ICR mice were purchased from Jilin Changchun YISI Laboratory Animal Technology Company. Mouse C2C12 cells were purchased from Procell (Wuhan, China). Retinol (VitA, R7632, purity ≥ 98%) and Retinoic acid (RA, R2625, purity ≥ 95%) were purchased from Sigma (Kawasaki, Japan). Dimethyl sulfoxide (DMSO, ab146588, purity > 98%) was purchased from abcom (Waltham, MA, USA). VitA powder was dissolved in dimethyl sulfoxide DMSO and diluted to different concentrations to treat C2C12 cell. Fetal bovine serum (FBS002), horse serum (YT2515), penicillin and streptomycin (YT2515) were purchased from Biological Industries (Cromwell, CT, USA). High glucose DMEM medium (11965092) was purchased from Gibco (Canberra, Australia). RIPA lysis buffer (P0013C) and DAPI (C1005) were purchased from Beyotime (Shanghai, China). Hematoxylin-eosin staining kit (G1120) was purchased from Solarbio (Beijing, China). Anti-GAPDH (sc-365062), Anti-MyoG (sc-52903) and Anti-MyoD (sc-377460) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and Anti-RARα (A0370) was purchased from ABclonal (Wuhan, China). Goat anti-Rabbit Anti-IgG (A0208) was purchased from Beyotime (Shanghai, China).
High glucose dmem medium 11965092
Manufactured by Thermo Fisher Scientific
Sourced in Australia, United States
High glucose DMEM medium (11965092) is a cell culture medium formulation designed to support the growth and maintenance of a variety of mammalian cell types. It contains 4.5 g/L of D-glucose as the primary energy source.
Automatically generated - may contain errors
2 protocols using high glucose dmem medium 11965092
1
Retinol-Induced Myogenic Differentiation
2
Macrophage RAW264.7 cell culture protocol
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The mouse macrophage cell line RAW264.7 was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). High-glucose DMEM medium (11965092) and fetal bovine serum (10100147) were purchased from Gibco (Grand Island, USA). A total RNA extraction kit (BSC52M1) was obtained from Bori Biotech Co., Ltd. (Anhui, China). A reverse transcription kit (RR047A) and fluorescent quantitative PCR reagent (RR420A) were purchased from Takara (Kyoto, Japan). A cell Counting Kit (CCK-8, FC101-02) was obtained from TransGen Biotech Co., Ltd. (Beijing, China). CD80 (11-0801-82), CD86 (25-0862-82) were purchased from Thermo Fisher (MA, USA). Tetramethylrhodamine isothiocyanate (TRITC Dextran, T1162) was purchased from Merck (NY, USA). Azoxymethane (AOM, A5486) was purchased from Sigma (MO, USA). Dextran sulfate sodium (DSS, 216011090) was obtained from MP Biomedicals (CA, USA). An immunohistochemical antibody kit (97624T) was obtained from Cell Signaling Technology (MA, USA). TAK-242 (A3850), PDTC (B6422), KG-501 (B8380), SR11302 (A8185), and LY294002 (A8250) were purchased from APExBIO (Houston, USA).
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