Euroflush

Manufactured by IMV Technologies

Sourced in France

Euroflush is a laboratory equipment product designed for flushing and cleaning. It provides a reliable and efficient way to maintain and clean various laboratory instruments and equipment.

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Lab products found in correlation

Stereo zoom microscope, by Olympus (2 mentions)

7 protocols using euroflush

Antibiotic-free Embryo Collection Protocol

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80 prim'holstein donors (12.9 months average age)donors were divided into two groups, a control group, batch A: n=40; collected with Euroflush ND with antibiotics (ND IMV technologies, 61300 L'Aigle France with antibiotics) and a second group, batch B: n=40; collected with Euroflush without antibiotics, IMV technologies, 61300 L'Aigle France) without antibiotics. The rest of the procedure was identical in both batches, the embryos underwent ten washes containing gentamicin 0.05g/L and kanamycin 0.1g/L. 80 Prim'holstein donors (12.9 months average age) were divided into two groups, " Batch A (control group) : n=40; collected with Euroflush ND with antibiotics (IMV technologies) " Batch B: n=40; collected with Euroflush without antibiotics (IMV technologies).
All the embryos underwent ten washes containing gentamicin 0.05g/L and kanamycin 0.1g/L before transfer (fresh or frozen).
The sample from the last wash was tested for bacterial count (<100 CFU/mL considered free from pathogens) , the sample from the collection medium was tested for mycoplasma .
The size of this study was determined considering a logistic regression model, with a probability of non-infection of 0.99 for the standard procedure (with antibiotics) and of 0.95 for the batch without antibiotics, a power of 0.8 and a significance level of 0.05.

Marquet M., Ben Hania W., Thorin C., Guilbert-Julien L., Quinton H., Pol J., Escouflaire P., Chevrier L., Gard J., Chavatte-Palmer P, & Briand-Amirat L. (2021). 82 Are antibiotics still truly needed in bovine embryo collection media? A preliminary study. Reproduction, fertility, and development, 34(2).

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Ovarian Stimulation and Oocyte Collection in Cattle

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For Holstein heifers, ovarian stimulation was performed as previously described [3 (link)] 36 h after the removal of the dominant follicles. Briefly, a new intravaginal progesterone device (Prid^® Delta, 1.55 g, Ceva, Libourne, France) was inserted, and the ovarian stimulation was performed by five intramuscular injections of decreasing porcine follicle stimulating hormone/porcine luteinizing hormone (pFSH/pLH) doses (Stimulfol^®, Reprobiol, Ouffet, Belgium), every 12 h, over 2.5 days.
For Holstein lactating cows, ovarian stimulation was performed as described by Freret et al. [14 ] by five intramuscular injections of decreasing pFSH/pLH doses (Stimulfol^®, Reprobiol, Ouffet, Belgium), every 12 h, over 2.5 days.
Cumulus oocyte complexes (COCs) were collected by OPU. After locoregional anesthesia, follicles from both ovaries, from 6 to 12 mm in diameter, were aspirated by transvaginal way using ultrasonography and COCs were recovered in a tube containing 1 mL of flushing solution (Euroflush^®; IMV Technologies, L’Aigle, France) added with heparin (1:50), maintained at 37°C.

Janati Idrissi S., Le Bourhis D., Lefevre A., Emond P., Le Berre L., Desnoës O., Joly T., Buff S., Freret S., Schibler L., Salvetti P, & Elis S. (2021). Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile. Biology of Reproduction, 106(3), 597-612.

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In vivo Bovine Embryo Production

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For in vivo embryo production, cows were bred through artificial insemination (AI) following induced estrus. Synchronization was carried out via a superovulation treatment (eight degressive doses of Stimufol^®, Reprobiol, Soiron-Pepinster, Belgium; one Stimufol dose contained 500 mg porcine FSH and 100 mg porcine LH), as detailed in the work of Richard et al. (2015) (link).
AI was conducted using one batch of frozen sperm from a single Holstein bull; the same batch was used for the in vitro-produced embryos. Bovine embryos were collected on day 12 and day 15 after insemination via non-surgical recovery by flushing the uterine horns with PBS (Richard et al., 2015 (link)). The flushing solution (Euroflush, IMV Technologies, France) was heated to 38°C at the beginning of the experiment and maintained at 30°C in a thermostatic box throughout the collection period.

Degrelle S.A., Liu F., Laloe D., Richard C., Le Bourhis D., Rossignol M.N, & Hue I. (2024). Understanding bovine embryo elongation: a transcriptomic study of trophoblastic vesicles. Frontiers in Physiology, 15, 1331098.

