Ly2811376

LY2886721, LY2811376, AZD3293, AZD3839, MK-8931, Pepstatin A, TAPI-1, Batimastat and DAPT were from Selleckchem; E2609 was from Sun-Shine Chemical; PF-06751979 was from MedChemExpree; β-secretase inhibitor IV was from Millipore; GI 254023X was from TOCRIS; Roburic acid was from Aobious; synthetic β-amyloid peptides are all from Anasepc; and recombinant human BACEl and BACE2 protein was from R&D system.

The human H4 neuroglioma cell line was purchased from ATCC (Manassas, VA). Dulbecco’s Modified Eagle Medium (DMEM), Penicillin-Streptomycin and culture dishes were from Corning Life Sciences (Tewksbury MA); Fetal bovine serum (Premium Select) was purchased from Atlanta Biologicals (Flowery Branch, GA); DC Protein Assay Kit, Pre-stained Dual Color Protein Standards, Clarity Western ECL, Chemidoc MP System, Image Lab Software, Chemi Hi Sensitivity blot application, Immuno-Blot LF PVDF membrane, 4–20% Mini Protean TGX Stain Free precast gels, and 4–15% Mini Protean TGX Stain Free precast gels were obtained from BioRad (Hercules, CA); polyvinylidene fluoride (PVDF) transfer membrane (0.45mm) was purchased from Perkin Elmer (Waltham, MA); Whatman nitro-cellulose transfer membrane (0.2 μm) was purchased from GE Healthcare Life Sciences; Lipofectamine 2000, Pierce ECL Western Blotting, Pierce SuperSignal West Pico substrates and G418 sulfate (Geneticin) were all from ThermoFisher Scientific (Waltham, MA); Recombinant Human BACE1 protein, CF (931-AS) and Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate (ES004) were acquired from R&D Systems (Minneapolis, MN); the BACE1 inhibitor LY2811376 was purchased from Selleckchem (Houston, TX).