rz2 bioamp processor, by Tucker-Davis Technologies - Product details

1

Robotic Ankle Stimulation and EMG Recording

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Lewis rats (n=4) were lightly anesthetized (Ketamine: 75 mg/kg
and Xylazine 5 mg/kg, ip) and positioned in a prone position
within a support system that let the hindlimbs hanging freely. The right paw
was secured within a 3D printed pedal connected to a stepper motor
(QSH4218-51-10-049, Trinamic Motion Control GmbH, Waterloohain, Germany). We
used this robotic platform to impose cyclic movements of the ankle with a
fixed amplitude (70 degrees) and frequency (0.54 Hz), while continuous EES
was delivered to evoke responses in the tibialis anterior muscle (Figure 3e). EES was delivered using an
IZ2H Stimulator controlled by a RZ2 BioAmp Processor (Tucker-Davis
Technologies, Alachua, US). EES amplitude was set to approximately 1.2 times
the muscle response threshold. We tested EES frequencies ranging from 5 to
100 Hz, delivered in a random order. EMG activity of the tibialis anterior
was amplified with a PZ3 Low Impedance Amplifier (Tucker-Davis Technologies,
Alachua, US) and recorded with the RZ2 BioAmp Processor at a sampling
frequency of 24414 Hz. Ankle kinematics was record with the
Vicon motion capture system at sampling frequency of 200 Hz. For each tested
EES condition a minimum of 1 minute of recording was performed. To analyze
the modulation of the muscle responses, we used the same procedures that we
adopted in the equivalent experiment carried out in human subjects.

Formento E., Minassian K., Wagner F., Mignardot J., Le Goff C.G., Rowald A., Bloch J., Micera S., Capogrosso M, & Courtine G. (2018). Electrical spinal cord stimulation must preserve proprioception to enable locomotion in humans with spinal cord injury. Nature neuroscience, 21(12), 1728-1741.

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Robotic Ankle Stimulation and EMG Recording

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Lewis rats (n=4) were lightly anesthetized (Ketamine: 75 mg/kg
and Xylazine 5 mg/kg, ip) and positioned in a prone position
within a support system that let the hindlimbs hanging freely. The right paw
was secured within a 3D printed pedal connected to a stepper motor
(QSH4218-51-10-049, Trinamic Motion Control GmbH, Waterloohain, Germany). We
used this robotic platform to impose cyclic movements of the ankle with a
fixed amplitude (70 degrees) and frequency (0.54 Hz), while continuous EES
was delivered to evoke responses in the tibialis anterior muscle (Figure 3e). EES was delivered using an
IZ2H Stimulator controlled by a RZ2 BioAmp Processor (Tucker-Davis
Technologies, Alachua, US). EES amplitude was set to approximately 1.2 times
the muscle response threshold. We tested EES frequencies ranging from 5 to
100 Hz, delivered in a random order. EMG activity of the tibialis anterior
was amplified with a PZ3 Low Impedance Amplifier (Tucker-Davis Technologies,
Alachua, US) and recorded with the RZ2 BioAmp Processor at a sampling
frequency of 24414 Hz. Ankle kinematics was record with the
Vicon motion capture system at sampling frequency of 200 Hz. For each tested
EES condition a minimum of 1 minute of recording was performed. To analyze
the modulation of the muscle responses, we used the same procedures that we
adopted in the equivalent experiment carried out in human subjects.

Formento E., Minassian K., Wagner F., Mignardot J., Le Goff C.G., Rowald A., Bloch J., Micera S., Capogrosso M, & Courtine G. (2018). Electrical spinal cord stimulation must preserve proprioception to enable locomotion in humans with spinal cord injury. Nature neuroscience, 21(12), 1728-1741.

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3

Automated Multimodal Neurological Monitoring

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Stimulus presentation, behavioral monitoring, and reward presentation were synchronized and performed automatically using a custom-written program (OpenEX and Synapse, Tucker-Davis Technologies, USA) running on a workstation (WS-8 in combination with an RZ2 bioamp processor and RZ6D multi I/O processor, Tucker-Davis Technologies, USA) and a custom-written MATLAB program running on another PC, which was connected via an A/D interface card (PCIe 6321, National Instruments) with the workstation (Fig. 1a). A monitor screen connected to the desktop PC was positioned in front of the animal’s head at a distance of 40 cm for visual stimulus presentation. Vocalizations were recorded using a microphone (MKH 8020 microphone with MZX 8000 preamplifier, Sennheiser, Germany in combination with a phantom power, PAN 48.2, Palmer) positioned 10 cm in front of the monkey’s head and connected to a multi I/O processor (RZ6D, Tucker-Davis Technologies, USA). Vocalizations were recorded using the same system at a sampling rate of 100 kHz. Vocal onset times were detected offline using software (Avisoft-SASLab Pro 5.2.13, Avisoft Bioacoustics) to ensure precise timing for data analysis. The monkey’s behavior was constantly monitored using a USB video camera (Brio, Logitech) placed in front of the monkey.

