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2 protocols using rpmi 1640 culture medium

1

Investigating Docetaxel Resistance in Gastric Cancer

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Human gastric cancer SGC7901 cells and drug-resistant SGC7901/Docetaxel cells were kindly donated by the Basic Medical College of Zhengzhou University. Thirty female nude mice 4 weeks old, weighing 10–15 g, were provided by the Animal Experimental Center of Zhengzhou University. RPMI-1640 culture medium and fetal bovine serum (FBS) were purchased from Zhejiang Tianhang Biotechnology Co., Ltd. (Hangzhou, China). Lipofectamine 2000 Transfection kit was purchased from Invitrogen Co. (Carlsbad, CA, USA). Docetaxel and MTT kits were provided by Sigma-Aldrich Co. (St. Louis, MO, USA). Annexin V/PI Apoptosis Detection kit and Protein Extraction kit were from Beyotime Biotechnology (Shanghai, China). STMN-1 (MDR1 or ERCC1 or caspase 3) mouse anti-human monoclonal antibody and rabbit anti-mouse antibody second antibody were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
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2

Folate-Targeted Graphene Oxide Nanocarrier

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Multilayer Graphene flake was obtained from Hengqiu Tech Inc. Potassium permanganate (KMnO4, 99%), hydrochloric acid (HCl, 37%), sulfuric acid (H2SO4, 98%), hydrogen peroxide (H2O2, 30%), and anhydrous chloroform were obtained from Tianjin Damao Chemical Reagent Co. China. Doxorubicin hydrochloride (DOX) was purchased from Shanghai Yijing Industrial Co. SPC and the FA containing polyethylene glycol (PEG) distearoylphosphatidylethanolamine (DSPE-PEG2000-Folate) were bought from Ponsure Biotechnology Co. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), were purchased from Shanghai EKEAR Bio@Tech Co. LTD. LysoTracker-Red and Hoechst 33342 were obtained from Beyotime Biotechnology Co. Penicillin, streptomycin, RPMI 1640 culture medium, and fetal bovine serum (FBS) were obtained from Zhejiang Tianhang Biotechnology Co. Ltd. Double distilled water was obtained from Milli-Q system of Millipore Co., USA. Chlorpromazine (CPZ), methyl-β-cyclodextrin (MCD), and amiloride (AMI) were bought from Sangon Biotech (shanghai) Co., Ltd. Sodium dihydrogen phosphate, methanol and glacial acetic acid were purchased from Shanghai MACKLIN Chemical Reagent Co., LTD.
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