Hct 116

Manufactured by ScienCell

Sourced in United States

HCT-116 is a human colorectal carcinoma cell line. It is a well-established model for studying colorectal cancer.

Automatically generated - may contain errors

Lab products found in correlation

Mcf 7, by ScienCell (3 mentions) Ccd 18, by ScienCell (2 mentions) Human umbilical vein endothelial cells (huvec), by ScienCell (1 mentions) Endothelial cell medium (ecm), by ScienCell (1 mentions) Class 2 biosafety cabinet, by Esco Lifesciences (1 mentions) Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions) Rapamycin, by MedChemExpress (1 mentions) Fk866, by Merck Group (1 mentions) Virapower hiperform t rex gateway expression system, by Thermo Fisher Scientific (1 mentions) Ht1080, by Merck Group (1 mentions) Sw1353, by Merck Group (1 mentions) Mcf 10a, by ScienCell (1 mentions) Gmx1778, by Cayman Chemical (1 mentions) Azacytidine, by Cayman Chemical (1 mentions) Decitabine, by Cayman Chemical (1 mentions) Hl 60, by ScienCell (1 mentions) Huvecs, by ScienCell (1 mentions)

5 protocols using hct 116

Culturing Human Cell Lines

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Human Umbilical Vein Endothelial Cell line HUVEC (Passage No. 3), catalogue number (C2517A); human colorectal carcinoma cell line HCT-116 (Passage No. 5), catalogue number (CCL-247); human hormone sensitive and invasive breast cancer cell line MCF-7 (Passage No. 4), catalogue number (HTB-22); human colorectal normal cell line CCD-18 (Passage No. 3), catalogue (CRL-1459) were purchased from ScienCell, USA. HUVEC were maintained in endothelial cell medium (ECM) (ScienCell, USA) supplemented with endothelial cell growth supplements (ECGS), 5% HIFBS and 1% PS. HCT-116 cells were maintained in RPMI whereas, MCF-7 and CCD-18Co were maintained in DMEM medium. The media were supplemented with 5% heat inactivated fetal bovine serum and 1% penicillin/streptomycin. Cells were cultured in a humidified incubator at 37°C supplied by 5% CO₂. Cell culture work was done in sterile conditions using Class II biosafety cabinet (ESCO, USA).

Hassan L.E., Ahamed M.B., Majid A.S., Baharetha H.M., Muslim N.S., Nassar Z.D, & Majid A.M. (2014). Correlation of antiangiogenic, antioxidant and cytotoxic activities of some Sudanese medicinal plants with phenolic and flavonoid contents. BMC Complementary and Alternative Medicine, 14, 406.

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Colon Cancer Cell Lines Treated with Rapamycin

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The normal human colon epithelial cell line NCM460 and human CRC cell lines (LoVo, RKO, HCT116, and SW480) were obtained from the Cell Band of the Chinese Academy of Science (Shanghai, China). LoVo, RKO, and SW480 cells were maintained in high glucose DMEM medium (Gibco), and HCT116 cells were cultured in McCoy's 5A medium (Sciencell). Both mediums were supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin/streptomycin in a humid incubator with 5% CO₂ at 37°C. The chemical inhibitor, rapamycin (MedChemExpress), was used to treat cells at concentrations of 50 and 100 nmol/L.

Wang T., Zhang W., Wang Z., Wu Z., Du B., Li L., Chen Y., Yang X., Hao X, & Guo T. (2019). RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1. Cancer Medicine, 9(4), 1529-1543.