Pomberger T., Risueno-Segovia C., Gultekin Y.B., Dohmen D, & Hage S.R. (2019). Cognitive control of complex motor behavior in marmoset monkeys. Nature Communications, 10, 3796.

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4

Extracellular Recording in Face Patches

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Following fMRI localization of the relevant face patches, subjects were implanted with 64 channel NiCr microwire bundle arrays fabricated by Microprobes for extracellular recording. Monkeys SR and SP received implants in face patch AF (an overlay of functional activation in Monkey SP is shown in Figure 1A). Monkeys M and W were implanted in face patch AM (functional overlay of Monkey W is shown in Figure 1B). All recordings were conducted in a radio shielded room (ETS-Lingreen) with a RZ2 BioAmp processor (Tucker-Davis Technologies) with a 128-channel capacity collecting a broadband signal of 0.5Hz-20KHz.

Khandhadia A.P., Murphy A.P., Romanski L.M., Bizley J.K, & Leopold D.A. (2021). Audiovisual integration in macaque face patch neurons. Current Biology, 31(9), 1826-1835.e3.

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5

Simultaneous EEG-fMRI Acquisition Methodology

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The cutaneous EGG signals were recorded using an electrophysiology system (Tucker Davis Technologies, Alachua, FL, USA) while the animal was placed inside the MRI scanner during concurrent fMRI (see details in the next subsection). The electrodes were connected via Pt/Ir wires and an Omnetics 36 socket nano connector to a headstage (LP32CH-Z-32 Channel Chronic Headstage, Tucker Davis Technologies, Alachua, FL, USA) near the animal. The headstage was connected via a shielded copper cable to an amplifier (PZ5 NeuroDigitizer, Tucker Davis Technologies, Alachua, FL, USA) placed in the MRI room. The amplifier digitized the signals (sampling rate: 24 kHz; bandwidth: DC to 24 kHz; dynamic range: +/−500 mV; ADC resolution: 28 bits) and connected to a data acquisition system (RZ2 BioAmp processor, Tucker Davis Technologies, Alachua, FL, USA) outside the MRI room through an optical fiber. The data acquisition system also received and stored a TTL trigger from MRI to synchronize EGG and fMRI acquisition.

Cao J., Wang X., Chen J., Zhang N, & Liu Z. (2022). The vagus nerve mediates the stomach-brain coherence in rats. NeuroImage, 263, 119628.

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6

Cortical Mapping of Auditory Response

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After the final imaging session, we carried out extracellular electrophysiological cortical mapping experiments under anesthesia (ketamine 50 mg/kg/h + medetomidine 0.07 mg/kg/h) in each of the C57BL/6NTac.Cdh23^753A>G mice to help with the identification of primary auditory cortical areas. After removal of the glass coverslip, 64 channel (8 × 8) probes (Neuronexus, MI, USA) were inserted to record from the middle layers of auditory cortex. Electrophysiological data were acquired on a RZ2 BioAmp processor (Tucker-Davis Technologies), and collected and saved using custom-written MATLAB (MathWorks) code (https://github.com/beniamino38/benware). Stimuli were generated using a RX6 Multifunction Processor (Tucker-Davis Technologies), amplified by a TDT SA1 Stereo Amplifier (Tucker-Davis Technologies), and delivered via a modified ultrasonic dynamic loudspeaker (Vifa, Avisoft Bioacoustics, Germany) coupled to a tube that was positioned near the entrance of the mouse's left ear canal. They consisted of 200 ms pure tones spaced in one-third octave steps from 2 to 64 kHz at 40, 60 and 80 dB SPL.

Vasquez-Lopez S.A., Weissenberger Y., Lohse M., Keating P., King A.J, & Dahmen J.C. (2017). Thalamic input to auditory cortex is locally heterogeneous but globally tonotopic. eLife, 6, e25141.

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7

Simultaneous EEG-fMRI Acquisition Methodology

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The cutaneous EGG signals were recorded using an electrophysiology system (Tucker Davis Technologies, Alachua, FL, USA) while the animal was placed inside the MRI scanner during concurrent fMRI (see details in the next subsection). The electrodes were connected via Pt/Ir wires and an Omnetics 36 socket nano connector to a headstage (LP32CH-Z-32 Channel Chronic Headstage, Tucker Davis Technologies, Alachua, FL, USA) near the animal. The headstage was connected via a shielded copper cable to an amplifier (PZ5 NeuroDigitizer, Tucker Davis Technologies, Alachua, FL, USA) placed in the MRI room. The amplifier digitized the signals (sampling rate: 24 kHz; bandwidth: DC to 24 kHz; dynamic range: +/−500 mV; ADC resolution: 28 bits) and connected to a data acquisition system (RZ2 BioAmp processor, Tucker Davis Technologies, Alachua, FL, USA) outside the MRI room through an optical fiber. The data acquisition system also received and stored a TTL trigger from MRI to synchronize EGG and fMRI acquisition.

