Systolic (SBP) and mean arterial blood pressure (MABP) values were measured by tail-cuff method using a non-invasive indirect blood pressure system (MAY NIBP-200A, Commat Ltd Inc., Ankara, Turkey), which is compatible with BIOPAC MP100 Data Acquisition System (Biopac System Inc., Santa Barbara, CA, USA). The rats were first placed in an acrylic chamber (MAY Tail Heating-B, Commat Ltd Inc.) at 37°C for 20-30 min with an acrylic restrainer and then transferred to a standard setup with tail cuff and infrared sensor (RXTCUFSENSOR11, Biopac System Inc.), which is compatible with NIBP-200A. The cuff with infrared sensor was placed on the rat’s tail immediately after heating. SBP and MABP values were recorded with BIOPAC Acknowledge Software (V3.5.7, Biopac System Inc.) and the average value of at least five consecutive readings was obtained for each rat. Rats had been allowed to accustom to this procedure for 7 days before experiments were performed. All measurements were performed by the same person, who was blinded to the purpose of the study. SBP and MABP measurements were performed at the baseline, 4th, and 7th week.
Mp100 data acquisition system
Manufactured by Biopac
Sourced in United States
The MP100 data acquisition system is a versatile and reliable tool for recording and analyzing physiological signals. It is designed to capture a wide range of data, including electrical activity, pressure, and other measurements, with high accuracy and precision. The system offers a range of connectivity options and can be easily integrated into various research and clinical settings.
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9 protocols using mp100 data acquisition system
1
Non-invasive Blood Pressure Monitoring
2
Measuring Right Ventricular Systolic Pressure in Rats
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After 4 weeks of the single MCT injections, including 2 FMN injections, all rats were weighed and then anesthetized with pentobarbital sodium (50 mg/kg). Subsequently, RVSP was measured as reported in our previous study (25 (link)). In brief, after the rat was anesthetized, the right neck tissue was cut open and the right external jugular vein was isolated, then the venous catheter was inserted into the right ventricle from the right external jugular vein. Significant amplitude of right ventricular wave was observed, and RVSP was recorded using a miniature pressure transducer (cat. no. TSD104A; BIOPAC Systems, Inc.) and analyzed using a BIOPAC MP100 data acquisition system (BIOPAC Systems, Inc.).
3
Carnosine's Impact on Septic Shock
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Eight rats received laparotomy and CLP. After a further 60 min, a dose of 250 mg/kg carnosine diluted into 5 mL saline was injected. Hemodynamic responses were recorded simultaneously. Arterial pressure was measured using the Transpac® IV Disposable Pressure Transducer (ICU Medical Inc., USA). Invasive arterial blood pressure and pulse rate were monitored using an MP100 data acquisition system (Biopac, USA).
A MAP of <60 mm/Hg was used to define septic shock. The rats received an injection of 10 mL/kg saline when sepsis developed. At 24 h after CLP, blood samples were collected, as in Group 1 and Group 2. Rats were sacrificed 24 hours following laparotomy and the CLP procedure using an overdose of ketamine. Kidney tissue specimens were taken for MDA measurement and histopathology examination.
A MAP of <60 mm/Hg was used to define septic shock. The rats received an injection of 10 mL/kg saline when sepsis developed. At 24 h after CLP, blood samples were collected, as in Group 1 and Group 2. Rats were sacrificed 24 hours following laparotomy and the CLP procedure using an overdose of ketamine. Kidney tissue specimens were taken for MDA measurement and histopathology examination.
4
Continuous Arterial Blood Pressure Monitoring
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Mean arterial blood pressure (MAP) was continuously monitored during the experimental period through the arterial catheter and a pressure transducer. Analog pressure signals were digitized (MP100 Data Acquisition System, BIOPAC Systems, Goleta, CA, USA) and processed using data acquisition software for hemodynamic experiments (AcqKnowledge Software v. 3.5.7, BIOPAC Systems, Goleta, CA, USA). Heart rate (HR) was determined from the pressure trace and expressed as beats per minute (bpm).
5
Thermal Responses of Crustacean Cardiorespiration
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Heart and gill ventilation rates in P. monodon (n = 8) and A. astacus (n = 9) were measured in individual animals using a total of 16 (body mass = 87.9 ± 5.2 g) and 18 (body mass = 85.3 ± 4.4 g) animals, respectively. Heart and gill ventilation rates were measured using reflective infrared sensors (AMP03, Newshift Ltd, Leiria, Portugal) fitted into plastic tubes and glued onto the carapace above either the heart or the scaphognathites. The animal thus instrumented was submerged at 30 ± 0.1 °C (P. monodon) or 18 ± 0.1 °C (A. astacus) in fully oxygenated water and left overnight to obtain resting values. The following morning, water temperature was elevated linearly at 2 °C h−1, while heart or ventilation rates were recorded using Biopac MP100 data acquisition system at 200 Hz (12 bit).
6
Continuous Arterial Pressure Monitoring
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Mean arterial blood pressure (MAP) was continuously monitored during the experimental period through the arterial catheter connected to a pressure transducer. Analog pressure signals were digitized (MP100 Data Acquisition System, BIOPAC Systems, Goleta, CA, USA) and processed using data acquisition software for hemodynamic experiments (AcqKnowledge Software v. 3.5.7, BIOPAC Systems, Goleta, CA, USA). Heart rate (HR) was determined from the pressure trace and expressed as beats per minute (bpm).
7
Measuring Cavernous Nerve Stimulation and Intracavernosal Pressure
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To measure ICP with cavernous nerve stimulation, animals were anesthetized by intraperitoneal injection of 40 mg/kg pentobarbital sodium. The right carotid artery was cannulated with polyethylene tubing containing heparinized saline for continuous monitoring of mean arterial pressure (MAP). The shaft of the penis was then exposed from skin and muscle and punctured with a 23-gauge needle connected to the tubing. Both were connected to a pressure transducer (Biopac system Inc, Goleta, CA, USA). The cavernous nerve was stimulated using a bipolar electrode connected to a stimulator. Stimulator settings were 5 V of 1.5 mA for 1 min with a minimum interval between stimulation of 5 min. The ICP/MAP ratio was determined using the maximum ICP divided by the MAP obtained during cavernous nerve stimulation. All data were recorded by an MP100 data acquisition system and analyzed using Acqknowledge software (Biopac System Inc., Goleta, CA, USA).
8
Heart Rate Variability Analysis Protocol
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HRV was recorded and analyzed as per the recommendation of Task Force on HRV [24 (link)] and as practiced in our AFT Lab [23 (link)], by continuous ECG recordings for 10 min using BIOPAC MP-100 data-acquisition system and Acknowledge software version 3.8.2 (BIOPAC Inc, USA). Time-domain indices of HRV such as SDNN and RMSSD, and frequency-domain indices of HRV such as total power (TP), low-frequency (LF) and high-frequency (HF) power and LF/HF ratio were calculated by the software.
9
Bladder Function Assessment in Rats
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Four weeks following injections, i.e. on the night before sacrifice, the animals in the i.p. injected groups (Saline i.p. and Desipramine i.p.) were individually housed for 8 h (dark cycle, from 9 PM to 5 AM) with free access to water, but no food, in metabolic cages fitted with a doppler sensor (SICK, Stockholm, Sweden) connected to a MP100 data acquisition system (Biopac Systems Inc., Goleta, CA). The bladder function was monitored and parameters including total number of micturitions, inter-micturition intervals and number of drops per micturition were registered using Acknowledge Software v 3.8 (Biopac Systems Inc.). The total urine volume, number and weight of feces pellets and total water consumed was also registered.
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