Rabbit anti axl c89e7

Manufactured by Cell Signaling Technology

Rabbit anti-AXL (C89E7) is a primary antibody raised in rabbit against the AXL protein. It recognizes the AXL receptor tyrosine kinase, which is involved in cellular signaling pathways.

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Lab products found in correlation

Rabbit anti egfr, by Cell Signaling Technology (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Rat anti f4 80 sc 59171, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-AXL (11 citations) Anti-AXL (C89E7, (4 citations) Rabbit anti-AXL (3 citations) Anti-AXL C89E7 rabbit mAb (2 citations)

2 protocols using rabbit anti axl c89e7

Phosphorylated EGFR and AXL Quantification

Cited 1 time

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Western blotting was performed as already described [29 (link)]. Lysates were separated on a 3–8% SDS-PAGE. The primary antibodies used were: mouse anti-phosphorylated EGFR (Tyrosine 1068) (1H12, Cell Signaling. 1:500); rabbit anti-EGFR (#2232, Cell Signaling,1:500); rabbit anti-phosphorylated AXL (Tyrosine 703) (D12B2, Cell Signaling, 1:300); rabbit anti-AXL (C89E7, Cell Signaling, 1:1000); mouse anti-β-actina (clone C4, sc-47778. Santa Cruz Biotechnology).

Forlani L., De Cecco L., Simeon V., Paolini B., Bagnoli M., Cecere S.C., Spina A., Citeroni E., Bignotti E., Lorusso D., Arenare L., Russo D., De Angelis C., Ardighieri L., Scognamiglio G., Del Sesto M., Tognon G., Califano D., Schettino C., Chiodini P., Perrone F., Mezzanzanica D., Pignata S, & Tomassetti A. (2023). Biological and clinical impact of membrane EGFR expression in a subgroup of OC patients from the phase IV ovarian cancer MITO-16A/MANGO-OV2A trial. Journal of Experimental & Clinical Cancer Research : CR, 42, 83.

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Immunohistochemical Analysis of Liver Sections

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Paraffin molds containing liver sections were cut into 5-μm sections. The sections were deparaffinized in xylene and dehydrated in graded alcohol series. Heat-induced antigen retrieval was performed in citrate buffer. Slides were incubated with primary antibody (mouse anti-α-SMA: M0851, DAKO; rat anti-F4/80: sc-59171; Santa Cruz Biotechnology; rabbit anti-AXL: C89E7; Cell Signaling) overnight in a wet chamber at 4°C. After rinsing with PBS, the slides were incubated with fluorescent secondary antibodies for 45 minutes in a wet chamber and mounted with ProLong Gold Antifade Mountant (Invitrogen, Carlsbad, CA).

Tutusaus A., de Gregorio E., Cucarull B., Cristóbal H., Aresté C., Graupera I., Coll M., Colell A., Gausdal G., Lorens J.B., García de Frutos P., Morales A, & Marí M. (2019). A Functional Role of GAS6/TAM in Nonalcoholic Steatohepatitis Progression Implicates AXL as Therapeutic Target. Cellular and Molecular Gastroenterology and Hepatology, 9(3), 349-368.

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