Institute of Cancer Research (ICR) mice (body weight, 22–25 g) were purchased from SPF Biotechnology Co., Ltd. (Beijing, China) and acclimatized 3 days before experimentation. Mice were fed in captivity under standard laboratory conditions (25 ± 2°C, 50 ± 5% RH). Mice had free access to standard rat chow pellets and water ad libitum. The experimental protocol was approved by the Institutional Animal Ethical Committee (IAEC) of Chengdu University of Traditional Chinese Medicine.
Icr mice
Manufactured by Spf Biotechnology
Sourced in China
ICR mice are a commonly used laboratory mouse strain. They are a general-purpose outbred mouse model suitable for a variety of research applications.
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10 protocols using icr mice
1
Acclimatization of ICR Mice
2
Establishing Cell and Mouse Models
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C6 glioma cells, RAW264.7 cells, bEnd.3 cells (mouse brain microvascular endothelial cells), and HUVECs (human umbilical vein endothelial cells) were supplied by the Cell Resource Center of IBMS (Beijing, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS; Gibco) and 100 IU penicillin.
Both female and male ICR mice (initially weighing 18–22 g) were purchased from SPF Biotechnology Co., Ltd. (permit number: SCXK 2019–0010, Beijing, China).
Both female and male ICR mice (initially weighing 18–22 g) were purchased from SPF Biotechnology Co., Ltd. (permit number: SCXK 2019–0010, Beijing, China).
3
Rhein Treatment in APP/PS1 Mice
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Six-month-old male APP/PS1 mice and age-matched wild-type (WT) mice with the same background (C57BL/6) were obtained from Junke Biological Co., Ltd. (Nanjing, China). Sprague-Dawley (SD) rats and ICR mice were obtained from SPF Biotechnology Co., Ltd. (Beijing, China). All rats and mice were housed with a 12-hour light/dark cycle, constant temperature (25 ± 2°C), and relative humidity of 60 ± 5%. Among them, APP/PS1 and WT mice were housed one mouse per cage. Standard chow diet and water were allowed ad libitum. All experimental procedures were approved by the Ethics Committee for Animal Care and Treatment of Beijing University of Chinese Medicine. Animal suffering and the number of animals used were minimized to the great extent.
Rhein (CAS: 478-43-3, purity > 98%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). APP/PS1 and WT mice were randomly divided into three groups: the WT group (n = 6), the APP/PS1 group (n = 6), and the APP/PS1+rhein group (n = 6). They were intravenously injected with PBS, PBS, and rhein (20 mg/kg body weight) every two days for one month, respectively. In the third week during treatment, behavior tests were performed. After one-month treatment, the mice in different groups were sacrificed for therapeutic evaluation.
Rhein (CAS: 478-43-3, purity > 98%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). APP/PS1 and WT mice were randomly divided into three groups: the WT group (n = 6), the APP/PS1 group (n = 6), and the APP/PS1+rhein group (n = 6). They were intravenously injected with PBS, PBS, and rhein (20 mg/kg body weight) every two days for one month, respectively. In the third week during treatment, behavior tests were performed. After one-month treatment, the mice in different groups were sacrificed for therapeutic evaluation.
4
Enterovirus E30 Infection in ICR Mice
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A previous study (PMID: 35420443) has demonstrated that no effective replication of E30 (strain WZ16) was found in the brain and other organs of wild-type C57 suckling mice by intracranial injecting E30 (Li et al., 2022 (link)). To better understand the effect of E30 on other types of mice, we selected E30 (strain TL1C/NM/CHN/2016) to infect Institute of Cancer Research (ICR) suckling mice, which are one of the most sensitive animals to human enterovirus. ICR mice were purchased from SPF Biotechnology Co., Ltd, (Beijing, China). Suckling mice were infected with 50 μl 106TCID50 E30 or culture medium [DEME/F12 basic and 2% fetal bovine serum (FBS)] via intramuscular injection. Weight, survival rate, and clinical scores of mice were recorded daily. The clinical disease standards were scored as follows: 0, no disease; 1, ruffled fur; 2, weight loss; 3, single limb paralysis; 4, both hind limb paralysis; and 5, moribund or dead. Mock-infected suckling mice in the E30-infected group were sacrificed 5 days after infection for brain tissue collection and transcriptome sequencing analysis.
5
Dnmt3a and Dnmt3b Heterozygous Mice Study
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All female ICR mice used in this study were obtained from SPF Biotechnology Co. Ltd. Heterozygous Dnmt3a+/− and Dnmt3b+/− mice that carry one mutant locus of Dnmt3a and Dnmt3b, respectively (23 (link)), were obtained from the NIH mutant mouse research and resource center (MMRRC, NIH). All mice were maintained in a climate-controlled room on a 12-h light/dark cycle and allowed food and water ad libitum. The China Agricultural University Institutional Animal Care and Use Committee approved this study, which was performed in accordance with the committee’s guidelines. All efforts were made to minimize animal suffering.
6
ICR Mouse Study: Standard Conditions
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This study utilized female ICR mice, Six-week-old, which were obtained from SPF Biotechnology Company (Beijing, China). The mice were housed and maintained under standard conditions (20-22°C, 50–70% humidity, 12/12-h light/dark cycle) with the food and water provided ad libitum throughout the study period. All animal care and experimental procedures were conducted in accordance with the Animal Research Committee guidelines of the Chongqing Health Center for Women and Children (The ethics committee approval number: 2022001).
7
Mouse Husbandry for In Vivo Studies
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Eight-week-old female and male ICR mice were purchased from Beijing SPF Biotechnology Corporation. All mice were healthy, not pregnant, randomly purchased, had no organic lesions and were not pretreated. All animals used were housed and bred in the Animal Facility of Sir Run Run Shaw Hospital, Zhejiang University under a standardized environment with a 12 h light/dark schedule and 20–24 °C temperature, in accordance with the institutional guidelines for laboratory animals. The animal treatment protocol was approved by the Zhejiang University Institutional Animal Care and Use Committee.
8
Mice Cancer Research Protocol
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Fifty female and fifty male SPF Institute of Cancer Research (ICR) mice (weight, 19–21 g) were purchased from SPF Biotechnology Co., Ltd. [Beijing, Certificate Number: SCXK (Jing) 2019-0010] and housed at the Tianjin Institute of Pharmaceutical Research Co., Ltd. The animal studies were performed following the recommendations for the care and use of laboratory animals of the national institutes of health. The Institutional Animal Ethical Committee approved the animal protocol of the Tianjin Institute of Pharmaceutical Research [Certificate Number: SYXK (Jin) 2016-0009, Tianjin, China].
9
Sourcing and Housing of ICR Mice
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Adult male and female ICR mice were purchased from SPF Biotechnology Co., Ltd (Beijing). All the mice were housed in the SPF animal facility at Huazhong University of Science and Technology.
10
Ethical Animal Experiments in China
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ICR mice were purchased from SPF Biotechnology Co., Ltd. (Beijing, China). All animal experiments were approved by the Ethics Review Committee of the National Institute for Viral Disease Prevention and Control, Chinese Center for Disease Control and Prevention (approval No. 20201022059).
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