V 630 uv vis

Manufactured by Jasco

Sourced in Germany, Japan, Italy

The Jasco V-630 UV-VIS is a high-performance spectrophotometer designed for a wide range of applications. It features a dual-beam optical system and a wide wavelength range from 190 to 1100 nm, allowing for accurate and reliable measurements of various samples.

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Lab products found in correlation

Erlangshen es500, by Ametek (1 mentions) Jem 2010, by Ametek (1 mentions) 1260 infinity, by Agilent Technologies (1 mentions) Kinetex xb c18 column, by Phenomenex (1 mentions) G1315d 1260 dad vl detector, by Agilent Technologies (1 mentions)

Close spelling variants of this product

V-630 (258 citations) V-630 UV-Vis spectrophotometer (57 citations) V-630 UV–vis spectrometer (6 citations) UV 630 (5 citations) V-630 Bio UV-Vis (3 citations) V-630 UV-VIS model spectrophotometer (2 citations)

10 protocols using v 630 uv vis

Spectroscopic Determination of CR Dye Stability

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In order to determine the wavelength for the value of maximum absorbance (λ_max) and its change with pH, a CR solution of 5 mg L⁻¹ was used and pH was modified from 2 to 12 using 0.1 M HCl or 0.1 M NaOH. Then, the visible absorption spectra of the solutions were recorded at room temperature with a UV–Vis spectrophotometer (V-630 UV/VIS JASCO, Japan).
To study dye stability, experiments were carried out at 25 °C by contacting 100 mL of the dye solution at the selected pH, natural pH (pH = 6) or pH = 2, and at different concentrations (5, 50 or 100 mg L⁻¹) in a thermostatic orbital water bath shaker (UNITRONIC-OR SELECTA, Spain) at a shaking rate of 100 rpm. Samples were withdrawn at predetermined times (1, 2, 6 and 7 days) and the absorbance was measured spectrophotometrically at the λ_max previously determined. The pH of dye solutions was adjusted by using 0.1 M HCl and 0.1 M NaOH solutions.

Litefti K., Freire M.S., Stitou M, & González-Álvarez J. (2019). Adsorption of an anionic dye (Congo red) from aqueous solutions by pine bark. Scientific Reports, 9, 16530.

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Ferrous Sorghum Peroxidase Binding

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All absorbance spectra and equilibrium ligand binding experiments were measured in 100 mM sodium phosphate buffer, pH 6.0, at 25.0 °C using a Jasco V630 UV–VIS spectrophotometer. Ferrous sorghum peroxidase was generated by addition of sodium dithionite to the ferric enzyme and the ferrous-CO derivative was produced by direct bubbling of CO gas through the dithionite-reduced sample. Equilibrium binding constants, K_D, were determined according to published procedures [9 (link)] and involved addition of small volumes (0.5–2.0 μL) of ligand (from an appropriate stock solution) to the protein (~2–4 μM) until no further spectral change occurred.

Nnamchi C.I., Parkin G., Efimov I., Basran J., Kwon H., Svistunenko D.A., Agirre J., Okolo B.N., Moneke A., Nwanguma B.C., Moody P.C, & Raven E.L. (2015). Structural and spectroscopic characterisation of a heme peroxidase from sorghum. Journal of Biological Inorganic Chemistry, 21, 63-70.

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Total Phenol Content Quantification

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Total phenol content (TPC) was measured by using the Folin-Ciocalteu method. 0.1 mL of the extracted solution was added to 0.5 mL of Folin-Ciocalteu solution ×10. After 15 min, the solution was mixed with 2 mL of sodium carbonate (20%) and left standing for 90 min. As for the absorbance, it was measured by a spectrophotometer (V630 UV-VIS, JASCO) at 790 nm. The gallic acid was used as a standard, and the results were expressed as mg equivalent gallic acid/g dry weight (mg GAE/g DW).

Brahimi A., El Ouardi M., Kaouachi A., Boudboud A., Hajji L., Hajjaj H, & Mazouz H. (2022). Characterization of the Biochemical Potential of Moroccan Onions (Allium cepa L.). International Journal of Food Science, 2022, 2103151.

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Optical Properties of Nanocomposite Films

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The transmittance (250–700 nm) and the opacity (600 nm) of the nanocomposite films were measured by a UV-Vis spectrophotometer (V-630 UV-Vis, Jasco, Pfungstadt, Germany). The opacity (Op) of each nanocomposite films was measured according to the following Equation (4):

O p = \frac{A b s_{600}}{x}

where Abs₆₀₀ is the absorbance measured at 600 nm and x represents the thickness of the nanocomposite films (mm). The opacity was measured in triplicate for each film and the average values and their standard deviations were determined.

Fernández-Marín R., Mujtaba M., Cansaran-Duman D., Ben Salha G., Andrés Sánchez M.Á., Labidi J, & Fernandes S.C. (2021). Effect of Deterpenated Origanum majorana L. Essential Oil on the Physicochemical and Biological Properties of Chitosan/β-Chitin Nanofibers Nanocomposite Films. Polymers, 13(9), 1507.

