Au1000

Manufactured by Olympus

Sourced in Japan

The AU1000 is a clinical chemistry analyzer designed for use in medical laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests, providing accurate and reliable results. The AU1000 utilizes proven analytical technologies to deliver efficient and high-throughput testing.

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Lab products found in correlation

Elisa kit, by R&D Systems (3 mentions) Elisa, by R&D Systems (1 mentions) Ie33 system, by Philips (1 mentions)

32 protocols using au1000

Quantifying Liver Enzyme Levels

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Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the collected serum were analyzed using an automated clinical analyzer (OLYMPUS AU1000; Olympus, Tokyo, Japan).

Wang C., Chen K., Xia Y., Dai W., Wang F., Shen M., Cheng P., Wang J., Lu J., Zhang Y., Yang J., Zhu R., Zhang H., Li J., Zheng Y., Zhou Y, & Guo C. (2014). N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway. PLoS ONE, 9(9), e108855.

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Serum Enzyme Measurement Protocol

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After blood collection, serum was separated by centrifugation at 2,000 rpm for 10 minutes at room temperature. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were tested using an automated chemistry analyzer (Olympus AU1000; Olympus Corporation, Tokyo, Japan).

Cheng P., Chen K., Xia Y., Dai W., Wang F., Shen M., Wang C., Yang J., Zhu R., Zhang H., Li J., Zheng Y., Wang J., Zhang Y., Lu J., Zhou Y, & Guo C. (2014). Hydrogen sulfide, a potential novel drug, attenuates concanavalin A-induced hepatitis. Drug Design, Development and Therapy, 8, 1277-1286.

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Serum Biomarker Analysis Protocol

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After taken from their hearts, blood samples were placed at 4°C for 4-5 hours, and then, serum was separated by centrifuging at 4600 ×g for 10 minutes, finally storing at −80°C. The levels of serum ALT and AST were measured using an automated chemical analyzer (Olympus AU1000, Olympus, Tokyo, Japan). The plasma levels of TNF-α and IL-6 were measured using ELISA kits following the appropriate protocols.

Wu L., Zhang Q., Dai W., Li S., Feng J., Li J., Liu T., Xu S., Wang W., Lu X., Yu Q., Chen K., Xia Y., Lu J., Zhou Y., Fan X, & Guo C. (2017). Quercetin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy by Inhibiting ERK/NF-κB Pathway. Gastroenterology Research and Practice, 2017, 9724217.

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Liver Enzyme Assays and Hydroxyproline

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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using an automated chemistry analyzer (Olympus AU1000; Olympus Corporation, Tokyo, Japan). Hydroxyproline and LN were determined using kits according to the manufacturers’ instructions.

Li J., Chen K., Li S., Feng J., Liu T., Wang F., Zhang R., Xu S., Zhou Y., Zhou S., Xia Y., Lu J., Zhou Y, & Guo C. (2016). Protective effect of fucoidan from Fucus vesiculosus on liver fibrosis via the TGF-β1/Smad pathway-mediated inhibition of extracellular matrix and autophagy. Drug Design, Development and Therapy, 10, 619-630.

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Liver Function and Injury after I/R

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To detect liver function and cellular injury following liver I/R injury, sALT and sAST levels were measured using the automated clinical analyzer (OLYMPUS AU1000; Olympus, Tokyo, Japan).

Tong Y., Ding X.B., Chen Z.X., Jin S.Q., Zhao X., Wang X., Mei S.Y., Jiang X., Wang L, & Li Q. (2016). WISP1 mediates hepatic warm ischemia reperfusion injury via TLR4 signaling in mice. Scientific Reports, 6, 20141.

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Serum Separation and Enzyme Measurement

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After blood collection, serum was separated by centrifugation at 4,300× g for 10 minutes at room temperature. Serum AST and ALT were measured by an automated chemistry analyzer (Olympus AU1000, Olympus, Tokyo, Japan).

Li S., Xia Y., Chen K., Li J., Liu T., Wang F., Lu J., Zhou Y, & Guo C. (2016). Epigallocatechin-3-gallate attenuates apoptosis and autophagy in concanavalin A-induced hepatitis by inhibiting BNIP3. Drug Design, Development and Therapy, 10, 631-647.

