kind gift from Dr. JE. Sarry, INSERM U1037, Toulouse, France). Tumor volumes were measured every 2-3 days using a caliper^{8 (link)}. For ICI combination experiments, mice were injected i.p. three times with anti-CTLA-4 (200 µg per mouse on day 7 and 100 µg per mouse on day 10 and 13), or with anti-PD-1 or isotype control antibody (200 µg per mouse on day 5, 7 and 10). Anti-CTLA-4 (clone 9H10), anti-PD-1 (clone RMP1-14) and isotype control antibodies (clone 2A3) were purchased from BioXcell. For SKI-I treatment, mice were treated or not with 50 mg/kg SKI-I (N’-[(2-hydroxynaphthalen-1-yl) methylidene]-3-(naphthalen-2-yl)-1H-pyrazole-5-carbohydrazide, Enamine) by i.p. injection on days 5, 7, 10, 13, and 15.

A neutralizing anti-mouse PD-1 antibody was administered i.p. at 0.2 mg on days 4, 6, 9 and 11 after tumor graft. Anti-PD-1 antibody (clone RMP1-14) and isotype control antibodies (clone 2A3) were purchased from BioXcell (Supplied by Euromedex, Souffelweyersheim, France).