50 μl loop

Manufactured by Waters Corporation

The 50 μL loop is a precise liquid handling device designed for use in analytical instrumentation and laboratory workflows. It provides a consistent, accurate volume of a liquid sample for introduction into an analytical system. The loop is constructed with high-quality materials to ensure reliable performance and durability.

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Lab products found in correlation

Acquity uplc h class system, by Waters Corporation (1 mentions) μdawn, by Wyatt Technology (1 mentions) Empower 3, by Waters Corporation (1 mentions) Waters alliance 1695, by Waters Corporation (1 mentions) Xselect lc 18 column, by Waters Corporation (1 mentions) Waters autosampler, by Waters Corporation (1 mentions)

2 protocols using 50 μl loop

UPLC-MALS Analysis of Biomolecules

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Experiments were performed on a Waters ACQUITY UPLC™ H-Class system equipped with a quaternary solvent manager, flow through needle autosampler with a 50 μL loop, and a tunable ultraviolet detector with a 5 mm titanium flow-cell operated at a wavelength of 280 nm (Milford, MA). The system was controlled through the Empower software.
The multi-angle light scattering (MALS) detector used consisted of a μTrex and μDawn detectors (Wyatt, Santa Barbara, CA), and were controlled with the Astra 6 acquisition and data analysis software.

Li Y., Wang X.E., Bender M.F., Yang R., Cozine T.J., Atallah K.M., Arnold F., Cooper J.W, & Lei Q.P. (2018). A Unique SEC Method Overcomes the Multiple-Monomeric-Peak Profile of the Broadly Neutralizing HIV-1 Antibody 10E8. Analytical chemistry, 90(21), 12390-12394.

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Quantification of Vitamin E in NPs

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SQAd/VitE NPs with different VitE content were formulated in 5% dextrose solution and subsequently washed with water twice using an ultrafiltration device (molecular weight cutoff, 100,000). NPs were dissolved in ethanol and measured by HPLC to determine VitE content. HPLC Waters Alliance 1695 (Waters, Milford, MA), equipped with a Waters DAD 2996 photodiode array detector, Hewlett-Packard computer with a Waters Empower 3 software, and a Waters autosampler with a 50-μl loop, was used, with simultaneous spectra detection wavelengths from 200 to 600 nm recorded for all peaks. A Waters XSelect LC-18 column (2.1 mm by 150 mm, 3.5 μm) was used with a nongradient mobile phase of acetonitrile and methanol (50:50, v/v) at a constant flow rate of 1 ml/min. The VitE peak was measured at a wavelength of 216 nm and quantitatively determined by comparing with a standard curve.

Dormont F., Brusini R., Cailleau C., Reynaud F., Peramo A., Gendron A., Mougin J., Gaudin F., Varna M, & Couvreur P. (2020). Squalene-based multidrug nanoparticles for improved mitigation of uncontrolled inflammation in rodents. Science Advances, 6(23), eaaz5466.

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