Plasma adiponectin (Millipore, USA. Cat # RAB1136-1KT) and insulin (Millipore, USA. Cat # RAB0904-1KT) were assessed by sandwich ELISA following the manufacturer's protocol. Enzyme-based commercially available assay kits (Biosystems S.A., Spain) were used to measure total cholesterol (Cat # 11,505), triglycerides (Cat # 11,528) and HDL-cholesterol (Cat # 11,648) in circulation following the instructions provided by the manufacturer. Stress parameters such as TAC and catalase activity were determined in plasma using a spectrophotometric approach following the instructions given in the kits, which were purchased from BioAssay Systems, USA (Cat # DTAC-100 and # ECAT-100). Blood testosterone levels were analyzed by ELISA following the instructions provided with the kit (DRG, Cat # EIA-5179). Rat cytokine/chemokine profiles, including those of the rat adipocytokine leptin, interleukin-6 (IL-6), interleukin-1 beta (IL-1β), monocyte chemoattractant protein-1 (MCP-1, CCL2) and tumor necrosis factor-alpha (TNF-α), were analyzed in plasma samples with a Milliplex rat-cytokine immunoassay kit (Millipore, USA). Cat # RECYTMAG-65 K-08) according to the manufacturer's instructions.
Milliplex rat cytokine immunoassay kit
Manufactured by Merck Group
Sourced in United States
The Milliplex rat-cytokine immunoassay kit is a multiplex assay designed to measure the concentrations of multiple rat cytokines simultaneously in a single sample. The kit utilizes Luminex technology to quantify the levels of various cytokines, providing a comprehensive analysis of the cytokine profile in rat samples.
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Lab products found in correlation
2 protocols using milliplex rat cytokine immunoassay kit
1
Comprehensive Metabolic Biomarker Profiling
2
Cytokine Levels in Adipose Tissue
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The adipocytokines such as leptin, macrophage inflammatory protein-1 alpha (MIP-1α, CCL3), interleukin-4 (IL-4), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), macrophage chemo attractant protein-1 (MCP-1, CCL2), IFN-gamma-inducible protein 10 (IP-10, CXCL10), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-α) were estimated both in circulation as well as in visceral adipose tissue by Milliplex Rat cytokine immunoassay kit (Millipore, USA) according to manufacturer’s instructions.
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