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Lipopolysaccharide lps from escherichia coli serotype 026 b6

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Lipopolysaccharide (LPS) from Escherichia coli (serotype 026:B6) is a bacterial endotoxin, which is a component of the outer membrane of Gram-negative bacteria. It is a complex molecule consisting of a lipid and a polysaccharide.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Iscript kit, by Bio-Rad (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Sybr green, by Bio-Rad (1 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions) Alkaline phosphatase linked secondary antibodies, by GE Healthcare (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Trizma base, by Merck Group (1 mentions) Bovine thrombin, by Merck Group (1 mentions) Murine tf standard, by R&D Systems (1 mentions) Vwf antibody, by Agilent Technologies (1 mentions) Anti e selectin, by Santa Cruz Biotechnology (1 mentions) Potassium nitrate, by Merck Group (1 mentions) Streptavidin peroxidase, by Santa Cruz Biotechnology (1 mentions) Anti vcam 1, by Santa Cruz Biotechnology (1 mentions) Anti icam 1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

LPS (7203 citations) Lipopolysaccharide (LPS) (1055 citations) Lipopolysaccharide (576 citations) LPS from Escherichia coli (178 citations) Escherichia coli LPS (168 citations) Escherichia coli (94 citations) LPS from Escherichia coli 026:B6 (32 citations) LPS (Escherichia coli 026:B6) (18 citations) LPS (Escherichia coli serotype 026:B6; (17 citations) LPS from Escherichia coli serotype 026:B6 (13 citations)

6 protocols using lipopolysaccharide lps from escherichia coli serotype 026 b6

1

Anti-inflammatory Signaling Pathway Assay

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Lipopolysaccharide (LPS) from Escherichia coli (serotype 026:B6) was purchased at Sigma Chemical Co. (St. Louis, MO, United States). Fetal bovine serum (FBS) and trypsin were obtained from Invitrogen (Paisley, OR, United Kingdom). The protease and phosphatase inhibitor cocktails were obtained from Roche (Mannheim, Germany). Antibodies against phospho-IκB-α and IκB-α, were purchased from Cell Signaling Technologies (Danvers, MA, United States); against iNOS were from R&D Systems (Minneapolis, MN, United States) and against COX-1 and COX-2 from Abcam (Cambridge, United Kingdom). The anti-actin antibody was purchased from Millipore (Bedford, MA). The alkaline phosphatase-linked secondary antibodies and the enhanced chemifluorescence (ECF) reagent were obtained from GE Healthcare (Chalfont St. Giles, United Kingdom). The polyvinylidene difluoride (PVDF) membranes were from Millipore Corporation (Bedford, MA). Trizol® reagent was purchased from Invitrogen (Barcelona, Spain). iScript kit and SYBR green were obtained from BioRad (Hercules, CA, United States). Primers were from MWG Biotech (Ebersberg, Germany). The remaining reagents were from Sigma Chemical Co. (St. Louis, Mo, United States) or from Merck (Darmstadt, Germany).
Zuzarte M., Francisco V., Neves B., Liberal J., Cavaleiro C., Canhoto J., Salgueiro L, & Cruz M.T. (2022). Lavandula viridis L´Hér. Essential Oil Inhibits the Inflammatory Response in Macrophages Through Blockade of NF-KB Signaling Cascade. Frontiers in Pharmacology, 12, 695911.
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2

Vascular Inflammation Assay Protocol

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Cadmium granules, potassium nitrate, bovine thrombin, sodium chloride, calcium chloride, lipopolysaccharide (LPS, from Escherichia coli serotype 026: B6), trichloroacetic acid, trizma base, sulphorhodamine B, 3′diaminobenzidine, HEPES, and other reagents were purchased from Sigma Aldrich, USA. Avidin peroxidase and streptAvidin peroxidase, anti-VCAM-1, anti-ICAM-1, anti-E-Selectin antibodies were obtained from Santa Cruz Biotechnology, USA. 3,3′,5,5′-tetramethylbenzidine (TMB) was purchased from Scyteck, USA. Chromogenic substrates from Chromogenix AB, Italy. Plasmin, uPA and double chain tPA standards (tcu-PA) were obtained from Sekisui, USA. Mouse IL-6 minikit and mouse TNF-α minikit, recombinant mouse TNF-α were purchased from Thermo Scientific, USA. Fetal bovine serum from Gibco, BRL, USA. vWF antibody from DAKO, USA. Murine TF standard were obtained from R&D Systems, USA.
Salazar E., Salazar A.M., Taylor P., Urdanibia I., Pérez K., Rodríguez-Acosta A., Sánchez E.E, & Guerrero B. (2019). Contribution of endothelial cell and macrophage activation in the alterations induced by the venom of Micrurus tener tener in C57BL/6 mice. Molecular immunology, 116, 45-55.
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3

