30μg of protein lysates prepared from hemibrains homogenized in RIPA buffer were loaded in Criterion XT 4-20% Bis-Tris gels and transferred onto PVDF membrane (0.45μm; Millipore, Billerica, MA, USA). Membranes were probed with anti-C1q antibody (1:1000, cat#ab182451, Abcam) and subsequently incubated with anti-rabbit HRP-conjugated secondary antibodies (1:2000, cat#PI-1000, Vector laboratories). Normalization was achieved using GAPDH. Membranes were probed with anti-GAPDH antibody (1:5000, cat#sc32233, Cruz Biotechnology, Dallas, TX, USA) and subsequently incubated with anti-mouse HRP-conjugated secondary antibodies (1:2000, cat#PI-2000, Vector laboratories). Membranes were developed with ECL Western blotting substrate (Pierce, Rockford, IL, USA) using the Fujifilm LAS-3000 developer (Stamford, CT, USA). Integrated density of immunoreactive bands were measured using MultiGauge Software (FujiFilm). (see suppl. methods for details)
Anti rabbit hrp conjugated secondary antibodies
Manufactured by Vector Laboratories
Anti-rabbit HRP-conjugated secondary antibodies are laboratory reagents used to detect and visualize the presence of rabbit primary antibodies in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry. These secondary antibodies are conjugated with the enzyme horseradish peroxidase (HRP), which can catalyze a colorimetric or chemiluminescent reaction, allowing for the specific detection of the target rabbit primary antibody.
Automatically generated - may contain errors
Lab products found in correlation
Anti c1q antibody, by Abcam (2 mentions)
Anti mouse hrp conjugated secondary antibody, by Vector Laboratories (2 mentions)
Multi gauge software, by Fujifilm (2 mentions)
Pvdf membrane 0.45μm, by Merck Group (1 mentions)
Las 3000 developer, by Fujifilm (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
2 protocols using anti rabbit hrp conjugated secondary antibodies
1
Western Blot Analysis of C1q Protein
2
Western Blot Analysis of C1q Protein
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30 µg of protein lysates prepared from hemibrains homogenized in RIPA buffer were loaded in Criterion XT 4-20% Bis–Tris gels and transferred onto PVDF membrane (0.45 μm; Millipore, Billerica, MA, USA). Membranes were probed with anti-C1q antibody (1:1000, cat#ab182451, Abcam) and subsequently incubated with anti-rabbit HRP-conjugated secondary antibodies (1:2000, cat#PI-1000, Vector laboratories). Normalization was achieved using GAPDH. Membranes were probed with anti-GAPDH antibody (1:5000, cat#sc32233, Cruz Biotechnology, Dallas, TX, USA) and subsequently incubated with anti-mouse HRP-conjugated secondary antibodies (1:2000, cat#PI-2000, Vector laboratories). Membranes were developed with ECL Western blotting substrate (Pierce, Rockford, IL, USA) using the Fujifilm LAS-3000 developer (Stamford, CT, USA). Integrated density of immunoreactive bands were measured using MultiGauge Software (FujiFilm) (see Suppl. methods for details).
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