Adiponectin total elisa kit

Blood samples were centrifuged immediately after they were obtained, and the supernatant was stored at -80° C. The measurement of all hormones took place at the same point of time to avoid inter-assay variabilities. Blood serum and EDTA (Ethylenediaminetetraacetic acid) plasma were used to measure the hormone levels through an immunoassay. The ACTH-assay (Roche Diagnostics, ELCIA, Indianapolis, IN, USA) had a measuring range of 0.220–440 pmol/L, an intra-assay coefficient of variation (CV) of < 2.4% and an inter-assay CV of < 4.2%. The C-peptide-assay had a measuring range of 0.003–13.3 nmol/L, an intra-assay CV of <4.6% and an inter-assay CV of < 5.0. The Insulin-assay had a measuring range of 1.39–6945 pmol/L, an intra-assay CV of < 2.8% and an inter-assay CV of < 4,9%. Cortisol had a measuring range of 0.5–1750 nmol/L, an intra-assay CV of < 2.9% and an inter-assay CV of < 4.7%. Adiponectin levels were measured with an Adiponectin ELISA (Immundiagnostik AG, Adiponectin total ELISA Kit, Bensheim, Germany) with an intra-assay CV of < 3.4% and inter-assay CV of < 6.3%.