Goat anti calretinin

Papain was from Roche (Mannheim, Germany). All other substances used were from Sigma-Aldrich (Taufkirchen, Germany), unless stated otherwise. The following primary antibodies were used: rabbit anti-Kir4.1 (1 : 200; Sigma-Aldrich), mouse anti-GFAP (1 : 200; G-A-5 clone, Sigma-Aldrich), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1; 1 : 500, Wako, Neuss, Germany), goat anti-calretinin (1 : 500, Swant, Marly, Switzerland), mouse anti-calbindin (1 : 400, Swant), rabbit anti-PKCα (1 : 300, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CRALBP (1 : 300, Santa Cruz Biotechnology) and mouse anti-glutamine synthetase (1 : 1000, Merck Millipore, Darmstadt, Germany). The following secondary antibodies were used: Cy5-conjugated donkey anti-goat, Cy3-conjugated donkey anti-rabbit, Cy2-conjugated donkey anti-mouse, Cy3-conjugated goat anti-rabbit and Cy2-conjugated goat anti-mouse. All secondary antibodies were applied in a 1 : 200 dilution and were obtained from Dianova (Hamburg, Germany). The apoptosis rate was detected using the in situ cell death detection kit, tetramethylrhodamine red (Roche).

All substances were purchased from Sigma‐Aldrich (Taufkirchen, Germany) unless stated otherwise. Papain was obtained from Roche (Mannheim, Germany). Chloromethyl‐tetramethyl‐rosamine (Mitotracker Orange), NP‐EGTA (o‐nitrophenyl EGTA), NPE‐ATP (P(3)‐[1‐(2‐nitrophenyl)]ethyl ester of ATP), and Fluo‐4 AM were from Molecular Probes (Life Technologies, Carlsbad, CA). For immunocyto‐ and histochemical staining, the following primary antibodies were used: rabbit anti‐Kir4.1 (1:200; Sigma‐Aldrich), mouse anti‐glial fibrillary acidic protein (GFAP; 1:200; G‐A‐5 clone, Sigma‐Aldrich), goat anti‐calretinin (1:500, Swant, Marly, Switzerland), mouse anti‐calbindin (1:400, Swant), rabbit anti‐PKCα (1:300, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti‐cellular retinaldehyde‐binding protein (CRALBP; 1:300, Santa Cruz), rabbit‐anti‐Iba1 (1:500, Wako Chemicals, Neuss, Germany), and mouse anti‐glutamine synthetase (1:1000, Merck Millipore, Darmstadt, Germany). As secondary antibodies, we used Cy5‐conjugated donkey anti‐goat, Cy3‐conjugated donkey anti‐rabbit, Cy2‐conjugated donkey anti‐mouse, Cy3‐conjugated goat anti‐rabbit, and Cy2‐conjugated goat anti‐mouse. All secondary antibodies were obtained from Dianova (Hamburg, Germany) and applied at 1:200 dilution. Apoptosis was detected by the in situ cell death detection kit (Roche, Mannheim, Germany).

All substances were purchased from Sigma-Aldrich (Taufkirchen, Germany) unless stated otherwise. Papain was obtained from Roche (Mannheim, Germany). Chloromethyl-tetramethyl-rosamine (Mitotracker Orange) was purchased from Molecular Probes (Life Technologies, Carlsbad, CA, USA). For immunofluorescence staining, the following primary antibodies were used: mouse anti-glial fibrillary acidic protein (GFAP; 1:200; G-A-5 clone, Sigma), goat anti-calretinin (1:500, Swant, Marly, Switzerland), rabbit-anti-Aif1 (1:500, Wako Chemicals), mouse anti-glutamine synthetase (1:1000, Merck Millipore, Darmstadt, Germany), rabbit anti-TSPO (1:100, Abcam, Cambridge, UK), and rabbit anti-DBI (1:200, Sigma). As secondary antibodies, we used Cy5-conjugated donkey anti-goat, Cy3-conjugated donkey anti-rabbit, Cy2-conjugated donkey anti-mouse, Cy3-conjugated goat anti-rabbit, and Cy2-conjugated goat anti-mouse. All secondary antibodies were obtained from Dianova (Hamburg, Germany) and applied at 1:200 dilution.