Synthetic ssRNA (8-mer: 5’-CGACACCU-3’, 14-mer: 5’-CGACACCUGAUUCC-3’) and ssDNA (8-mer: 5’-AGGTGTCG-3’, 14-mer: 5’-GGAATCAGGTGTCG-3’) were obtained from Biologica Co. A commercial product of recombinant RNase H was from Takara Bio Inc. Ammonium acetate (NH4OAc, MS grade), trifluoroacetic acid (TFA), manganese (II) chloride tetrahydrate (99.99%), magnesium chloride hexahydrate (99.995%), zinc chloride (99.999%), calcium chloride, acetic acid, and trizma base were from Sigma-Aldrich. Sodium chloride, dithiothreitol (DTT) and hydrochloric acid were from Wako. Triethylamine (TEA), SDS, tryptone, dried extract yeast, and 0.2 mol/l-di-sodium dihydrogen ethylenediaminetetraacetate (EDTA) solution were from Nacalai Tesque. Ultrapure water was produced by PURIC-ω (ORGANO) and used for all the experiments.
Hydrochloric acid (hcl)
Manufactured by Fujifilm
Sourced in Japan
Hydrochloric acid is a clear, colorless liquid chemical compound that is widely used in various laboratory applications. It is composed of hydrogen and chlorine and has a pungent odor. Hydrochloric acid is a strong acid with a pH value typically ranging from 0 to 1, making it highly corrosive.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Fujifilm (39 mentions)
Acetic acid, by Fujifilm (15 mentions)
Methanol, by Fujifilm (15 mentions)
Ethanol, by Fujifilm (14 mentions)
Sodium chloride (nacl), by Fujifilm (14 mentions)
Trizma base, by Merck Group (6 mentions)
Sodium hydrogen carbonate, by Fujifilm (6 mentions)
Hydrogen peroxide, by Fujifilm (6 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (6 mentions)
Chloroform, by Fujifilm (5 mentions)
Toluene, by Fujifilm (5 mentions)
Acetone, by Fujifilm (5 mentions)
Ethyl acetate, by Fujifilm (5 mentions)
Ascorbic acid, by Merck Group (5 mentions)
Glucose, by Fujifilm (4 mentions)
Acetonitrile, by Fujifilm (4 mentions)
Nitric acid, by Fujifilm (4 mentions)
Ammonia solution, by Fujifilm (4 mentions)
Acetonitrile, by Kanto Chemical (4 mentions)
Triethylamine, by Fujifilm (4 mentions)
140 protocols using hydrochloric acid (hcl)
1
Enzymatic Activity of RNase H
2
Detecting Fe in OsNAS3 Knockout Leaves
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To detect the presence of Fe in the leaf tissues of OsNAS3 knockout plants, leaf sections from control and Fe excess cultures were obtained (from the second experiment) and placed in ethanol for 24 h to remove the chlorophyll. Then the sections were exposed to a solution containing 2% potassium ferricyanide (Wako Co., Ltd., Tokyo, Japan) and 2% hydrochloric acid (Wako) for 24 h. After rinsing with distilled water, the sections were mounted in distilled water and localization was observed under the Zeiss Axion Vision Image 4.2 microscope (Carl Zeiss Microscopy).
3
Synthesis and Characterization of Nanoparticles
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All reagents were used as received, without further purification. Hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl4·4H2O, 99.0%) was purchased from Kanto Chemical Co., Inc. Silver(I) nitrate (AgNO3, >99.9%) was purchased from Kojima Chemicals Co., Ltd. Tetrabutyl ammonium bromide {TBABr, [N(C4H9)4]Br, >98.0%}, triethylamine [N(C2H5)3, >99.0%], tetrakis(triphenylphosphine)palladium(0) [Pd(PPh3)4, >97.0%], and tetrakis(triphenylphosphine)platinum(0) [Pt(PPh3)4, >97.0%] were purchased from Tokyo Chemical Industry Co., Ltd. Sodium borohydride (NaBH4, 95.0%), triphenylphosphine (PPh3, 97.0%), 12 molybdo(VI) phosphoric acid n-hydrate [H3(PMo12O40)·nH2O, >95.0%], disodium molybdate(VI) dihydrate (Na2MoO4·2H2O, >99.0%), disodium tungstate(VI) dihydrate (Na2WO4·2H2O, 99.0%–100.5%), hydrochloric acid (HCl, 35.0%–37.0%), and acetic anhydride [(CH3CO)2O, >97.0%] were purchased from Wako Pure Chemical Industry.
4
Evaluating Cellular Responses to Chemical Compounds
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L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification.
5
Bamboo Cellulose-based CNF Fabrication
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Aniline, ammonium solution
(28%), ammonium persulfate (APS), m-cresol, CSA,
hydrochloric acid (HCl), and lithium chloride (LiCl) were purchased
from WAKO Chemical (Wako, Japan). CNFs in 1% water suspension, produced
by an aquatic counter-collision method from bamboo cellulose, were
provided by Chuetsu Pulp & Paper Co., Ltd. (Takaoka, Japan) and
were used as received.
