Recombinant human TGF-β1 was obtained from R&D Systems (Minneapolis, MN). The following antibodies were used for the study: mouse anti-human TGF-β1 monoclonal antibody (mAb) (1 : 50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-human Smad2/3 polyclonal Ab (pAb) (1 : 100, R&D Systems, Minneapolis, MN, USA), goat anti-human p-SMAD2/3 (Ser 423/425) pAb (1 : 100, Santa Cruz Biotechnology), mouse anti-human SMAD4 mAb (1 : 50, Santa Cruz Biotechnology), rabbit anti-human Snail mAb (1 : 50, Cell Signaling Technology, Danvers, MA), mouse anti-human β-actin mAb clone AC-15 (1 : 100, Sigma-Aldrich, St. Louis, MO), mouse anti-human E-cadherin mAb (1 : 100, Dako, Santa Clara, CA, USA), mouse anti-human vimentin mAb (1 : 100, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-human collagen type I pAb (1 : 500, Thermo Fisher Scientific, Waltham, MA, USA).
Goat anti human p smad2 3 ser 423 425 pab
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Goat anti-human p-SMAD2/3 (Ser 423/425) pAb is a polyclonal antibody raised in goats against the phosphorylated form of SMAD2 and SMAD3 proteins in humans, with phosphorylation occurring at serine residues 423 and 425. This antibody is designed for the detection of the activated, phosphorylated state of these SMAD transcription factors.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human tgf β1 monoclonal antibody mab, by Santa Cruz Biotechnology (3 mentions)
Goat anti human smad2 3 polyclonal ab pab, by R&D Systems (3 mentions)
Mouse anti human β actin mab clone ac 15, by Merck Group (3 mentions)
Mouse anti human e cadherin mab, by Agilent Technologies (3 mentions)
Mouse anti human vimentin mab, by Thermo Fisher Scientific (3 mentions)
Recombinant human tgf β1, by R&D Systems (2 mentions)
Erk inhibitor u0126, by Merck Group (1 mentions)
Sb431542, by R&D Systems (1 mentions)
3 protocols using goat anti human p smad2 3 ser 423 425 pab
1
TGF-β1 Signaling Pathway Evaluation
2
TGF-β1 Signaling Pathway Analysis
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Recombinant human TGF-β1 was purchased from R&D Systems (Minneapolis, MN, USA). Mouse anti-human TGF-β1 monoclonal antibody (mAb) (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-human SMAD2/3 polyclonal Ab (pAb) (1:100, R&D Systems, Minneapolis, MN, USA), goat anti-humanp-SMAD2/3 (Ser 423/425) pAb (1:100, Santa Cruz Biotechnology), mouse anti-human β-actin mAb clone AC-15 (1:100, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-human E-cadherin mAb (1:100, Dako, Santa Clara, CA, USA), mouse anti-human vimentin mAb (1:100, Thermo Fisher Scientific, Waltham, MA, USA), human Follistatin-like 1/FSTL1 polyclonal Antibody (pAb) (1 μg/mL, R&D Systems) were used in the experimental procedure.
3
Interleukin-17 Signaling Pathway Modulation
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Recombinant human Interleukin-17 (IL-17) (50 ng/ml) was purchased from Sigma-Aldrich (St. Louis, MO). The following antibodies were used for the study: mouse antihuman TGF-β1 monoclonal antibody (mAb) (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat antihuman Smad2/3 polyclonal Ab (pAb) (1:100, R&D Systems, Minneapolis, MN, USA); goat anti-human p-Smad2/3 (Ser 423/425) pAb (1:100, Santa Cruz Biotechnology), mouse anti-human Erk1/Erk2 mAb or mouse anti-human phospho-Erk1/Erk2 mAb (both from R&D systems); mouse anti-human β-actin mAb clone AC-15 (1:100, Sigma-Aldrich, St. Louis, MO); mouse anti-human E-cadherin mAb (1:100, Dako, Santa Clara, CA, USA), mouse anti-humanvimentin mAb (1:100, Thermo Fisher Scientific, Waltham, MA, USA); TGF-β receptor type I kinase inhibitor SB-431542 (10 mM in DMSO) (R&D systems, Minneapolis, MN, USA); Erk inhibitor U0126 (Sigma-Aldrich) at 10 μM.
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