For western blotting, the following antibodies were used: ALK (Purchased from Cell Signaling Technologies; catalogue no. 3633; dilution: 1:1,000), phospho-ALK (Tyr1278) (Cell Signaling Technologies; 6941; 1:250), phospho-ALK (Tyr1604) (Cell Signaling Technologies; 3341; 1:250), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technologies; 4370; 1:2,000), p44/42 MAPK (Erk1/2) (Cell Signaling Technologies; 9102; 1:1,000), β-actin (Sigma-Aldrich; A-5441; 1:2,000), GAPDH (GENETEX; GTX100118; 1:2000). For the cell growth assay, Crizotinib (Sigma-Aldrich; PZ0191-5MG), DMSO (Sigma-Aldrich; D8418-100ML), and IL-3 (Peprotech; AF-213-13) were used.
Phospho alk tyr1278
Manufactured by Cell Signaling Technology
Phospho-ALK (Tyr1278) is a lab equipment product that detects the phosphorylation of the Anaplastic Lymphoma Kinase (ALK) protein at the tyrosine 1278 residue. This product is used to assess the activation state of the ALK protein.
Automatically generated - may contain errors
Lab products found in correlation
Crizotinib, by Merck Group (1 mentions)
Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions)
P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
Phospho alk tyr1604, by Cell Signaling Technology (1 mentions)
Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
Gapdh, by GeneTex (1 mentions)
Alectinib, by Selleck Chemicals (1 mentions)
Lorlatinib, by Selleck Chemicals (1 mentions)
Brigatinib, by Selleck Chemicals (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Ceritinib, by Selleck Chemicals (1 mentions)
Phospho γh2ax, by Cell Signaling Technology (1 mentions)
Phospho chk1, by Cell Signaling Technology (1 mentions)
Phospho akt s473, by Cell Signaling Technology (1 mentions)
Goat anti mouse immunoglobulin g igg, by Thermo Fisher Scientific (1 mentions)
Erk1 2 antibody, by BD (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
2 protocols using phospho alk tyr1278
1
Western Blotting Antibodies and Cell Growth Assay Reagents
2
Immunoblotting of ALK and AKT Signaling
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Cells were lysed on ice using hypotonic lysis buffer and immunoblotted as previously described [18] . Primary antibodies: phospho-ALK Tyr1278 (1:1,000; #3710S), pan-ALK D5F3 (1:5,000; #3633S), phospho-AKT S473 (1:5,000; #4060L), AKT (1:1,000; #9272), phospho-ERK1/2 (1:2,000; #4377S), PARP (1:1,000; #9542), RRM2 (1:1,000; #65939), phospho-Chk1 (1:1,000; #2348), phospho-γH2A.X (1:5,000; #9718), Tubulin (1:20,000; #2148) and GAPDH (1:20,000; #5147) were obtained from Cell Signaling Technology; while ERK1/2 antibody (1:5,000; #610124) was purchased from BD Transduction Laboratories. Horseradish peroxidase (HRP)-conjugated, goat anti-mouse immunoglobulin G (IgG), and goat anti-rabbit IgG (1:5,000) secondary antibodies were obtained from ThermoFisher scientific. Alectinib, brigatinib, lorlatinib, crizotinib, and ceritinib were purchased from Selleck Chemicals LLC (Houston, TX, USA).
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