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Diaminobenzidine peroxidase horse radish peroxidase substrate

Manufactured by Vector Laboratories

Diaminobenzidine (DAB) is a peroxidase substrate used as a chromogenic detection system. It is commonly used in immunohistochemistry, in situ hybridization, and other applications that involve the detection of target analytes using a peroxidase-conjugated detection system.

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2 protocols using diaminobenzidine peroxidase horse radish peroxidase substrate

1

Xenograft Tissue Staining Protocol

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Paraffin sections of the xenografts were stained with UEA I (Ulex europaeus Agglutinin I;1:100, Vector Laboratories). This was followed by peroxidase-conjugated secondary antibody and staining with diaminobenzidine peroxidase (horse-radish peroxidase) substrate (Vector Laboratories). Slides were counterstained using hematoxylin (Vector Laboratories). Images were captured using a Nikon microscope.
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2

Immunohistochemical Analysis of VM Lesions

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Paraffin sections of murine VM lesions were stained with UEA I (Ulex europaeus Agglutinin I; Vector Laboratories), cleaved caspase-3 (Cell Signaling), and anti-human Ki-67 (Abcam). This was followed by peroxidase-conjugated secondary antibody and staining with diaminobenzidine peroxidase (horseradish peroxidase) substrate (Vector Laboratories). Slides were counterstained using hematoxylin (Vector Laboratories).
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