Rabbit anti pi3k

Manufactured by Proteintech

Sourced in China

Rabbit-anti-PI3K is a primary antibody that specifically recognizes the phosphoinositide 3-kinase (PI3K) protein in rabbit samples. This antibody can be used in various immunological techniques to detect and study the expression of PI3K.

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Lab products found in correlation

Rabbit anti p akt, by Affinity Biosciences (2 mentions) Rabbit anti egfr, by Huabio (2 mentions) Rabbit anti akt, by Proteintech (2 mentions) Rabbit anti cav 1, by Proteintech (2 mentions) Rabbit anti gapdh, by Proteintech (2 mentions) Rabbit anti vegfr2, by Affinity Biosciences (1 mentions) Af4426, by Affinity Biosciences (1 mentions) Supersignal west dura chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Rabbit anti rbsn, by Merck Group (1 mentions) Goat anti eea1, by Santa Cruz Biotechnology (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

Spelling variants of this product

Anti-PI3K (12 citations) Anti-TM (2 citations)

3 protocols using rabbit anti pi3k

Western Blot Analysis of Signaling Proteins

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Protein samples were subjected to western blot according to our previously described methods (Yao et al., 2019 (link)). The following antibodies were used: rabbit-anti-p-Cav-1 (AF3386; Affinity), rabbit-anti-Cav-1 (cat#16447-1-AP; Proteintech), rabbit-anti-p-PI3K (AF3241; Affinity), rabbit-anti-PI3K (cat#20584-1-AP; Proteintech), rabbit-anti-p-AKT (AF4418; Affinity), rabbit-anti-AKT (cat#10176-2-AP; Proteintech), rabbit-anti-p-EGFR (cat#ET1606-44; HuaBio), rabbit-anti-EGFR (cat#ET1603-37; HuaBio), rabbit-anti- Angiopoietin1 (AF5184; Affinity) , rabbit-anti- p-VEGFR2 (AF4426; Affinity), rabbit-anti- VEGFR2 (AF4726; Affinity), rabbit-anti-GAPDH (cat#10494-1-AP; Proteintech). Then, the blots were visualised by exposure to X-ray film.

Jia J., He R., Yao Z., Su J., Deng S., Chen K, & Yu B. (2023). Daidzein alleviates osteoporosis by promoting osteogenesis and angiogenesis coupling. PeerJ, 11, e16121.

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Protein Extraction and Western Blot Analysis

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Cells were lysed in RIPA extraction buffer supplemented with 1 mM dithiothreitol (DTT) and 1X Protease Inhibitor Cocktail (Roche, Basel, Switzerland). Protein concentrations of cleared lysates were measured using the BCA assay before an equal amount of protein was loaded on precast 4-20% Tris/Glycine/SDS polyacrylamide gradient gels (BioRad). Transferred PVDF membranes were blotted using antibodies against: 1:100 rabbit anti-HACL1
(GeneTex, Irvine, CA; GTX106858), 1:4000 mouse anti-GAPDH (Santa-Cruz Biotechnology, Dallas, TX; sc-47724), 1:250 polyclonal rabbit anti-RBSN (Sigma-Aldrich, St. Louis, MO; HPA044878), 1:100 rabbit anti-PI3K (Proteintech, Rosemont, IL; 20584-1-AP), 1:100 goat anti-EEA1 (Santa-Cruz; sc-6415), 1:55 rabbit anti-CLTA (Sigma-Aldrich; HPA050918), 1:200 anti-CTSD (GeneTtex; GTX62063, Abcam; ab6313, Santa-Cruz; sc-6486), 1 µg/ml mouse anti-TFRC (Life Technologies / Thermofisher Scientific; 13-6800). Secondary anti-mouse-HRP, anti-goat-HRP and anti-rabbit-HRP were used at 1:4000 before visualization on x-ray film with SuperSignal West Dura Chemiluminescent Substrate (ThermoFisher Scientific).

Paul F., Ng C., Mohamad Sahari U.B., Nafissi S., Nilipoor Y., Tavasoli A.R., Bonnard C., Wong P.M., Nabavizadeh N., Altunoğlu U., Estiar M.A., Majoie C.B., Lee H., Nelson S.F., Gan-Or Z., Rouleau G.A., Van Veldhoven P.P., Massie R., Hennekam R.C., Kariminejad A, & Reversade B. (2022). RABENOSYN separation-of-function mutations uncouple endosomal recycling from lysosomal degradation, causing a distinct Mendelian disorder. Human molecular genetics, 31(21).

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Investigating Bone and Endothelial Markers in Erlotinib and Daidzein Treatment

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rat-anti-endomucin (EMCN) (sc-65495; Santa Cruz Biotechnology, Dallax, TX, USA), tartrate resistant acid phosphatase (TRAP) (No:294-67001; Wako, Richmond, VA, USA), rabbit-anti-osteocalcin (OCN) (DF12303; Affinity, Jiangsu, China), erlotinib (HY-50896; MechemExpress, Monmouth Junction, NJ, USA), daidzein (cas#486-66-8; MechemExpress, Monmouth Junction, NJ, USA); rabbit-anti-p-Cav-1 (AF3386; Affinity, Jiangsu, China), rabbit-anti-Cav-1 (cat#16447-1-AP; Proteintech, Wuhan, China), rabbit-anti-p-PI3K (AF3241; Affinity, Jiangsu, China), rabbit-anti-PI3k (cat#20584-1-AP; Proteintech, Wuhan, China), rabbit-anti-p-AKT (AF4418; Affinity, Jiangsu, China), rabbit-anti-AKT (cat#10176-2-AP; Proteintech, Wuhan, China), rabbit-anti-p-epidermal growth factor receptor (EGFR) (cat#ET1606-44; HuaBio, Hangzhou, China), rabbit-anti-EGFR (cat#ET1603-37; HuaBio, Hangzhou, China), rabbit-anti-GAPDH (cat#10494-1-AP; Proteintech, Wuhan, China) antibodies.

Jia J., He R., Yao Z., Su J., Deng S., Chen K, & Yu B. (2023). Daidzein alleviates osteoporosis by promoting osteogenesis and angiogenesis coupling. PeerJ, 11, e16121.

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