Cao J., Wang X., Chen J., Zhang N, & Liu Z. (2022). The vagus nerve mediates the stomach-brain coherence in rats. NeuroImage, 263, 119628.

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8

Neuronal Activity Recording in Primate dPul

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In 19 sessions in Monkey C and 28 sessions in Monkey L right dPul neuronal activity was recorded with up to three individually movable single platinum-tungsten (95–5%) quartz glass-insulated electrodes with impedance ranging from 1 to 1.9 MΩ for Monkey C and from 1.3 to 3.5 MΩ for Monkey L using a chamber-mounted five-channel Mini Matrix microdrive (Thomas Recording). The recording target locations were estimated similarly to the stimulation sessions using the same grids. Similar to microstimulation experiments, single custom-made stainless steel guide tubes (27 gauge) filled with the silicone oil (Thomas Recording) with a Spinocan funnel attached to the drive nozzle were used to protect electrodes during dura penetration. A reference tungsten rod or a silver wire were placed in the chamber filled with saline and were connected to the chassis of the drive. Neuronal signals were amplified (20× headstage, Thomas Recording; 5×, 128 or 32 channel PZ2 preamplifier, Tucker-Davis Technologies), digitized at 24 kHz and 16 bit resolution, and sent via fiber optics to an RZ2 BioAmp Processor (Tucker-Davis Technologies) for online filtering, display, and storage on a hard drive together with behavioral and timing data streams.

Dominguez-Vargas A.U., Schneider L., Wilke M, & Kagan I. (2017). Electrical Microstimulation of the Pulvinar Biases Saccade Choices and Reaction Times in a Time-Dependent Manner. The Journal of Neuroscience, 37(8), 2234-2257.

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9

Broadband neurophysiological data acquisition

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In this dataset, the broadband data was pre-amplified with a PZ2 Preamplifier (Tucker-Davis Technologies, Alachua, FL) with a frequency response of 3 dB: 0.35 Hz–7.5 kHz; 6 dB: 0.2 Hz–8.5 kHz^{83 (link)}. There was also an anti-aliasing filter built-in to the pre-amplifier: 4th order low-pass with a roll-off of 24 dB per octave at 7.5 kHz. It was then sampled at

F_{s} = 24414.0625

Hz and 16-bit resolution using a RZ2 BioAmp Processor (Tucker-Davis Technologies, Alachua, FL). There is an anti-aliasing filter built-in to the recording amplifier: 2nd order low-pass with a roll-off of 12 dB per octave at 7.5 kHz. As such, the recording configuration allows us to observe inter-frequency power correlations between

\sim

0.2–7500 Hz.

, & Savolainen O.W. (2021). The significance of neural inter-frequency power correlations. Scientific Reports, 11, 23190.

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10

Multimodal Neurophysiology Recording Protocol

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Electrophysiological recording and optogenetic stimulation were performed using RZ2 BioAmp processor and OpenEx software (Tucker-Davis Technologies). Silicon probes were connected through a head stage to an amplifier (Tucker-Davis Technologies; PZ5 NeuroDigitizer Amplifier) before reaching the RZ2 processor. EEGs and LFPs were filtered by 0.1–100Hz, and multi-unit activities (MUAs) were filtered by 0.3-5 kHz. Sampling rate for storage was 256Hz for LFPs, EEGs and EMGs; 25 kHz for MUAs. Spike data were collected discreetly from the same LFPs channels. Amplitude thresholds for online spike detection were set manually based on visual control. Whenever the recorded voltage exceeded a predefined threshold, a segment of 46 samples (0.48 ms before, 1.36 ms after the threshold crossing) was extracted and stored for later use. All sessions were recorded with video.

Cavelli M.L., Mao R., Findlay G., Driessen K., Bugnon T., Tononi G, & Cirelli C. (2023). Sleep/wake changes in perturbational complexity in rats and mice. iScience, 26(3), 106186.

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Rz2 bioamp processor

Lab products found in correlation

30 protocols using rz2 bioamp processor

Robotic Ankle Stimulation and EMG Recording

Robotic Ankle Stimulation and EMG Recording

Automated Multimodal Neurological Monitoring

Extracellular Recording in Face Patches

Simultaneous EEG-fMRI Acquisition Methodology

Cortical Mapping of Auditory Response

Simultaneous EEG-fMRI Acquisition Methodology

Neuronal Activity Recording in Primate dPul

Broadband neurophysiological data acquisition

Multimodal Neurophysiology Recording Protocol

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