Masterplex qt analysis software

Serum cytokine levels were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following the manufacturer's instructions. Bio-Plex Pro Human Th17 Cytokine Panel, Bio-Plex Pro Human Cytokine 27-plex Panel, and Bio-Plex Human Cytokine 21-plex Panel were used for detection of a total of 57 analytes. Serum aliquots (50 μL) were collected from healthy donors and 42 MS cases. A minimum of 50 beads per analyte was acquired. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected was analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by manufacturer.

Supernatant cytokine profiles were analyzed using Bio-Plex Pro Human Cytokine 27-plex Panel and Bio-Plex Human Cytokine 21-plex Panel (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits, following the manufacturer’s instructions. Supernatant aliquots (50 µL) were used for analysis, with a minimum of 50 beads per analyte acquired. Median fluorescence intensities were measured using a Bioplex 200 (Bio-Rad, Hercules, CA, USA) analyzer. Data collected were analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by the manufacturer.

Serum and CSF cytokine profiles were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following the manufacturer's instructions. Bio-Plex Pro Human Cytokine Screening Panel, 48-Plex was used for detection for a total of 48 analytes. Serum and CSF aliquots (50 μl) were used for analysis, with a minimum of 50 beads per analyte acquired. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected was analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by the manufacturer.
Data on forty-eight cytokine levels (IL-1α, IL-1β, IL-1Ra, IL-2, IL-2Ra, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12(p70), IL-13, IL-16, IL-15, IL-17, IL-18, CCL2, CCL3, CCL4, CCL5, CCL7, CCL11, CCL27, CXCL1, CXCL9, CXCL10, FGF basic, G-CSF, GM-CSF, HGF, IFNα2, INF-γ, LIF, M-CSF, MIF, β-NGF, PDGF-bb, SDF-1a, SCF, SCGF-b, TNF-α, TNF-β, TRAIL, and VEGF) in serum (101 MS patients and 101 non-MS controls) and CSF (101 MS patients and 25 non-MS controls) was analyzed. Data on three cytokines (IL- 1Ra, IL-2ra, and IL-17) was found to be missing in some patients; therefore, these three cytokines were excluded from the dataset used in building the machine learning models.

Serum and CSF cytokine profiles were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following the manufacturer’s instructions. Bio-Plex Pro Human Cytokine 27-plex Panel and Bio-Plex Human Cytokine 21-plex Panel were used for detection for a total of 48 analytes. Serum and CSF aliquots (50 µl) were used for analysis, with a minimum of 50 beads per analyte acquired. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected were analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by the manufacturer.

Serum cytokine levels were analyzed using single-plex sets for IL-1b, IL-2, IL-5, IL-4, IL-6, IL-8, IL-10, IL-12p40, GM-CSF, IFN-γ, CXCL10, and TNF-α (Bio-Rad, Hercules, CA, USA) following the manufacturer's instructions. Serum aliquots (50 μL) were used for analysis, with a minimum of 50 beads acquired per analyte. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected was analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by the manufacturer.