(28%), ammonium persulfate (APS), m-cresol, CSA,
hydrochloric acid (HCl), and lithium chloride (LiCl) were purchased
from WAKO Chemical (Wako, Japan). CNFs in 1% water suspension, produced
by an aquatic counter-collision method from bamboo cellulose, were
provided by Chuetsu Pulp & Paper Co., Ltd. (Takaoka, Japan) and
were used as received.
6
Analytical Standards for Polycyclic Aromatic Hydrocarbons
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Benzo[b]fluoranthene (BbF, CAS 205-99-2), benzo[k]fluoranthene (BkF, CAS 207-08-9), benzo[a]pyrene (BaP, CAS 50-32-8), indeno[1,2,3-cd]pyrene (IcdP, CAS 193-39-5), nitric acid (HNO3, CAS 7697-37-2), hydrochloric acid (HCl, CAS 7647-01-0), hydrofluoric acid (HF, CAS 7664-39-3), and perchloric acid (HClO4, CAS 7601-90-3) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Fluoranthene (FR, CAS 206-44-0) and pyrene (PY, CAS 129-00-0) were obtained from Nacalai Tesque Inc. (Kyoto, Japan). Benz[a]anthracene (BaA, CAS 56-55-3), dibenz[a,h]anthracene (DahA, CAS 53-70-3), 1-nitropyrene (CAS 5522-43-0), and 2-acetylaminofluorene (CAS 53-96-3) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Chrysene (CHR, CAS 218-01-9), benzo[ghi]perylene (BghiP, CAS 191-24-2), phenobarbital (CAS 50-06-6), and β-naphthoflavone (CAS 6051-87-2) were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Quartz filters were obtained from Pall Life Sciences (Port Washington, NY, USA).
7
Ammonium Persulfate Functionalization Protocol
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Ammonium persulfate (APS), hydrochloric acid (HCl), aminophenyl boronic acid (APBA), aniline (ANI), polyvinyl alcohol (PVA), and glucose were purchased from Wako Pure Chemical Industries, Ltd. Phosphate-buffered saline (1× PBS, pH 7.4) was purchased from Thermo Fisher Scientific Inc.
8
Synthesis and Characterization of Gold Nanoparticles
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Hydrogen tetrachloroaurate hydrate (HAuCl4.xH2O, 99.9%), sodium borohydride (NaBH4 99%), silver nitrate (AgNO3, 99%), hexadecyltrimethylammonium bromide (CTAB, ≥99%), ascorbic acid (99%), sodium oleate (NaOL, ≥99%), Methoxypolyethylene glycol thiol (mPEG-SH) (MW 5000 Da), Dulbecco’s Modified Eagle’s Medium (DMEM), phosphate saline buffer (PBS, Ca2+ and Mg2+ free, pH = 7.4), and 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich, South Africa and Japan. Hydrochloric acid (HCl, ACS reagent 37 wt.%) was obtained from Wako. Gelatin from porcine skin was obtained from Fluka, Japan. All chemicals were of analytical grade and were used without further purification. All glassware used in the experiment was cleaned and washed thoroughly with MilliQ water (18.2 MΩ cm @ 25 °C) and dried before use.
9
Amino Acid Profiling in Vegetables and Pulses
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A Hitachi L-8900 Automatic Amino Acid Analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan) with a 4.6 mm (ID)×60 mm ion exchange column (Hitachi High-Technologies Corporation) was used to determine the amino acid profiles of the vegetable and pulse samples. The following analyzer settings were used for the analysis: buffer flow rate of 0.4 mL/min, reagent flow rate of 0.35 mL/min, reactor heater temperature of 135°C, column temperature of 75°C, auto-sampler temperature of 5~8°C, run time of 35.3 min (sulfur-containing amino acids) or 56.3 min (all other amino acids), sample injection volume of 20 μL, and detection wavelength of 570 nm (proline) or 440 nm (all other amino acids). Protein hydrolysate buffer set (PH-SET KANTO, Kanto Chemical Co., Inc., Tokyo, Japan) and hydrochloric acid (Wako Pure Chemical Industries, Ltd.) were used as mobilephase solvents (10 (link),11 ). A standard amino acid mixture of cysteic acid and methionine sulfone (20 μL/mL) was diluted to 100 μmol/L for amino acid quantification and calibration. An external standard was used to calculate the concentration of each amino acid. Amino acid concentrations are reported in g/100 g.
10
Determination of Aluminum by ICP-AES
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Hydrochloric acid (HCl) (35.0–37.0%) for measurement of toxic metals, nitric acid (HNO3) (60–62%) for precision analysis, 0.1 mol/L HNO3 for quantitative analysis, Al standard solution (100 μg/mL, certified by Japan Calibration Service System), and Ultrapure Water (H2O) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Calibration standard solutions of Al (0.012, 0.06, 0.3, 1.5, and 7.5 μg/mL) were prepared by dilution of Al standard solution with 0.1 mol/L HNO3.
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