Agilent sureselect human all exome v6

Genomic DNA of COVID-19 patients was extracted from peripheral blood using a Maxwell^® RSC Blood DNA Kit (Promega, Madison, WI, USA), and DNA concentration and purity were evaluated using a NanoDrop™ 8000 spectrophotometer. For library preparation, we measured the DNA concentration with a Qubit^® DNA Assay Kit with a Qubit^® 2.0 fluorometer (Life Technologies by Thermo Fisher Scientific, Waltham, MA, USA), and 1.0 μg of DNA was used for library preparation. Genomic DNA was sonicated to generate 180–250-bp fragments and then end-polished, A-tailed, and ligated with the full-length adapter with further PCR amplification. PCR products were purified using the AMPure XP system (Beckman Coulter, Brea, CA, USA) and quantified using the Agilent high-sensitivity DNA assay from the Agilent TapeStation 4200 system (Agilent Technologies, Santa Clara, CA, USA). The whole exome was captured with Agilent SureSelect Human All Exome V6 (Agilent Technologies, Santa Clara, CA, USA), and the sequencing was performed on an Illumina NovaSeq 6000 System (Illumina, San Diego, CA, USA).

We collected COVID-19 samples from several hospitals in Southern Italy: Azienda ospedaliera specialistica dei colli, Monaldi-Cotugno; Azienda Ospedaliera di Rilievo Nazionale Antonio Cardarelli, P.O. Ospedale Boscotrecase, Villa dei Fiori S.r.l.- Acerra. The genomic DNA of COVID-19 patients was extracted from peripheral blood using a Maxwell^® RSC Blood DNA Kit (Promega, Madison, WI, USA), and DNA concentration and purity were evaluated using a NanoDrop™ 8000 Spectrophotometer. For library preparation, we measured the DNA concentration of 147 COVID-19 patients with a Qubit^® DNA Assay Kit in Qubit^® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and 1.0 μg of DNA was used for library preparation. Genomic DNA was sonicated to generate 180–250 bp fragments and then end-polished, A-tailed, and ligated with the full-length adapter with further PCR amplification. PCR products were purified using AMPure XP system (Beckman Coulter, Brea, CA, USA) and quantified using the Agilent high sensitivity DNA assay on the Agilent Bioanalyzer 2100 system. The whole exome was captured with Agilent SureSelect Human All Exome V6 (Agilent Tecnologies, Santa Clara, CA, USA), and the sequencing was performed on an Illumina NovaSeq 6000 Systems.