Rhodamine b

Manufactured by Maclin Biochemical Technology

Sourced in China, United States

Rhodamine B is a fluorescent dye commonly used in various scientific and industrial applications. It has an excitation wavelength of approximately 540 nanometers and an emission wavelength of around 625 nanometers. Rhodamine B is a versatile tool for labeling, staining, and detection purposes in fields such as microscopy, flow cytometry, and fluorescence-based assays.

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Lab products found in correlation

Methylene blue, by Maclin Biochemical Technology (4 mentions) Diethyl ether, by Guangzhou Chemical (3 mentions) Acetone, by Guangzhou Chemical (3 mentions) Hydrochloric acid (hcl), by Guangzhou Chemical (3 mentions) Chitosan, by Maclin Biochemical Technology (2 mentions) Oxymatrine, by Maclin Biochemical Technology (2 mentions) Methyl orange, by Maclin Biochemical Technology (2 mentions) Sodium lignosulfonate, by Maclin Biochemical Technology (1 mentions) Congored, by Maclin Biochemical Technology (1 mentions) Potassium dichromate k2cr2o7, by Sinopharm (1 mentions) Fluorescein isothiocyanate (fitc), by Beyotime (1 mentions) Fitc labeled goat anti rabbit igg h l, by Beyotime (1 mentions) 1 octadecene, by Maclin Biochemical Technology (1 mentions) Annexin 5 fitc pi apoptosis assay kit, by Beyotime (1 mentions) Dapi staining kit, by Beyotime (1 mentions) Tunel apoptosis assay kit, by Beyotime (1 mentions) Methanol, by Sinopharm (1 mentions) He staining kit, by Beyotime (1 mentions) Atp assay kit, by Beyotime (1 mentions) Hematoxylin and eosin, by Beyotime (1 mentions)

Close spelling variants of this product

Rhodamine B (RhB), (4 citations) Rhodamine B isothiocyante (RBITC) (2 citations)

20 protocols using rhodamine b

Cationic Polymer-Based Dye Adsorption

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Sodium lignosulfonate (C₂₀H₂₄Na₂O₁₀S₂,
MW 534.51 g/mol, 96%), chitosan (C_6nH_11nNO_4n, 95% deacetylated,
viscosity 100–200 mPa s), Rhodamine B (C₂₈H₃₁ClN₂O₃, MW 479.01 g/mol, AR), and Congo
red (C₃₂H₂₂N₆Na₂O₆S₂, MW 696.66 g/mol, BS) were obtained from Shanghai
Macklin Biochemical Co., Ltd. Potassium dichromate (K₂Cr₂O₇, MW 294.18 g/mol, GR) was provided by Sinopharm
Chemical Reagent Co., Ltd. Acetic acid (CH₃COOH, MW 60.05
g/mol, AR) was purchased from Beijing Chemical Works. In addition,
all other solutions were prepared by distilled water. All chemicals
in the experiments were used without further purification.

Gu F., Geng J., Li M., Chang J, & Cui Y. (2019). Synthesis of Chitosan–Ignosulfonate Composite as an Adsorbent for Dyes and Metal Ions Removal from Wastewater. ACS Omega, 4(25), 21421-21430.

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Functionalized Silica-Protein Nanoconjugates

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All chemicals were available commercially and the solvents were purified by conventional methods before use. Rhodamine B was purchased from Shanghai Macklin Biochemical Co., Ltd. Tetraethyl orthosilicate (TEOS), (3-Aminopropy) triethoxysilane (APTES) and 3-(Triethoxysilyl) propylisocyanate were purchased from Aladdin Industrial. Bovine Hemoglobins (BHb), Lecithos (Lec), Lysozyme (Lys), Pepsin (Pep), Horseradish Peroxidase (HRP), Casein (Cas), Transferrin (TRA), Ovalbumin (Alb) were purchased from Shanghai Lanji biological technology Co., Ltd. 3-Isocyanatopropyltriethoxysilane was purchased from Nanjing Jingruijiu biological technology Co., Ltd.

Zhang W., Wang J., Li P., Wu C., Zhang H., Zhang W., Wang H, & Tang B. (2017). Transferrin-navigation Nano Artificial Antibody Fluorescence Recognition of Circulating Tumor Cells. Scientific Reports, 7, 10142.

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Optimizing α-MnO2 Catalysis for Rhodamine B Degradation

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All chemicals were of analytical grade and used directly without further purification, unless otherwise specified. α-MnO₂ were fabricated using KMnO₄ and MnSO₄·H₂O as precursors. Peroxymonosulfate (2KHSO₅·KHSO₄·K₂SO₄) and Rhodamine B (RhB) were bought from Macklin Biochemical Technology and used as received. Potassium chloride (KCI), sodium bicarbonate (NaHCO₃), and monopotassium phosphate (KH₂PO₄) were used to investigate the cation influence of the RhB degradation efficiency. The radical scavengers used in this study included tert-Butanol (TBA), p-Benzoquinone (p-BQ), methanol (MeOH), and furfuryl alcohol (FFA), respectively. The pH value was adjusted using sulfur acid solution and sodium hydroxide.

