Nisoxetine

Manufactured by Bio-Techne

Sourced in United Kingdom

Nisoxetine is a laboratory compound produced by Bio-Techne. It is a reuptake inhibitor of the neurotransmitter norepinephrine. Nisoxetine is commonly used in research applications to study the role of norepinephrine in various biological processes.

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Lab products found in correlation

Rabbit anti ha antibody clone c29f4, by Cell Signaling Technology (2 mentions) Hrp conjugated goat anti rabbit cat 111, by Jackson ImmunoResearch (2 mentions) Fluoxetine, by Bio-Techne (1 mentions) Eticlopride, by Merck Group (1 mentions) Quinpirole, by Merck Group (1 mentions) Gbr12909, by Bio-Techne (1 mentions) Mk 801, by Merck Group (1 mentions) Reboxetine, by Bio-Techne (1 mentions) 3h dopamine, by PerkinElmer (1 mentions) 3h serotonin, by PerkinElmer (1 mentions) 125i rti 55, by PerkinElmer (1 mentions) Fluvoxamine, by Bio-Techne (1 mentions) Citalopram, by Bio-Techne (1 mentions) 3h norepinephrine, by PerkinElmer (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Prism 5, by GraphPad (1 mentions)

Spelling variants of this product

Nisoxetine hydrochloride (2 citations)

6 protocols using nisoxetine

Dopamine Transporter Protein Analysis

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Nisoxetine was from Tocris-Cookson. Rabbit anti-HA antibody (clone C29F4) was from Cell Signaling Technology and rat anti-DAT antibody (MAB369) was from Millipore. HRP-conjugated goat anti-rabbit (Cat#111-035-003) and goat anti-rat (Cat#112-035-062) secondary antibodies were from Jackson ImmunoResearch. All other reagents were from either Sigma-Aldrich or Fisher Scientific and were of the highest possible grade.

Fagan R.R., Kearney P.J., Luethi D., Bolden N.C., Sitte H.H., Emery P, & Melikian H.E. (2021). Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster. Molecular psychiatry, 26(12), 7793-7802.

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Pharmacological Modulation of Locomotor Activity

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For all locomotor studies, standard Allentown cages were inserted into the locomotion chamber, and activity was measured by infrared beam breaks (Columbus Instruments). For Day/Night locomotion, mice were provided ad libitum access to food and water. For pharmacological studies, animals were removed from their home cage and placed into the locomotion chamber. Locomotor activity was measured for 90 min before injections with a single compound. The compounds tested were quinpirole (0.2 mg/kg; Sigma-Aldrich), eticlopride (0.05 mg/kg; Sigma-Aldrich), nisoxetine (10 mg/kg; Tocris Bioscience), fluoxetine (20 mg/kg; Tocris Bioscience), AMPH (2.5 mg/kg; Sigma-Aldrich), cocaine (20 mg/kg; Sigma-Aldrich), GBR-12909 (10 mg/kg; Tocris Bioscience), and MK-801 (0.2 mg/kg; Sigma-Aldrich).

Gore B.B., Miller S.M., Jo Y.S., Baird M.A., Hoon M., Sanford C.A., Hunker A., Lu W., Wong R.O, & Zweifel L.S. (2017). Roundabout receptor 2 maintains inhibitory control of the adult midbrain. eLife, 6, e23858.

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Neurotransmitter Release during Differentiation

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To detect neurotransmitter release into the media during neuronal differentiation, we collected supernatant at indicated timepoints along differentiation. The cells were treated with drugs (maprotiline 25 µM (Tocris, 0935), tomoxetine 10 µM (Tocris, 2011), nisoxetine 10 µM (Tocris, 1025) and reboxetine 10 µM (Tocris, 1982)) for 4 h or KCl (40 mM) for 30 min before collecting the medium. ELISA was performed by using the Dopamine & Noradrenaline Sensitive ELISA Assay Kit (Eagle Biosciences, BCU39-K02) following the manufacturerʼs instructions.

Tao Y., Li X., Dong Q., Kong L., Petersen A.J., Yan Y., Xu K., Zima S., Li Y., Schmidt D.K., Ayala M., Mathivanan S., Sousa A.M.M., Chang Q, & Zhang S.C. (2023). Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells. Nature biotechnology.

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Molecular Modeling of Neurotransmitter Binding

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Molecular modeling was performed using MOE
v2010 and v2011.10 software from Chemical Computing Group (Montreal,
Quebec, CA). The radioligands [³H]serotonin (∼28
Ci/mmol), [³H]dopamine (∼26 Ci/mmol), [³H]norepinephrine (∼26 Ci/mmol), and [¹²⁵I]RTI-55
(∼2200 Ci/mmol) were obtained from PerkinElmer Life and Analytical
Sciences (Foster City, CA). Nonradioactive citalopram, mazindol, nisoxetine,
and fluvoxamine were obtained from Tocris Bioscience (Ellisville,
MO). Virtual screening hit compounds were purchased from Ambinter
(Orleans, FR). C57BL/6J mice were obtained from The Jackson Laboratory
(Bar Harbor, ME). Data analysis was performed using GraphPad Prism
5.0 (GraphPad Software, San Diego, CA).

Nolan T.L., Geffert L.M., Kolber B.J., Madura J.D, & Surratt C.K. (2014). Discovery of Novel-Scaffold Monoamine Transporter Ligands via in Silico Screening with the S1 Pocket of the Serotonin Transporter. ACS Chemical Neuroscience, 5(9), 784-792.

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Immunoblotting for Dopamine Transporter

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Fagan R.R., Kearney P.J., Luethi D., Bolden N.C., Sitte H.H., Emery P., & Melikian H.E. (2021). Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.

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Norepinephrine Uptake in Spinal Cord

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Spinal cord tissue slices were preincubated in aerated (95% O 2 and 5% CO 2 ) Krebs solution for 20 min at 37 °C and subsequently incubated in a similar solution containing 5 μCi/ml radiolabelled [ 3 H]NA (10.8 Ci/mmol) (PerkinElmer, Waltham, MA, USA) for 45 min (Umeda et al., 1997) . The slices were subsequently washed 3 times with Krebs solution and placed in superfusion chambers (Vizi et al., 1985) . The chambers were perfused with aerated Krebs solution at 37 °C at a rate of 0.5 ml/min. A preperfusion period of 60 min was applied to remove all of the [ 3 H]NA isotope that was not taken up by the tissues.
In half of the uptake experiments, 1 μM nisoxetine (Tocris Bioscience, Bristol, UK) was added to the preincubation and incubation solutions and maintained throughout the experiments.

Borbély Z., Csomó B.K., Kittel Á., Gerber G., Varga G, & Vizi E.S. (2017). Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [³H]noradrenaline from a slice preparation. Brain research bulletin, 131.

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