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Characterization of Nanoemulsion Formulation

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The nano-emulsion’s size and surface morphology images were examined via the high-resolution electron microscopy (HR-TEM) model, JOEL JEM-2010, connected with a Gatan digital camera model Erlangshen ES500 and adjusted at an acceleration voltage of 200 kV, a drop of the nanoemulsion was directly deposited on the copper coated film grid and a drop of sodium phosphotungstate (2% w/v) was then placed over the sample after drying the images was taken via the HR-TEM. The zeta potential that reflects the surface charges and the size distribution of the prepared sample was determined by dynamic light scattering using a Malvern Zetasizer model Nano ZS-90, working at 25^oC. To estimate the structural composition of the nano-emulsion, Fourier-transform infrared spectra were detected using a JASCO spectrometer in a scanning range of 4000–400 cm^-1 using KBr as a reference. The UV-Vis absorption spectra of the free drug and nanoemulsion-containing drug were recorded in the range of 800–200 nm using a UV-visible spectrophotometer (V-630 UV–vis –Jasco, Japan).

Ashosh M.I., El-Abasy M.A., El-Borady O.M., Elkhaiat I, & Ismail M.M. (2023). Comparative efficacy of difloxacin and nano-emulsion difloxacin as antibacterial agents against Salmonella enterica Serovar enteritidis in chickenss. Open Veterinary Journal, 14(1), 449-458.

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Optimization of Calcium-Based Particle Synthesis

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CA particles were prepared in DMEM as described in Section 2.2 with 0 to 8 mM of CaCl₂·2H₂O. To determine the optimum Ca²⁺ concentration, CA particles in DMB and HA particles were prepared with a wide range of Ca²⁺, 0 to 175 mM. After incubation, absorbance was immediately measured at 320 nm using a UV spectrophotometer (V-630 UV-vis, JASCO, Easton, MD, USA).

Islam R.A., Al-Busaidi H., Zaman R., Abidin S.A., Othman I, & Chowdhury E.H. (2020). Carbonate Apatite and Hydroxyapatite Formulated with Minimal Ingredients to Deliver SiRNA into Breast Cancer Cells In Vitro and In Vivo. Journal of Functional Biomaterials, 11(3), 63.

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Quantifying Polyphenols in Leonardite

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The phenolic content of leonardite was evaluated by the Folin-Ciocalteu method following Attard et al. (2013) . Polyphenolic extract was obtained by diluting 2.5 g of sample with 15 mL of methanol (100%), which was then allowed to macerate for 24 h at room temperature.
The samples were centrifugated (3000 rpm, 10 min) and the supernatants were collected and stored at -20° C for further polyphenol evaluation. The assay was performed by reacting 50 µL of extracted sample/standard with 500 µL of Folin-Ciocalteu reagent (10% in water) and 400 µL sodium carbonate (1 M). The reaction mixture was left to stand for 15 min in the dark and the total phenolic content was determined spectrophotometrically at 630 nm (JASCO V-630 UV-VIS, Germany). Calibration curves were prepared with tannic acid from 480 µg/mL to 15 µg/mL as standard. The results were expressed as mg/100g of tannic acid equivalent (mg TAEq/100g).

Dell’Anno M., Hejna M., Sotira S., Caprarulo V., Reggi S., Pilu R., Miragoli F., Callegari M.L., Panseri S., & Rossi L. (2020). Evaluation of leonardite as a feed additive on lipid metabolism and growth of weaned piglets. Animal Feed Science and Technology, 266, 114519-114519.

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Quantifying Polyphenols in Leonardite

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Dell’Anno M., Hejna M., Sotira S., Caprarulo V., Reggi S., Pilu R., Miragoli F., Callegari M.L., Panseri S, & Rossi L. (2020). Evaluation of leonardite as a feed additive on lipid metabolism and growth of weaned piglets. Animal Feed Science and Technology., 266.

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Quantifying Photosynthetic Pigments

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Chlorophyll a, b and total carotenoids were evaluated as described by Wellburn (1994) [49 (link)], using NN-dimethylformamide as solvent. Absorbance of the extract was measured at 480, 647 and 664 nm using a UV–VIS V-630 (Jasco, Milan, Italy). Results were expressed using the equations reported by Wellburn (1994) in relation to the fresh weight (FW) [49 (link)].

Malorni L., Cozzolino R., Magri A., Zampella L, & Petriccione M. (2023). Influence of Plant-Based Biostimulant (BORTAN) on Qualitative and Aromatic Traits of Rocket Salad (Diplotaxis tenuifolia L.). Plants, 12(4), 730.

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HPLC-DAD Analysis of Compound Mixtures

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The analytical HPLC system employed was Agilent 1260 Infinity, equipped with ChemStation software 3D system Rev. B 04.03, G1312C 1260 Bin Pump VL, and G1315D 1260 DAD VL detector (Agilent, Santa Clara, CA, USA), a Kinetex XB-C18 column (4.6 mm × 150 mm, 5 μm, 100 Å) (Phenomenex, Torrance, CA, USA). The Spectrophotometer: Jasco UV-VIS V-630 (Jasco Europe, Lecco, Italy). Ultrasound Bath: Elmasonic S 40 H (GEASS S.R.L., Turin, Italy). The centrifuge: Awel International MF 20-R multifunction centrifuge (MedWrench, East Point, GA, USA). Freeze-dryer: Alpha 1-4 LO plus (Martin Christ, Harz, Germany). Rotatory evaporator: IKA Rotary Evaporators RV 10 basic (IKA-Werke GmbH & Co. KG, Staufen im Breisgau, Germany).

Terenzi C., Bermudez G., Medri F., Davani L., Tumiatti V., Andrisano V., Montanari S, & De Simone A. (2024). Phenolic and Antioxidant Characterization of Fruit By-Products for Their Nutraceuticals and Dietary Supplements Valorization under a Circular Bio-Economy Approach. Antioxidants, 13(5), 604.

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