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Isocage biocontainment isolator cages

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The ISOcage biocontainment isolator cages are specialized lab equipment designed to provide a controlled and isolated environment for various research and experimentation purposes. The cages offer a physical barrier to contain and isolate specimens, materials, or procedures within a secure and sterile workspace.

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Isocages (17 citations)

2 protocols using isocage biocontainment isolator cages

1

Gnotobiotic Colonization of Mice with Altered Schaedler Flora

Cited 1 time
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C57BL/6 mice were maintained in germ-free conditions in ISOcage biocontainment isolator cages (Tecniplast, PA, USA) in the gnotobiotic facility at Rockefeller University. ASF colonization was achieved by inoculating germ-free mice with cecal contents of ASF donor mice stably colonized by vertical transmission (kindly provided by Amanda Ramer-Tait, University of Nebraska-Lincoln). Ceca were prepared by homogenization through a 100 μm filter in sterile phosphate-buffered saline (PBS) at a ratio of one cecum per 1 ml of PBS. Mice received 200 μl of ASF inoculum via oral-gavage twice, one week apart. The presence of members of the ASF microbial community was confirmed by a real-time PCR-based assay previously described34 (link). All mice were analysed at least four weeks post colonization, with colonization further confirmed by 16s RNA sequencing of both faeces and ceca of analysed mice. We could detect RNA of at least 6 species by qPCR and 16s RNA sequencing after colonization.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
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2

Gnotobiotic Colonization of Mice with Altered Schaedler Flora

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 mice were maintained in germ-free conditions in ISOcage biocontainment isolator cages (Tecniplast, PA, USA) in the gnotobiotic facility at Rockefeller University. ASF colonization was achieved by inoculating germ-free mice with cecal contents of ASF donor mice stably colonized by vertical transmission (kindly provided by Amanda Ramer-Tait, University of Nebraska-Lincoln). Ceca were prepared by homogenization through a 100 μm filter in sterile phosphate-buffered saline (PBS) at a ratio of one cecum per 1 ml of PBS. Mice received 200 μl of ASF inoculum via oral-gavage twice, one week apart. The presence of members of the ASF microbial community was confirmed by a real-time PCR-based assay previously described34 (link). All mice were analysed at least four weeks post colonization, with colonization further confirmed by 16s RNA sequencing of both faeces and ceca of analysed mice. We could detect RNA of at least 6 species by qPCR and 16s RNA sequencing after colonization.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
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