Li J., Shi Q., Sun M., Liu J., Zhao R., Chen J., Wang X., Liu Y., Gong W., Liu P, & Chen K. (2023). Peroxymonosulfate Activation by Facile Fabrication of α-MnO2 for Rhodamine B Degradation: Reaction Kinetics and Mechanism. Molecules, 28(11), 4388.

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Oxymatrine-Loaded Liposomes for Targeted Drug Delivery

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Oxymatrine (OMT, purity ≥ 98%, lot no. C10572744), rhodamine B (RB, purity ≥ 99%, lot no. C10006604), and chitosan (CS, 800~1000 Da, lot no. C13207154) were offered by Macklin Biochemical Co., Ltd. (Shanghai, China). Cholesterol (Chol, purity ≥ 92.5%, lot no. WXBB1238) was purchased from VetecTM reagent grade (Shanghai, China). 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, lot no. CC0657) was provided by Corden pharma Switzerland LLC (Liestal, Switzerland). Hydrogenated soybean phosphatidylcholine (HSPC, lot no. B60455) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG, lot no. B90497) were supported by A.V.T Pharmaceutical Co., Ltd. (Shanghai, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy (polyethylene glycol) (DSPE-PEG_2K, lot no. RS0200412) was purchased from Xi’an ruixi Biological Technology Co., Ltd. (Xi’an, China).

Peng J., Wang Q., Guo M., Liu C., Chen X., Tao L., Zhang K, & Shen X. (2022). Development of Inhalable Chitosan-Coated Oxymatrine Liposomes to Alleviate RSV-Infected Mice. International Journal of Molecular Sciences, 23(24), 15909.

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Silk Fibroin Nanospheres with Encapsulated Bioactives

Cited 1 time

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SF nanospheres were prepared through phase separation between SF and PVA (Macklin, Shanghai, China) [24 (link),27 (link)]. In brief, SF solution and PVA solution with the same initial concentration (0.2%, 1% and 5%) was mixed with the volume ratio of 1/4. To fabricate the drug-loaded nanospheres, 100 μg/ml E7 peptide (EPLQLKM, Scilight-Peptide, Beijing, China), 1 μg/ml FITC-labeled E7 (Scilight-Peptide, Beijing, China), 10 μM KGN (Sigma-Aldrich, St Louis, MO, USA) or 1 μg/ml Rhodamine B (RhoB, Macklin, Shanghai, China) were added to the solution. The blend solution was sonicated with an X0-400S ultrasonic processer (Atpio Co., Nanjing, China) at 25% amplitude for 30 s. The solution was poured into a petri dish to evenly cover the bottom of the dish, and was dried overnight in fume hood. The dried film was dissolved in ultrapure water under shaking for 30 min and centrifuged at 11000 r.p.m. at 4 °C for 20 min, to remove the residual PVA from the SF spheres. The supernatant was collected for the measurement of drug loading efficiency. After removing the supernatant, the pellet was suspended in ultrapure water to obtain the SF nanosphere suspension.

Zhang W., Ling C., Zhang A., Liu H., Jiang Y., Li X., Sheng R., Yao Q, & Chen J. (2020). An all-silk-derived functional nanosphere matrix for sequential biomolecule delivery and in situ osteochondral regeneration. Bioactive Materials, 5(4), 832-843.

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Cytotoxicity and Apoptosis Evaluation

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LuCl₃, cesium acetate (CsAc), lactic acid (LA), oleic acid (OA), 1-octadecene (ODE) and Rhodamine B (RhB) were purchased from Macklin. NH₄F, methanol, ethanol and cyclohexane were purchased from Sinopharm Chemical Reagent Co., Ltd. Phosphate buffered solution (PBS), dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Adamas Life. Cell counting kit-8 (CCK-8), histone H2AX rabbit polyclonal antibody, fluorescein isothiocyanate (FITC), FITC-labeled goat anti-rabbit IgG (H + L), DAPI staining kit, Ki67 staining kit, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) assay kit, 2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay kit, ATP assay kit, hematoxylin and eosin (H&E) staining kit, TUNEL apoptosis assay kit, calcein/PI cell viability/cytotoxicity assay kit, Annexin V-FITC/PI apoptosis assay kit were bought from Beyotime.

Liu H., Wang H., Ni D, & Xu Y. (2022). Lactic acid modified rare earth-based nanomaterials for enhanced radiation therapy by disturbing the glycolysis. Journal of Nanobiotechnology, 20, 490.

