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Biospin gmbh spectrometer

Manufactured by Bruker
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Sourced in Germany

The BioSpin GmbH spectrometer is a laboratory equipment designed for spectroscopic analysis. It is capable of performing nuclear magnetic resonance (NMR) spectroscopy, a technique used to study the structure and properties of molecules. The spectrometer's core function is to generate and detect electromagnetic radiation, which interacts with the nuclei of atoms in the sample, providing information about the sample's chemical composition and molecular structure.

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Lab products found in correlation

Silica gel, by Qingdao Haiyang Chemical (3 mentions) Lcms 2020, by Shimadzu (1 mentions) Lcms it tof mass spectrometer, by Shimadzu (1 mentions) Agilent 1100 lc msd trap sl, by Agilent Technologies (1 mentions)

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BioSpin spectrometer Bruker spectrometers

3 protocols using biospin gmbh spectrometer

1

Characterization of Small Organic Molecules

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1H NMR and 13C NMR spectra were recorded using TMS as the internal standard in DMSO-d6 or CDCl3 with a Bruker BioSpin GmbH spectrometer. The mass spectra (MS) were recorded on a Thermo Scientific LTQ ORBITRAP instrument with an ESI mass selective detector. Melting points (m.p.) were determined using an SRS-OptiMelt automated melting point instrument, without correction. Flash column chromatography was performed with silica gel (200–300 mesh) purchased from Qingdao Haiyang Chemical Co., Ltd.
Xue S., Ma L., Gao R., Li Y, & Li Z. (2014). Synthesis and antiviral activity of some novel indole-2-carboxylate derivatives. Acta Pharmaceutica Sinica. B, 4(4), 313-321.
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2

NMR and HPLC Analysis of Synthesized Compounds

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All chemicals were purchased from local commercial suppliers and used without further purification unless otherwise specified. All the solvents were of an analytical reagent grade. Nuclear magnetic resonance spectra were recorded on a Bruker BioSpin GmbH spectrometer at 400 or 500 MHz using tetramethylsilane (TMS) as the internal reference. Melting points were determined in slides on an X-5 micro melting point apparatus without being corrected. Mass spectra were recorded on a Shimadzu LCMS-2020 instrument with an electrospray ionization (ESI) mass selective detector, and HRMS were recorded on a Shimadzu LCMS-IT-TOF mass spectrometer. The synthesized compounds were purified through column chromatography with silica gel (200–300 mesh) purchased from Qingdao Haiyang Chemical Co. Ltd. All compounds tested for biological activities were analyzed by high-performance liquid chromatography (HPLC), and their purities were more than 95%. The analysis condition was detection at 220 nm, 1.0 mL/min flow rate, and a linear gradient from 55% to 15% phosphatebuffered solution (PBS) buffer (pH 3) and 45%–85% methanol (MeOH) in 30 min.
Pelttari J., Hoja M.R., Yuan L., Liu J.G., Brundell E., Moens P., Santucci-Darmanin S., Jessberger R., Barbero J.L., Heyting C, & Höög C. (2001). A Meiotic Chromosomal Core Consisting of Cohesin Complex Proteins Recruits DNA Recombination Proteins and Promotes Synapsis in the Absence of an Axial Element in Mammalian Meiotic Cells. Molecular and Cellular Biology, 21(16), 5667-5677.
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3

NMR and Mass Spectrometry Analysis

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All reagents were chemically pure or analytically pure products and were not further purified. The 1H NMR and 13C NMR spectra were measured by a Bruker BioSpin GmbH spectrometer (Bruker Company, Karlsruhe, Germany) at 300 and 75 MHz, respectively. DMSO-d6, MeOD, or CDCl3 were used as solvents. TMS was used as the internal standard, the chemical shift unit was ppm, and the coupling constant unit was Hz. Proton coupling modes were described as singlet (s), broad singlet (brs), doublet (d), doublet of doublets (dd), triplet (t), and multiplet (m). The mass spectrometry (MS, HRMS) was determined by an Agilent 1100-LC-MSD-Trap/SL or an FTMS-2000 mass spectrometer (Agilent Technologies Inc., Santa Clara, CA, USA).
Wu X., Ze X., Qin S., Zhang B., Li X., Gong Q., Zhang H., Zhu Z, & Xu J. (2024). Design, Synthesis, and Biological Evaluation of Novel Tetrahydroacridin Hybrids with Sulfur-Inserted Linkers as Potential Multitarget Agents for Alzheimer’s Disease. Molecules, 29(8), 1782.
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