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Hbsag

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HBsAg is a laboratory test kit used to detect the hepatitis B surface antigen (HBsAg) in human blood samples. It is a fundamental diagnostic tool for identifying hepatitis B virus (HBV) infection.

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Lab products found in correlation

Np18 ova, by Biosearch Technologies (1 mentions)

2 protocols using hbsag

1

Immunization Protocol with NP-OVA and HBsAg

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Mice were injected i.p. with 200 µl of a suspension containing 50% volume of immunogen solution and 50% volume of alum (Thermo Fisher Scientific; catalog no. 77161). The immunogens were: NP(18)-OVA (Biosearch Technologies; catalog no. N-5051-100) and recombinant hepatitis surface antigen (HBsAg; ProSpec; catalog no. HBS-872-3). The dose of immunogen was 10 µg per mouse, unless otherwise indicated. For immunohistology of GCs in spleen, SRBCs (Innovative Research; IC10-0210-15ML) were washed and resuspended in PBS and then injected i.p. at 250 × 106 cells per mouse (in 0.2 ml).
Zhong M.C., Lu Y., Qian J., Zhu Y., Dong L., Zahn A., Di Noia J.M., Karo-Atar D., King I.L, & Veillette A. (2020). SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunity. The Journal of Experimental Medicine, 218(3), e20200756.
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2

Immunization with Low Endotoxin OVA

Cited 1 time
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Low endotoxin grade OVA (<0.01 EU/μg protein) was used for immunization (Hyglos). CpG type-B 1826 oligonucleotide (5′-TCCATGACGTTCCTGACGTT-3′) was purchased from Microsynth AG and HBsAg from Prospec-Tany TechnoGene Ltd. FNIII 11-EDA was produced and purified as described by Julier et al. [6] (link).
Julier Z., de Titta A., Grimm A.J., Simeoni E., Swartz M.A, & Hubbell J.A. (2016). Fibronectin EDA and CpG synergize to enhance antigen-specific Th1 and cytotoxic responses. Vaccine, 34(21), 2453-2459.
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