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Polymer-based Photocrosslinkable Hydrogel Synthesis

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Acrylamide (Am, 99.9%), acryloyl chloride (96%), choline chloride (ChCl, 99%), β-cyclodextrin (β-CD, 98%), dichloromethane (DCM, 99.9%, with molecular sieves, water ≤ 50 ppm), 4-dimethylaminopyridine (DMAP, 99%), N,N-dimethylformamide (DMF, 99.8%, with molecular sieves, water ≤ 50 ppm), 4-(4,6-dimethoxy−1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), ethylene glycol (EG, 98%), poly(ethylene glycol) (PEG, average Mn = 2000), rhodamine B, and tartrazine (yellow dye) were purchased from Macklin. 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I₂₉₅₉, ≥98.0%), lithium phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP, ≥98.0%), lithocholic acid (LCA, >97.0%), N,N’-methylenebisAcrylamide (MBA), sodium bicarbonate (NaHCO₃, ≥99.8%), and triethylamine (TEA, ≥99.5%) were purchased from Aladdin. Acetone, diethyl ether and hydrochloric acid (HCl) were purchased from Guangzhou Chemical Reagent Factory (Guangdong, China).

Xiong X., Chen Y., Wang Z., Liu H., Le M., Lin C., Wu G., Wang L., Shi X., Jia Y.G, & Zhao Y. (2023). Polymerizable rotaxane hydrogels for three-dimensional printing fabrication of wearable sensors. Nature Communications, 14, 1331.

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Synthesis and Characterization of Polymeric Membranes

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PAN polymer was purchased from Dechao Chemical Co. Ltd. (Hubei, China). Sodium sulfate (Na₂SO₄), sodium hydroxide (NaOH), magnesium chloride (MgCl₂), calcium chloride (CaCl₂), sodium chloride (NaCl), sodium hydrogen phosphate (Na₂HPO₄), nickel sulfate (NiSO₄), magnesium sulfate (MgSO₄), N,N-dimethylformamide (DMF), and n-hexane were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Methyl orange, CR, AB93, methylene blue, crystal violet, and rhodamine B were provided by Macklin Biochemical Co. Ltd. (Shanghai, China). TC, CLP, and BAC were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). TMC, BSA, and HA were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). St was purchased from Nuoyi Biotechnology Co. Ltd. (Zhejiang, China). Polyethylene glycols (PEGs; 200, 400, 600, 1000, 1500, 2000 and 4000 Da) were obtained from Macklin Biochemical Co. Ltd. (Shanghai, China) and used to evaluate MWCO of membranes. The water used in all experiments was deionized water. All chemicals were used as received without further purification.

Bai Y., Liu B., Li J., Li M., Yao Z., Dong L., Rao D., Zhang P., Cao X., Villalobos L.F., Zhang C., An Q.F, & Elimelech M. (2023). Microstructure optimization of bioderived polyester nanofilms for antibiotic desalination via nanofiltration. Science Advances, 9(18), eadg6134.

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Soybean Protein Isolate Characterization

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Soybean protein isolate (dispersed, protein > 90%) and the glucono-δ-lactone (BR grade) were provided by Shanghai Macklin Biochemical Co., Shanghai, China; soybean oil (food grade) was purchased from Shenzhen Nanrui Grain and Oil Foodstuff Co., Shenzhen, China; Rhodamine B was purchased from Shanghai Macklin Biochemical Co., Shanghai, China.

Bi C., Zhou T., Wu Z, & Huang Z. (2023). Acid-Mediated Formation of Soybean Isolate Protein Emulsion Gels with Soybean Oil as an Active Component. Foods, 12(9), 1754.

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Targeted Delivery of Oxymatrine via SPEG-CMCS Nanoparticles

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Oxymatrine (OMT), polyoxyethylene stearate (SPEG), rhodamine B (RB), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI) and carboxymethyl chitosan (CMCS, N-linked, carboxylation degree 80%) were offered by Macklin Biochemical Co., Ltd. (Shanghai, China), respectively. Octadecylamine (ODA) and N-Hydroxysuccinimide (NHS) were supplied by Adamas-beta (Shanghai, China). Soybean phospholipid (SPC) was supplied by A.V.T Pharmaceutical Co., Ltd. (Shanghai, China). Cholesterol (Chol) was purchased from VetecTM reagent grade (Shanghai, China). Porcine pancreatic elastase (PPE) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China).

Peng J., Cai Z., Wang Q., Zhou J., Xu J., Pan D., Chen T., Zhang G., Tao L., Chen Y, & Shen X. (2022). Carboxymethyl Chitosan Modified Oxymatrine Liposomes for the Alleviation of Emphysema in Mice via Pulmonary Administration. Molecules, 27(11), 3610.

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