We prepared particular probes to hsa_circ_0074298 (Dig-5′-GCCTCAACCACGGAGTTCCTTTTGCTCGG-3′-Dig) by Geneseed Biotech (Guangzhou, China). We detected signals using uorescein isothiocyanate (FITC)-conjugated anti-biotin antibodies and Cy3-conjugated anti-digoxin antibody (Jackson ImmunoResearch, West Grove, PA, USA). We counterstained nuclei with 4,6-diamidino-2-phenylindole, and obtained images using a Zeiss LSM 700 confocal microscope (Carl Zeiss, Oberkochen, Germany).
Uorescein isothiocyanate fitc conjugated anti biotin antibodies
Manufactured by Jackson ImmunoResearch
Sourced in United States
Fluorescein isothiocyanate (FITC)-conjugated anti-biotin antibodies are laboratory reagents used for the detection and visualization of biotin-labeled molecules. These antibodies are conjugated with the fluorescent dye FITC, which allows for the specific labeling and identification of biotin-containing targets in various experimental procedures, such as immunohistochemistry, flow cytometry, and Western blotting.
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2 protocols using uorescein isothiocyanate fitc conjugated anti biotin antibodies
1
Detecting hsa_circ_0074298 in Cells
2
Detecting hsa_circ_0074298 in Cells
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We prepared particular probes to hsa_circ_0074298 (Dig-5′-GCCTCAACCACGGAGTTCCTTTTGCTCGG-3′-Dig) by Geneseed Biotech (Guangzhou, China). We detected signals using uorescein isothiocyanate (FITC)-conjugated anti-biotin antibodies and Cy3-conjugated anti-digoxin antibody (Jackson ImmunoResearch, West Grove, PA, USA). We counterstained nuclei with 4,6-diamidino-2-phenylindole, and obtained images using a Zeiss LSM 700 confocal microscope (Carl Zeiss, Oberkochen, Germany).
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