PTX-loaded liposomes (Lip/PTX) were prepared by thin film dispersion method. Briefly, 8.6mg DPPC, 8.95mg DSPE-PEG2000, 2.47mg cholesterol (the mole ratio of DSPE-PEG2000:DPPC:Cholesterol was 15:55:30 and the content of DSPE-PEG2000 was 15%) and PTX (0.5mg) were dissolved in 2mL of chloroform at 45°C. Evaporation in vacuum state removed the chloroform to form a film, which was continued to be evaporated for 1h after the solution dried. At 45°C, 2mL RO water was used to hydrate the film for 30min, and the PTX liposome was extruded through 200nm polycarbonate membrane for three times by liposome extruder (Avestin, Canada).
Liposome extruder
Manufactured by Avestin
Sourced in Canada
The Liposome Extruder is a laboratory equipment used to produce uniform liposome vesicles. It works by forcing a lipid suspension through a polycarbonate membrane with defined pore sizes, resulting in the extrusion of liposomes with a homogeneous size distribution.
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Lab products found in correlation
2 protocols using liposome extruder
1
Formulation and Characterization of PTX-loaded Liposomes
2
Liposome Preparation Protocol
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An established protocol25 (link) was used to prepare liposomes. Briefly, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) procured from Avanti Polar Lipids25 (link) and β-sitosterol or cholesterol were mixed at a molar ratio of 45:55 in chloroform. The solvent was removed with a stream of argon or nitrogen at 40 °C, and the mixture was further dried under vacuum for 3 hours. 500-μl of the mixture was added to the dried lipids and the suspension was mixed by vortexing and sonication in a water bath for 5 min to ensure that the lipid was fully hydrated. The suspended mixture was then passed 21 times in 0.5 mL increments through an Avestin Inc (Ottawa, ON) liposome extruder to generate liposomes. The liposomes were stored at 4 °C and used within 5 days of preparation.
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