Sealsafe plus gm500 cages

Subjects were 12 young adult (2–4 months old) CBA/CaJ mice (4 males, 7 females), used longitudinally as both control and treated animals. Initial testing did not include tone intensity functions or certain frequencies of the GIN paradigm; therefore some tests are reported with n = 8. Mice were obtained from the Jackson Laboratory (Bar Harbor, ME) at 6 weeks of age and housed 3–4 per cage with litter-mates in Sealsafe Plus GM500 cages (36 x 16 x 13 cm) connected to a Aero70 Techniplast Smart Flow system (West Chester, PA). The housing cages were maintained at a constant temperature and humidity, using a 12 hour light cycle with water and food pellets available ad libitum. All procedures were approved by the Institutional Animal Care and Use Committee at the University of South Florida (IACUC #M3847).

CBA/CaJ mice, aged 4–8 months, were bred in house and housed 3–4 mice per cage with litter-mates in Sealsafe Plus GM500 cages (36.9 × 15.6 × 13.2 cm) connected to an Aero70 Techniplast Smart Flow system (West Chester, PA, United States). Animal housing was maintained at 24 ± 1°C, with a relative humidity range of 65 ± 3%, and a 12-h light cycle (on at 0700 h). Tap water and mouse chow were available ad libitum. All procedures were performed between 0800 and 1,600 h. Study procedures were performed in accordance with the National Institutes of Health’s Guide for the Care and Use of Laboratory Animals, and were approved by the Institutional Animal Care and Use Committee (#IS00000245) at the University of South Florida.