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In Vitro Maturation of Bovine Oocytes

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The recovered COCs were selected under a stereomicroscope. Only COCs with homogenous, non-granular cytoplasm and having at least 3 layers of granulosa cells were used^{56 (link)}. The COCs were washed at 37 °C, three times in embryo flushing media (Euroflush, IMV Technologies, France). The fourth time, COCs were washed in in vitro maturation medium, which consisted in TCM-199, supplemented with 10% FCS (v/v), 10 μg/mL pFSH/pLH, 1 μg/mL 17β-œstradiol, 5 ng/mL epidermal growth factor and 5 μg/mL gentamicin. All COCs were incubated at 38.5 °C for 22 h under a maximum humidity atmosphere of 5% CO₂ in the air.

Janati Idrissi S., Le Bourhis D., Lefevre A., Emond P., Le Berre L., Desnoës O., Joly T., Buff S., Maillard V., Schibler L., Salvetti P, & Elis S. (2021). Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process. Scientific Reports, 11, 11618.

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Equine Oocyte Harvest and Transport

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Oocyte collection Immature oocytes were collected by transvaginal aspiration (TVA) of antral follicles from 163 donor mares at any stage of the oestrous cycle, as described by Claes et al. (2019) (link). Mares were premedicated with detomidine hydrochloride (0.01 mg kg À1 , i.v.), butorphanol tartrate (0.01 mg kg À1 ), flunixin meglumine (1.1 mg kg À1 , i.v.) and broad-spectrum antibiotics (6.6 mg kg À1 , i.v., gentamycin sulfate, 20 000 IU kg À1 , i.m., benzylpenicillin); epidural anaesthesia (8 mL of 2% lignocaine) was induced before TVA. Antral follicles .5 mm were punctured using a 12-gauge double-lumen needle and flushed 8-10 times with medium (Euroflush, IMV Technologies) supplemented with heparin sodium (20 IU mL À1 ) to prevent blood clotting. Recovered follicular and flushing fluids were filtered (70-mm filter) and the fluid residue in the filter was poured into a sterile Petri dish. Oocytes were retrieved using a dissecting microscope, washed three times with modified HEPES-buffered synthetic oviductal fluid (mH-SOF), placed into mH-SOF and shipped overnight at 228C in prewarmed polystyrene organ transplant boxes to the assisted reproductive technology laboratory (Avantea, Italy) for the in vitro production of blastocysts.

Claes A., Cuervo-Arango J., Colleoni S., Lazzari G., Galli C, & Stout T.A. (2020). Speed of in vitro embryo development affects the likelihood of foaling and the foal sex ratio. Reproduction, fertility, and development, 32(5).

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Superovulation and Embryo Collection in Ewes

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Embryos were produced from superovulated donor ewes using purified porcine FSH given in six tapering dose protocol and eCG, injection given at the start of first FSH given on day 10 after application of progesterone sponge. Donor ewes were treated in two FSH dose groups with/without hCG hormone given 24h after removal of implant, thus making four groups Group-I (80 mg FSH), Group-II (80 mg FSH+ 500 mg hCG), Group-III (100 mg FSH), Group-IV (100 mg FSH+ 500 mg hCG). On observation of estrus donor ewes were hand mated once and further same breeding ram was allowed to accompany over night with them.
Embryo collection was performed surgically after 5-6 days of breeding date in donor ewes.
Retrograde flushing of the uterine horns were done using flushing media (Euroflush, IMV Technologies) with the help of IV cannulla introduced near the bifurcation of uterine horns. The washings were collected through catheterization of fallopian tubes and collected in sterile beaker. Searching, isolation, evaluation and gradation of embryos were performed under stereo zoom microscope (Olympus, Japan) at 200X and were classified morphologically as per Gibbons and Cueto (2011) .

Pandey A., Sharma U., Kumar S., Kumar S., Pande N., Kaul A., Andrabi S.E.H., & Saikia B. (2020). Laparoscope Assisted Embryo Transfer and Conception Rate in Farm Rambouillet Ewes. International Journal of Current Microbiology and Applied Sciences, 9(6), 3605-3612.

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Embryo Retrieval via Retrograde Uterine Flushing

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Retrograde flushing of the uterine horns was performed by catheterization of fimbriated end of the fallopian tube using 5G infant feeding tube and introduction of flushing media (Euroflush, IMV Technologies) using IV cannula inserted near the bifurcation of uterine horns(D' Alessandro et al., 2001). The washings collected into sterile petri dishes were used for searching, evaluation and gradation of embryos under stereo zoom microscope (Olympus, Japan) at 200X using IETS recommendations. The data was analysed as per Snedecor and Cochran (1994) [31] .

Pandey A., Sharma U., Kaul A., Kumar S., Pande N., & Andrabi S.E.H. (2020). Response to FSH (Folltropin-V) and hCG treatment on superovulation and embryo production in sheep under sub-tropical climate. The Pharma Innovation, 9(6), 375-380.

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