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Rabbit anti tuj1 antibody

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Rabbit anti-Tuj1 antibody is a primary antibody that recognizes the Tuj1 (Tubulin beta-3 chain) protein, which is a marker for neuronal cells. This antibody is commonly used in research applications to identify and study neurons.

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Close spelling variants of this product

Anti-Tuj1 (23 citations) Rabbit anti-Tuj1 (22 citations) Anti-TUJ1 antibody (3 citations) Rabbit Tuj1 (2 citations)

2 protocols using rabbit anti tuj1 antibody

1

Differentiation of Neurons and Astrocytes

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To generate neurons, OKSM-iNSCs were grown in NSC medium following removal of the growth factors bFGF and EGF for 5–10 days; neuronal cells were identified by immunostaining with rabbit anti-Tuj1 antibody (Covance, IgG1, 1:200). Astrocytes were generated using NSC medium supplemented with 5% FBS for a duration of 10 days, after which they were stained with rabbit anti-GFAP antibody (Dako Z0334, IgG, 1:200). The secondary antibody used was donkey anti-rabbit IgG (A10040, Alexa Fluor 546) at a concentration of 1:250. See “Immunocytochemistry” section for a detailed protocol.
Bar-Nur O., Verheul C., Sommer A.G., Brumbaugh J., Schwarz B.A., Lipchina I., Huebner A.J., Mostoslavsky G, & Hochedlinger K. (2015). Lineage conversion induced by pluripotency factors involves transient passage through an iPS cell stage. Nature biotechnology, 33(7), 761-768.
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2

Differentiation of Neurons and Astrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate neurons, OKSM-iNSCs were grown in NSC medium following removal of the growth factors bFGF and EGF for 5–10 days; neuronal cells were identified by immunostaining with rabbit anti-Tuj1 antibody (Covance, IgG1, 1:200). Astrocytes were generated using NSC medium supplemented with 5% FBS for a duration of 10 days, after which they were stained with rabbit anti-GFAP antibody (Dako Z0334, IgG, 1:200). The secondary antibody used was donkey anti-rabbit IgG (A10040, Alexa Fluor 546) at a concentration of 1:250. See “Immunocytochemistry” section for a detailed protocol.
Bar-Nur O., Verheul C., Sommer A.G., Brumbaugh J., Schwarz B.A., Lipchina I., Huebner A.J., Mostoslavsky G, & Hochedlinger K. (2015). Lineage conversion induced by pluripotency factors involves transient passage through an iPS cell stage. Nature biotechnology, 33(7), 761-768.
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