Reference strains of methicillin-resistant Staphylococcus aureus (MRSA) used in this study were ATCC 33591, ATCC 43300 and ATCC 700699 purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Two clinical MRSA strains (BD 15358 and BD 16876) used in the assays were obtained from the Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur. All test bacteria were cultured overnight to a logarithmic phase of growth at 37 °C in Mueller-Hinton broth (MHB; Merck, NJ, USA) medium prior to the assays. Bacterial suspension from overnight cultures was adjusted using MHB medium to an optical density (OD) at λ 625 nm of 0.08–0.13, determined using a spectrophotometer, which corresponds to 106 colony forming units (CFU)/mL. For culture stock maintenance, strains were grown on Mueller-Hinton agar (MHA; Merck, NJ, USA) and cell suspensions prepared in 20 % (v/v) glycerol were stored at −80 °C.
Atcc 700699
Manufactured by American Type Culture Collection
Sourced in United States
ATCC 700699 is a microbial culture medium that supports the growth of a wide range of microorganisms, including bacteria, yeast, and fungi. It is composed of a mixture of nutrients, vitamins, and growth factors that provide the necessary components for the cultivation of diverse microbial species.
Automatically generated - may contain errors
Lab products found in correlation
Mueller hinton broth (mhb), by Merck Group (1 mentions)
Mueller hinton agar (mha), by Merck Group (1 mentions)
Atcc 43300, by American Type Culture Collection (1 mentions)
Atcc 33591, by American Type Culture Collection (1 mentions)
Luria bertani broth lb, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin fungizone, by Lonza (1 mentions)
Tryptic soy broth tsb, by Solabia (1 mentions)
Atcc 25923, by American Type Culture Collection (1 mentions)
Foetal bovine serum (fbs), by Biowest (1 mentions)
Atcc 10145, by American Type Culture Collection (1 mentions)
2 protocols using atcc 700699
1
MRSA Reference Strain Preparation
2
Antimicrobial and Biofilm Assays
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Pseudomanas aeruginosa, VRSA, MSSA and MRSE strains were obtained from the American Type Culture Collection (ATCC 700699, ATCC10145, ATCC 25,923 and ATCC 51,625, respectively; Manassas, VA, USA). A. baumannii and MRSA strains were obtained from the culture collection of the Göteburg University (CCUG; Gothenburg, Sweden) (CCUG 61,012 and CCUG 60578). S. epidermidis was obtained from the Belgian Co-ordinated Collections of Microorganisms (Belgium) (LMG 10474; Gent, Belgium).
For antimicrobial testing inoculums were prepared in Luria-Bertani Broth (LB; ThermoFisher Scientific, Waltham, MA, USA) and for biofilms assays in Tryptic Soy Broth (TSB, Biokar Diagnostics, Beauvais, France).
For cell-based assays human keratinocytes (HaCat) were obtained from Cell Line Services (Oppenheim, Denmark). For assays cells were cultured, at 37 °C in a humidified atmosphere of 95% air and 5% CO2, using Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose, L-glutamine without pyruvate (Lonza, Verviers, Belgium) containing 10% foetal bovine serum (FBS, Biowest, Nuaillé, France) and 1% (v/v) Penicillin-Streptomycin-Fungizone (Lonza, Verviers, Belgium).
For antimicrobial testing inoculums were prepared in Luria-Bertani Broth (LB; ThermoFisher Scientific, Waltham, MA, USA) and for biofilms assays in Tryptic Soy Broth (TSB, Biokar Diagnostics, Beauvais, France).
For cell-based assays human keratinocytes (HaCat) were obtained from Cell Line Services (Oppenheim, Denmark). For assays cells were cultured, at 37 °C in a humidified atmosphere of 95% air and 5% CO2, using Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose, L-glutamine without pyruvate (Lonza, Verviers, Belgium) containing 10% foetal bovine serum (FBS, Biowest, Nuaillé, France) and 1% (v/v) Penicillin-Streptomycin-Fungizone (Lonza, Verviers, Belgium).
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