Strap

Manufactured by BD

STRAP is a laboratory equipment product designed for securing and holding various laboratory items in place. It provides a secure and adjustable solution for positioning and stabilizing equipment, instruments, or samples during experiments or procedures.

Automatically generated - may contain errors

Lab products found in correlation

Cleaved notch1, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by BD (1 mentions) Sr140, by Santa Cruz Biotechnology (1 mentions) Sf3a2, by Santa Cruz Biotechnology (1 mentions) Sf3b1, by Santa Cruz Biotechnology (1 mentions) Snrpa, by Santa Cruz Biotechnology (1 mentions) Tfiiic110, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using strap

Protein Interaction and Expression Analysis

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Co-IP and western blot assays were performed as described previously (18 (link)). Primary antibodies included: CD133 (18470-1-AP) (Proteintech Group); ABCG2 (sc-25822), HA (sc-805) (Santa Cruz Biotechnology); CD44 (#558739) and STRAP (#611346) (BD Transduction Labs); Cleaved Caspase 3 (#9661), Cleaved NOTCH1 (#4147), DLL1 (#2588), DLL4 (#2589), NUMB (#2756), TACE (#6978), JAG1 (#2620), JAG2 (#2210), HES1 (#11988), OCT4 (#2840), SNAIL (#3879), SLUG (#9585) and BMI1 (#5856) (Cell Signaling Technology); Tri-Methyl-Histone H3 (Lys27) (ab192985), SUZ12 (ab12073), EZH2 (ab3748), EED (ab169647) (Abcam); and β-actin (A5316) and Flag (F3165) (Sigma). More details are in Supplementary Information.

Jin L., Vu T., Yuan G, & Datta P.K. (2017). STRAP promotes stemness of human colorectal cancer via epigenetic regulation of the NOTCH pathway. Cancer research, 77(20), 5464-5478.

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Western Blot Profiling of Spliceosomal Proteins

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Proteins were separated by 10% SDS-PAGE and probed with primary antibodies. Primary antibodies, at the dilution of 1:1000 for each, included: SNRPA (Santa Cruz, sc-376027); U2A’ (Santa Cruz, sc-393804); SR140 (Santa Cruz, sc-398718); HELIC2 (Santa Cruz, sc-393170); PRPF3 (Santa Cruz, sc-101130); SYF1 (Santa Cruz, sc-271037); SF3A3 (Santa Cruz, sc-393673); SF3A2 (Santa Cruz, sc-390444); SF3B1 (Santa Cruz, sc-514655); STRAP (BD Transduction Labs, #611346); DDX15 (Santa Cruz, sc-271686); TFIIIC110 (Santa Cruz, sc-81406); GAPDH (Cell Signaling, #2118), and CHERP (Santa Cruz, sc-100650). β-Actin (Sigma, A5316) was used at the dilution of 1:10,000.

Jin L., Chen Y., Crossman D.K., Datta A., Vu T., Mobley J.A., Basu M.K., Scarduzio M., Wang H., Chang C, & Datta P.K. (2020). STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature Communications, 11, 5941.

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