Amazon speed etd instrument

MS/MS experiments were carried out using a dual ion funnel AmaZon speed ETD instrument (Bruker Daltonics) operating in positive mode. HRGP330 was infused at a rate of 6 μL/min and acquisitions were performed using a scan speed of 8100 m/z per second. The following parameters were used: trap drive = 71.8; capillary exit = 140 V; source temperature = 453 K. An isolation width of 4 m/z was used to select the ion of interest and CID was performed by applying an amplitude of 1.0 V. Fluoranthene was used as the electron transfer dissociation reagent with an ETD reaction time of 100–120 ms, followed by a proton transfer reaction (PTR) time of 75 ms. Monoisotopic peaks were assigned using the SNAP™ peak detection algorithm. Spectra were analysed using BioTools v3.2 and Sequence Editor (Bruker Daltonics).

Electrospray Ionization Mass Spectrometry (ESI MS) of APE_B/D#3 was performed in negative ion mode using an amaZon speed ETD—Instrument (Bruker Daltonics) equipped with ESI ion source. Sample was dissolved at a concentration of ~10 ng μL−1 in 10 mM ammonium acetate (50/50, v/v) mixture of chloroform and methanol and sprayed at a flow rate of 3 μL min−1. Capillary entrance voltage was set to 4.5 kV, and dry gas temperature to 180°C.