Sigmastat 3.5 advisory statistics for scientists

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Sourced in United Kingdom

SigmaStat® 3.5 is a statistical software package designed for scientists. It provides advanced statistical analysis tools to aid in data interpretation and decision-making.

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SigmaStat 5.0 SigmaStat 3.5 SigmaStat

2 protocols using sigmastat 3.5 advisory statistics for scientists

Statistical Analysis of Densitometric Data

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Statistical analysis of the data was performed using SigmaStat^® 3.5 Advisory Statistics for Scientists (Systat Software, Richmond, CA) and Analyse-it (Analyse-it Software Ltd., UK). Densitometric data were normalized to lamin B1 (loading control) and next analyzed by one-way ANOVA. When ANOVA’s upshots were significant (p < 0.05) we used post hoc Tukey’s test for all pairwise comparisons and multiple comparisons vs. untreated control values. Null hypotheses were rejected when p < 0.05.

Chiarini A., Armato U., Hu P, & Dal Prà I. (2020). CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Aβ-Exposed Human Cortical Astrocytes. Cells, 9(6), 1386.

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Immunoblotting Analysis Protocol

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The data were analyzed using Sigma Stat 3.5™ Advisory Statistics for Scientists (Systat Software). For immunoblotting, bands’ densitometric data were normalized to matching loading control (lamin B1) bands and next analyzed by one–wayANOVA. When the ANOVA’s upshot was significant (P < 0.05), Bonferroni t-test, was used for all pairwise comparisons and multiple comparisons versus 0-h (untreated) control values. Null hypotheses were rejected when P > 0.05.

Chiarini A., Armato U., Liu D, & Dal Prà I. (2017). Calcium-Sensing Receptor Antagonist NPS 2143 Restores Amyloid Precursor Protein Physiological Non-Amyloidogenic Processing in Aβ-Exposed Adult Human Astrocytes. Scientific Reports, 7, 1277.

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