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Serum Biomarkers of Liver IRI

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Blood samples were collected by removing the eyes. Serum was separated from the blood by centrifugation at 2,000× g at 4°C for 10 min and stored at −80°C. Serum levels of ALT and AST were measured in an automated chemical analyzer (Olympus AU1000, Olympus, Tokyo, Japan) to evaluate liver IRI. Serum levels of IL-6 and TNF-α were measured using ELISA kits according to the manufacturers’ protocols.

Feng J., Zhang Q., Mo W., Wu L., Li S., Li J., Liu T., Xu S., Fan X, & Guo C. (2017). Salidroside pretreatment attenuates apoptosis and autophagy during hepatic ischemia–reperfusion injury by inhibiting the mitogen-activated protein kinase pathway in mice. Drug Design, Development and Therapy, 11, 1989-2006.

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Liver Injury and Oxidative Stress Assessment

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Blood was obtained via cardiac puncture, and serum was isolated. To assess liver function and cellular injury following liver I/R, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured using the automated chemistry analyzer (OLYMPUS AU1000; Olympus, Tokyo, Japan). Liver tissues (cephalad lobes) were fixed in neutral buffered formalin, embedded in paraffin, and sections (5 μm) stained with hematoxylin and eosin (H&E) for histological analysis. The severity of liver I/R injury was graded blindly using Suzuki’s criteria on a scale from 0 to 4. Samples with no necrosis, congestion, or centrilobular ballooning were given a score of 0 while severe congestion and > 60% lobular necrosis were assigned a value of 4. In addition, endothelial dysfunction was scored as follows: 0, absent; 1, mild endothelial dysfunction; 2, severe endothelial dysfunction; 3, 2 + infiltration of regenerating endothelial cells and inflammatory cells; and 4, 3 + vascular stenosis. For assessing the oxidation–reduction status of liver, the malondialdehyde (MDA) level was measured via the thiobarbituric acid method using liver homogenates. MDA detection was conducted according to the manufacturer’s instructions with the aid of commercial kits (Nanjing Jiancheng Biological Institute, Jiangsu, China) and concentrations expressed as nanomoles per milligram of protein.

He Z., Li Y., Ma S., Yang M., Ma Y., Ma C., Song J., Yu T., Zhang S, & Li J. (2018). Degranulation of gastrointestinal mast cells contributes to hepatic ischemia–reperfusion injury in mice. Clinical Science (London, England : 1979), 132(20), 2241-2259.

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Serum Enzyme Quantification Protocol

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After blood collection, serum was separated by centrifugation at 3000 rpm for 15 min at room temperature. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested using an automated chemistry analyzer (Olympus AU 1000; Olympus Corporation, Tokyo, Japan).

Zhou Y., Chen K., He L., Xia Y., Dai W., Wang F., Li J., Li S., Liu T., Zheng Y., Wang J., Lu W., Yin Q., Zhou Y., Lu J., Teng H, & Guo C. (2015). The Protective Effect of Resveratrol on Concanavalin-A-Induced Acute Hepatic Injury in Mice. Gastroenterology Research and Practice, 2015, 506390.

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Liver Function Biomarkers Evaluation

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Serum levels of alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TBIL), and total cholesterol (TCHO) were determined by an automated biochemical analyzer (Olympus AU1000; Olympus Corporation, Tokyo, Japan). Liver triglyceride (TG) levels were measured with a TG quantification kit according to a protocol provided by the manufacturer (cat. no. E1013, Applygen Technologies, Beijing, China). Total hepatic hydroxyproline content was measured with a commercial detection kit following the manufacturer's instructions (cat. no. A030, Jiancheng Biotechnology Company, Nanjing, China). 11 Fasting serum insulin (FINS) was measured using an Ultrasensitive Mouse Insulin ELISA kit (cat. no. EZRMI-13K, Millipore, Billerica, MA, USA). Homeostasis model assessment-insulin resistance (HOMA-IR) index was calculated based on the following formula: 12 HOMA-IR = (FINS (mU/l) × fasting serum glucose (mmol/l))/22.5.

Xu G., Ye J., Liu X.J., Zhang N.P., Zhao Y.M., Fan J., Liu X.P., Wu J, & Wu J. (2017). Activation of pluripotent genes in hepatic progenitor cells in the transition of nonalcoholic steatohepatitis to pre-malignant lesions. Laboratory investigation; a journal of technical methods and pathology, 97(10).

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