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Omnis autostainer

Manufactured by StatLab
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The Omnis autostainer is a compact and automated instrument designed for immunohistochemistry (IHC) and in situ hybridization (ISH) applications. It performs slide processing and staining with precision and consistency.

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Lab products found in correlation

Clearify, by StatLab (4 mentions) Flex trs high, by Agilent Technologies (4 mentions) Rabbit linker, by Agilent Technologies (1 mentions) Caspase 3 antibody, by Cell Signaling Technology (1 mentions) Mouse linker, by Agilent Technologies (1 mentions)

4 protocols using omnis autostainer

1

Immunohistochemical Detection of VEGFR3

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Formalin-fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 15 minutes. Slides were incubated with VEGFR3 antibody (BD Pharmigen; catalog# 552857) for 30 minutes at 1/40 (3.13 IgG). Rabbit Anti-Rat (Agilent; E0468) which was applied for 30 minutes followed by Rabbit Envision (Agilent K4003) for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then put into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.
Vroom J.M., De Grauw K.J., Gerritsen H.C., Bradshaw D.J., Marsh P.D., Watson G.K., Birmingham J.J, & Allison C. (1999). Depth Penetration and Detection of pH Gradients in Biofilms by Two-Photon Excitation Microscopy. Applied and Environmental Microbiology, 65(8), 3502-3511.
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2

Caspase 3 Immunohistochemistry on FFPE Tissues

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Formalin-fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Slides were incubated with Caspase 3 antibody (Cell Signaling, 9661) for 30 minutes at 1/50 (1.26 IgG). Rabbit Linker (Dako, GV809) was applied for 10 mins followed by Rabbit Envision (Dako #K4003) for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then placed into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.
Vroom J.M., De Grauw K.J., Gerritsen H.C., Bradshaw D.J., Marsh P.D., Watson G.K., Birmingham J.J, & Allison C. (1999). Depth Penetration and Detection of pH Gradients in Biofilms by Two-Photon Excitation Microscopy. Applied and Environmental Microbiology, 65(8), 3502-3511.
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3

Immunohistochemical Staining of CD163

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Formalin and zinc fixed paraffin sections were cut at 4μm, placed on charged slides, and dried at 60°C for one hour. Slides were cooled to room temperature and added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL ) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Slides were incubated with CD163 antibody (Abcam, ab182422) for 30 minutes at 1/300 (2.24 IgG). Rabbit Envision (Dako #K4003) was applied for 30 minutes. DAB (Diaminobenzidine) (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with Hematoxylin for 8 minutes then placed into water. After removing slides from the Omnis they are dehydrated, cleared and coverslipped.
Vroom J.M., De Grauw K.J., Gerritsen H.C., Bradshaw D.J., Marsh P.D., Watson G.K., Birmingham J.J, & Allison C. (1999). Depth Penetration and Detection of pH Gradients in Biofilms by Two-Photon Excitation Microscopy. Applied and Environmental Microbiology, 65(8), 3502-3511.
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4

Immunohistochemical Staining of CD8+ T-Cells

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TMA sections were added to the Dako Omnis autostainer, where they were deparaffinized with Clearify (American Mastertech; catalog #CACLEGAL) and rinsed in water. Flex TRS High (Dako; catalog #GV804) was used for target retrieval for 30 minutes. Sections were incubated with CD8 (Dako #M7103) for 10 minutes at 1/50. Mouse Linker (Dako #GV821) was applied for 10 minutes followed by horseradish peroxidase for 15 mins (Dako GV823). Diaminobenzidine (Dako; catalog #K3468) was applied for 5 minutes for visualization. Slides were counterstained with hematoxylin for 8 minutes then placed into water. Sections were dehydrated, cleared and coverslipped.
Yang Y., Attwood K., Bshara W., Mohler J.L., Guru K., Xu B., Kalinski P, & Chatta G. (2020). High intratumoral CD8+ T-cell infiltration is associated with improved survival in prostate cancer patients undergoing radical prostatectomy. The Prostate, 81(1), 20-28.
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