Platinum atr

The ¹H- and ¹³C-NMR spectra were recorded on Bruker DRX-500 (500.13 and 125.76 MHz, respectively), Bruker AV 600, and Bruker AM 300 (600.13, 300.13 and 150.90 MHz, respectively) in DMSO-d₆, with TMS as internal standard. The IR spectra of solid samples were recorded on a Vertex 70 FT-IR spectrometer using a Platinum ATR (Bruker) attachment equipped with a diamond prism in a frequency range from 4000 to 400 cm⁻¹, with a resolution of 2 cm⁻¹. The results were obtained by averaging 16 scans. High-resolution mass spectra were recorded on an Agilent Technologies LCMS 6230B spectrometer (electrospray ionization). Melting points were determined on a Stuart SMP30 apparatus. Assaying of the purity of the starting materials and the synthesized compounds, as well as the analysis of reaction mixtures, was conducted by TLC on Merck TLC silica-gel 60 F₂₅₄ plates. Eluents: CHCl₃, petroleum ether, ethyl acetate, and their mixtures, in different ratios. Visualization of TLC plates was prepared under UV light or in iodine vapor. Commercially available solvents and reagents (Sigma-Aldrich, Merck, Acros Organics) were used in this work.

The chemical interaction was evaluated by Infrared spectroscopy (Alpha Bruker Platinum ATR, Germany). The surface morphology of both blank and loaded spongy composite was determined by scanning electron microscopy (SEM; JOEL; Japan). Briefly, the Sample was absorbed in liquid nitrogen, cut into cubes (3 × 3 × 1 mm) then observed at 15.0 kV^{5 (link)}. The thermal stability of the composite was evaluated by differential scanning calorimetry (DSC) using Thermo-gravimetric analysis instruments software Universal analysis (version 4.5, USA model Q600 series) and Thermo-gravimetric analysis (TGA) using TGA module of thermal analysis instrument Q5000 series; Thermal analysis system (West Sussex, UK). X-ray powder diffraction analysis was done by using XRD; model JDX 3532; Japan.

FTIR-ATR data was recorded, for the powdered bone samples, in a Bruker Optics Vertex 70 FTIR spectrometer purged by CO₂-free dry air and a Bruker Platinum ATR single reflection diamond accessory. A liquid nitrogen-cooled wide band mercury cadmium telluride (MCT) detector and a Ge on KBr substrate beamsplitter were used for the mid-IR interval (400–4000 cm⁻¹). A room temperature deuterated L-alanine-doped triglycine sulfate (DLaTGS) detector with a polyethylene window and a Si beamsplitter were used for the far-IR range (50–600 cm⁻¹).
128 scans were summed for each spectrum, at 2 cm⁻¹ resolution, applying the 3-term Blackman–Harris apodization function, yielding a wavenumber accuracy above 1 cm⁻¹. The Bruker OPUS—Spectroscopy Software (8.1 version)⁴² was used to correct the spectra regarding the wavelength dependence of the penetration depth of the electric field in ATR, for a mean refractive index of 1.25.

Functional groups of the BE sample were elucidated by FTIR spectroscopic study. A total of 1 mg powder of BE was used for FTIR. The FTIR spectrum was generated in the wave number range of 3,500–500 cm^–1 using an infrared spectrometer (Platinum ATR, ALPHA II, Bruker, Germany) at 25°C.