To confirm the functionality of the magnetic microparticles, Fourier-transform infrared spectroscopy (FTIR) was applied using an FTIR spectrophotometer (Cary 660 series; Agilent Technologies, Victoria, Australia), equipped with a deuterated triglycine sulfate detector in the scanning range of 400 to 4000 cm-1. FTIR provides an experimental and interpretive framework of the structure, interactions of natural polymer systems and physical characteristics (31 (link), 32 (link)). The surface charge and hydrodynamic size of the particles was measured using dynamic light scattering instrument (Malvern Instruments Ltd., Worcestershire, UK). The volume size distribution was calculated from the intensity of the light diffracted at each angle. Transmission electron microscopy (TEM; JEOL, Leoben, Austria) was also used to acquire images that show the surface morphology and structure of the particle solution. The effect of magnetic susceptibility and coating on the paramagnetic properties of synthesized particles was determined using vibrating sample magnetometer at room temperature (VSM, Kashan, Iran).
Cary 660 series
Manufactured by Agilent Technologies
Sourced in Australia
The Cary 660 series is a line of laboratory equipment manufactured by Agilent Technologies. The core function of this product is to perform spectroscopic analysis, allowing users to measure the absorption or transmission of light through samples across a range of wavelengths.
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Lab products found in correlation
3 protocols using cary 660 series
1
Characterization of Magnetic Microparticles
2
Quantifying Chemical Differences in Wheat Beers
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Samples were analyzed using an FTIR coupled with ATR analysis (Agilent model: Cary 660 Series) with 4000–600 cm−1 scale and a variation of 4 cm−1 in order to quantify the change in chemical structure. A blank ATR cell was utilized to measure the background of the samples. In order to compare black and white wheat beers, the absorbance strength of each spectrum was observed.
3
Analyzing Biomembrane Composition by ATR/FT-IR
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ATR/FT-IR spectra were recorded by using a Cary 660 series Agilent Technologies, between 650 e 4000 cm -1 , at 25 °C, 64 scans. The spectra were collected on the vesicular suspensions and plain medium (PB) was used as blank for spectral subtraction. At least, five spectra were collected for each sample.
The spectra were processed by using IGOR PRO 6.05 (Wave metrics). Binomial smoothing (18) (link) was performed and peak positions were assessed from spectrum 2 nd derivatives. Spectral deconvolution was performed as Gaussian shape by means of multipeak fitting package and the obtained peaks were used for the evaluation of relative intensity changes.
The spectra were processed by using IGOR PRO 6.05 (Wave metrics). Binomial smoothing (18) (link) was performed and peak positions were assessed from spectrum 2 nd derivatives. Spectral deconvolution was performed as Gaussian shape by means of multipeak fitting package and the obtained peaks were used for the evaluation of relative intensity changes.
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