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Lamp2 alexafluor 488 h4b4

Manufactured by Thermo Fisher Scientific
Sourced in Germany

LAMP2-AlexaFluor 488 (H4B4) is a fluorescently labeled antibody that targets the LAMP2 protein. It is designed for use in immunofluorescence applications to detect and visualize the cellular localization of LAMP2.

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2 protocols using lamp2 alexafluor 488 h4b4

1

Multiplex Imaging of Cytotoxic Granule Components

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The following Abs were used against perforin (dG9), LAMP1-AlexaFluor 488 (H4A3), CD3 (UCHT1), CD56 (HCD56), NKG2D (1D11) (BioLegend); CI-MPR (2G11), dynein heavy chain, Rab27a (Abcam); EEA-1 (14/EEA1; BD Biosciences); cathepsin D, Rab7, Rab9 (Cell Signaling); LAMP1 (H4A3), LAMP2 (H4B4), granzyme B (GB7), Munc13-4, Rab14 (D-5) (Santa Cruz Biotechnology); myosin IIA, actin (AC-15) (Sigma), VAMP7 (Synaptic Systems); LAMP2-AlexaFluor 488 (H4B4) (eBiosciences); perforin (Pf-344, for immunoblotting; Mabtech).
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2

Cytotoxic Granule Trafficking Assay

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The following antibodies were used against perforin (dG9), lysosome-associated membrane protein (LAMP) 1–Alexa Fluor 488 (H4A3), CD3 (UCHT1), CD56 (HCD56), and NKG2D (1D11; BioLegend, San Diego, Calif); cation-independent mannose 6-phosphate receptor (CI-MPR; 2G11), dynein heavy chain, and Ras-associated binding protein (Rab) 27a (Abcam, Cambridge, United Kingdom); early endosome antigen 1 ([EEA-1] 14/EEA1; BD Biosciences, San Jose, Calif); cathepsin D, Rab7, and Rab9 (Cell Signaling, Danvers, Mass); LAMP1 (H4A3), LAMP2 (H4B4), granzyme B (GB7), Munc13-4, and Rab14 (D-5; Santa Cruz Biotechnology, Dallas, Tex); myosin IIA and actin (AC-15; Sigma, St Louis, Mo); vesicle associated membrane protein 7 (Synaptic Systems, Goettingen, Germany); LAMP2–Alexa Fluor 488 (H4B4) (eBioscience, San Diego, Calif); and perforin (Pf-344, for immunoblotting; Mabtech, Stockholm, Sweden).
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