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Engineered Glioblastoma TICs and Xenografts

Cited 2 times

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Glioblastoma TICs and xenografts were generated from patient tumors as previously described (Wakimoto et al., 2009 (link); Wakimoto et al., 2014 (link)). IDH1^R132H overexpressing GBM TIC (MGG18-IDH1-R132H) was generated using pLenti3.3/TR or pLenti6.3/TO/V5 containing IDH1^R132H, pCMV-dr8.2-dvpr and pCMV-VSVG (ViraPower HiPerform T-Rex Gateway Expression System, Invitrogen). The Naprt1 overexpressing IDH1 mutant GBM TIC (MGG152-Naprt1) was generated using CCSB-Broad LentiORF-NAPRT Clone (GE Dharmacon), pCMV-dr8.2-dvpr and pCMV-VSVG. Cell lines were obtained from ATCC (BT142, HT1080, U87, SW1353), Sigma-Aldrich (A431), Horizon Discovery (HCT116, MCF10A) and ScienCell (NHA). 30T and UACC257 were provided by Y.S. Chemicals were purchased from Sigma-Aldrich (FK866, GMX1778, NMN, NAD+, NA, decitabine, azacytidine, and 3-MA), or Cayman (GMX1778). For long-term IDH1i exposure, IDH1i (5 μM) or DMSO (0.1%) was added to the media 2–3 times per week.

Tateishi K., Wakimoto H., Iafrate A.J., Tanaka S., Loebel F., Lelic N., Wiederschain D., Bedel O., Deng G., Zhang B., He T., Shi X., Gerszten R.E., Zhang Y., Yeh J.R., Curry W.T., Zhao D., Sundaram S., Nigim F., Koerner M.V., Ho Q., Fisher D.E., Roider E.M., Kemeny L.V., Samuels Y., Flaherty K.T., Batchelor T.T., Chi A.S, & Cahill D.P. (2015). Extreme vulnerability of IDH1 mutant cancers to NAD+ depletion. Cancer cell, 28(6), 773-784.

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Comprehensive Cancer Cell Line Protocol

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Nine cancer cell lines and one normal cell line were used in this study: Human colorectal carcinoma cell line HCT-116; catalogue number (CCL-247); human hormone sensitive and invasive breast cancer cell line MCF-7; catalogue number (HTB-22); human colorectal normal cell line CCD-18; catalogue (CRL-1459); prostate cancer cell line PC3 catalogue number (46652); human leukemia Cell line HL-60 (CCL-240); K562 catalogue number (CCL-243); U937 catalogue number (40759), HT-29 catalogue number (HTB-38) and human hepatic carcinoma (HepG2) cells; catalogue (CRL-10741) were purchased from ScienCell, USA. HCT-116, HT-29, HL-60, K562, U937 and HepG2 cells were maintained in RPMI; MCF-7 and RGC-5 were maintained in DMEM and PC3 was maintained in F-12K medium. The media were supplemented with 5% heat inactivated fetal bovine serum and 1% penicillin/streptomycin. Cells were cultured in a humidified incubator at 37°C supplied by 5% CO₂.

Ahmed Hassan L.E., Khadeer Ahamed M.B., Abdul Majid A.S., Iqbal M.A., Al Suede F.S., Haque R.A., Ismail Z., Ein O.C, & Majid A.M. (2014). Crystal Structure Elucidation and Anticancer Studies of (-)-Pseudosemiglabrin: A Flavanone Isolated from the Aerial Parts of Tephrosia apollinea. PLoS ONE, 9(3), e90806.

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Cell Culture Protocols for Diverse Cell Lines

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Human umbilical vein endothelial cells (HUVECs), human colorectal carcinoma cell line HCT-116, human hormone sensitive and invasive breast cancer cell line MCF-7, human leukemia K562 and U937 were purchased from ScienCell, USA. HUVECs were maintained in ECM medium supplemented with 5 % HIFBS, 1 % PS and 1 % ECGS, whereas HCT-116, K562 and U937 were maintained in RPMI; MCF-7 cell line was maintained in DMEM. Cells were cultured in a humidified incubator at 37 °C supplied by 5 % CO₂.

Hassan L.E., Dahham S.S., Saghir S.A., Mohammed A.M., Eltayeb N.M., Majid A.M, & Majid A.S. (2016). Chemotherapeutic potentials of the stem bark of Balanite aegyptiaca (L.) Delile: an antiangiogenic, antitumor and antioxidant agent. BMC Complementary and Alternative Medicine, 16, 396.

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