Chemicals and cloning reagents (restriction endonucleases, T4 DNA ligase, and DNA polymerase) were purchased from Sigma-Aldrich (St. Louis, MO, United States) and New England Biolabs (Ipswich, MA, United States), respectively. Plasmid DNA isolation and DNA extraction were conducted using plasmid preparation kits (Promega, Madison, WI, United States). Oligonucleotides were commercially synthesized and sequenced by Genotech (Daejeon, South Korea).
Plasmid preparation kits
Manufactured by Promega
Sourced in United States
Plasmid preparation kits are laboratory equipment used to isolate and purify plasmids from bacterial cultures. They facilitate the extraction and separation of plasmid DNA from other cellular components.
Automatically generated - may contain errors
Lab products found in correlation
Dna polymerase, by Merck Group (1 mentions)
T4 dna ligase, by Merck Group (1 mentions)
Diversify pcr random mutagenesis kit, by Takara Bio (1 mentions)
Dna cloning kits, by New England Biolabs (1 mentions)
Quikchange 2 xl site directed mutagenesis kit, by Takara Bio (1 mentions)
Puc19 plasmid, by New England Biolabs (1 mentions)
E coli dh5α cells, by New England Biolabs (1 mentions)
Restriction endonuclease, by New England Biolabs (1 mentions)
2 protocols using plasmid preparation kits
1
Molecular Cloning and Plasmid Preparation
2
Molecular Cloning Protocol Using Commercial Kits
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All chemical reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA). Restriction endonucleases, DNA cloning kits, pUC19 plasmid, and E. coli DH5α cells were purchased from New England Biolabs (Ipswich, MA, USA). The Diversify PCR Random Mutagenesis kit and QuikChange II XL Site-Directed Mutagenesis kit were purchased from Clontech (Mountain View, CA, USA) and Agilent Technologies (San Diego, CA, USA), respectively. Plasmid DNA isolation and DNA extraction were conducted using plasmid preparation kits (Promega, Madison, WI, USA). Oligonucleotides (Supplementary Table 5 ) were synthesized and sequenced by Macrogen (Daejeon, Korea).
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