Cytokine Expression in PBMC Cultures

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PBMC (1 x 106 cells/ml) cultures were carried out in RPMI-1640 (Sigma-Aldrich-Merck, Darmstadt, Germany) supplemented with: 10% FBS, 100 U/ml penicillin, 100 µg/ml gentamycin and 0.3 mg/ml L-glutamine. The cells were stimulated with: 30 µg /ml C3 binding glycoprotein (C3bgp) isolated as described previously [14 (link)] or 1 µg/ml Lipopolysaccharide (LPS) from Escherichia coli serotype 026: B6 (Sigma-Aldrich-Merck, Darmstadt, Germany). The concentrations of the stimuli used were determined, according to Stanilova et al., [15 ] and Takahashi et al., [16 (link)]. As shown by our previous study of inducible cytokine gene expression transcripts, the maximum mRNA quantities were reached after 6 h of stimulation and were independent of stimuli used [5 (link)]. Therefore, PBMC cultures were incubated at 37°C for 6 h. After incubation, the cultures were centrifuged at 1800 rpm for 10 min. The cell pellet was separated, and the RNA was isolated.
Stanilova S.A., Grigorov B.G., Platikanova M.S, & Dobreva Z.G. (2019). Effect of a Small Selective Inhibitor of C-Jun N-Terminal Kinase on the Inducible mRNA Expression of Interleukin-6 and Interleukin-18. Open Access Macedonian Journal of Medical Sciences, 7(13), 2062-2067.
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4

Analgesic and Anti-inflammatory Evaluation

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Experiments were performed on male Wistar rats housed in controlled conditions. The extracts were administered intragastrically (p.o.) at a dose of 200 mg/kg b.w. (groups FEW, PEF, NPF); morphine (5 mg/kg b.w., s.c.) and diclofenac (50 mg/kg b.w., i.p.) were used as standard analgesic drugs, control animals were treated with vehiculum (water) (p.o.). In order to measure locomotor activity (n = 53) and to perform the hot plate test (n = 40) 6 groups of rats were used (FEW, PEF, NPF, morphine, diclofenac 50, vehiculum (water) (p.o.). Anti-inflammatory activity was measured in a model of carrageenan-induced raw paw edema using 5 groups of rats (FWE, PEF, NPF, diclofenac, vehiculum (water)). Lipopolysaccharide (LPS) from Escherichia coli serotype 026:B6, (Sigma Chemicals Co.) was dissolved in an aqua pro injectione and administered in a single dose of 100 µg/kg intraperitoneally (i.p.) according to Manikowska et al. [19 (link)].
Mikołajczak P.Ł., Kędzia B., Ożarowski M., Kujawski R., Bogacz A., Bartkowiak-Wieczorek J., Białas W., Gryszczyńska A., Buchwald W., Szulc M., Wasiak N., Górska-Paukszta M., Baraniak J., Czerny B, & Seremak-Mrozikiewicz A. (2016). Evaluation of anti-inflammatory and analgesic activities of extracts from herb of Chelidonium majus L.. Central-European Journal of Immunology, 40(4), 400-410.
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5

Neurochemical Signaling Pathway Analysis

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Methanol, hexane, chloroform, ethyl acetate, butanol, lipopolysaccharide (LPS) from Escherichia coli serotype 026:B6 (Lot# 021M4072V), (-)-nicotine, methyllycaconitine citrate salt hydrate (MLA) from Delphinium brownii seeds, mecamylamine hydrochloride and 7-hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt (resazurin) were purchased from Sigma-Aldrich (St Louis, MO, USA). Astragalin, baicalein, catechin, daidzin, daidzein, delphinidin, genistein, isoquercitrin, isorhamnetin, malvidin, petunidin-3-glycoside, quercetagetin, quercetin, rhamnetin, sakuranetin, spiraeoside and tamarixetin (Fig. 1) were purchased from Chromadex (Irvine, CA, USA). [3H]-MLA (∼60Ci/mmol), [3H]-cytisine (∼16Ci/mmol) and [3H]-epibatidine (∼30Ci/mmol) were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Antibiotics (10000U/mL penicillin and 10000ug/mL streptomycin), 2.5% trypsin (10×), fetal bovine serum (FBS), Dulbeccos' Modified Eagle Medium (DMEM), DMEM:Nutrient mixture F-12 (DMEM/F-12) and Hanks Balanced Salt Solution (HBSS) were purchased from Life Technologies Corporation (Grand Island, NY, USA). Griess reagent system was purchased from Promega Corporation (Madison, WI, USA). TNF-alpha ELISA Ready-SET-Go!® was purchased from eBioscience Inc. (San Diego, CA, USA).
Lutz J.A., Kulshrestha M., Rogers D.T, & Littleton J.M. (2014). A nicotinic receptor-mediated anti-inflammatory effect of the flavonoid rhamnetin in BV2 microglia. Fitoterapia, 98, 11-21.
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6

Calendula Extract Modulates Inflammatory Response

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The calendula flower extract was purchased from Acofarma (Madrid, Spain). It consists of an extract from marigold flowers in refined soy oil, stabilized with tocopherol. The INCI designation of this cosmetic ingredient is "Calendula officinalis L. flower extract". Lipopolysaccharide (LPS) from Escherichia coli (serotype 026:B6) was obtained from Sigma Chemical Co. (St. Louis, MO, USA) and trypsin and fetal calf serum were purchased from Invitrogen (Paisley, UK). All of other reagents were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
Silva D., Ferreira M., Lobo J.M.S., Cruz M.T., & Almeida I.F. (2021). Anti-Inflammatory Activity of Calendula officinalis L. Flower Extract. Cosmetics, 8(2), 31-31.
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