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Cytokine elisa kits

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Cytokine ELISA kits are laboratory instruments used to quantify the levels of specific cytokines in biological samples. These kits employ the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of target cytokines present in the sample.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) β nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Corning (1 mentions) Celltracker red and green, by Thermo Fisher Scientific (1 mentions) Bicinchoninic acid kit, by Bio-Rad (1 mentions) Gapdh 2118, by Cell Signaling Technology (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Sample buffer, by Bio-Rad (1 mentions) Mem per plus membrane protein extraction kit, by Thermo Fisher Scientific (1 mentions) Radio immunoprecipitation assay ripa buffer, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Lps e coli o55 b5 l2880, by Merck Group (1 mentions) Multimode microplate reader, by PerkinElmer (1 mentions) Radio immunoprecipitation assay ripa lysis buffer, by Merck Group (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ELISAs (15 citations) Mouse cytokine ELISA kits (3 citations) Enzyme-linked immunosorbent assay (ELISA) kits for cytokines (2 citations)

6 protocols using cytokine elisa kits

1

Cytokine ELISA Assay Protocol

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Lipopolysaccharides (LPS), MTT, and β-nicotinamide adenine dinucleotide phosphate were acquired from Merck (Darmstadt, Germany). BioLegend provided the cytokine ELISA kits (San Diego, CA, USA). Other high-purity chemicals were purchased commercially.
Ngernjan M., Ontawong A., Lailerd N., Mengamphan K., Sarapirom S, & Amornlerdpison D. (2022). Crocodile Oil Modulates Inflammation and Immune Responses in LPS-Stimulated RAW 264.7 Macrophages. Molecules, 27(12), 3784.
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2

Cytokine ELISA Measurement Protocol

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Cytokine ELISA kits were purchased from BioLegend (San Diego, CA) and followed the manufacturer’s protocol. Human IL-6, TNF, and MCP-1 ELISA kits (BD OptEIA) were purchased from BD Biosciences (Franklin Lakes, NJ).
Ndaw V.S., Abebayehu D., Spence A.J., Paez P.A., Kolawole E.M., Taruselli M.T., Caslin H.L., Chumanevich A.P., Paranjape A., Baker B., Barnstein B.O., Haque T.T., Kiwanuka K.N., Oskeritzian C.A, & Ryan J.J. (2017). TGFβ1 Suppresses IL-33-induced Mast Cell Function. Journal of immunology (Baltimore, Md. : 1950), 199(3), 866-873.
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3

LPS-Induced Inflammation in Macrophages

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LPS (E. Coli. O55:B5, L2880) was from Sigma-Aldrich. Anti-Rab11a (#2413), Na+/K+ ATPase (#3010) and GAPDH (#2118) Abs were from Cell Signaling. Anti-HRP–conjugated Abs were obtained from Santa Cruz Biotechnology. Anti-CD36 (ab23680, ab133625) Abs, anti-ADAM17 (ab57484, ab39163) Abs and myeloperoxidase (MPO) assay kit were ordered from Abcam. Cytokine ELISA kits were purchased from Biolegend. Rab11a and ADAM17 siRNAs were from Dharmacon. CellTracker™ Green and Red, pHrodo succinimidyl ester (pHrodo-SE), Alexa fluor 594 or 488-conjugated Abs, Lipofectamine 2000, Lipofectamine RNAiMAX transfection reagent, Mem-PER™ Plus Membrane Protein Extraction Kit, BSA, FBS, and heat-inactivated FBS were from Invitrogen. Rab11 activation assay kit was from Neweast Bioscience. Radioimmunoprecipitation assay (RIPA) buffer was from Pierce Biotechnology. Bicinchoninic acid kits and sample buffer were from Bio-Rad. DMEM/ F12 and nonenzymatic cell dissociation solution were from Cellgro. Wild type Rab11a and dominant negative mutant Rab11a S25N plasmids were a gift from Dr. Wei Guo (University of Pennsylvania, Philadelphia, PA).
Jiang C., Liu Z., Hu R., Bo L., Minshall R.D., Malik A.B, & Hu G. (2017). Inactivation of Rab11a GTPase in macrophages facilitates phagocytosis of apoptotic neutrophils. Journal of immunology (Baltimore, Md. : 1950), 198(4), 1660-1672.
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4

Quantifying Cytokine Levels via ELISA

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IL-6, IL-1β and TNF-α cytokine levels were measured by a solid-phase sandwich ELISA using commercially available cytokine ELISA kits (Biolegend, San Diego, CA, USA). The assay was performed in duplicate or triplicate, as per manufacturer’s instructions. The culture supernatants, blood plasma samples, or splenic homogenates were diluted 1:1 with coating buffer (1%BSA + 1xPBS-Tween20) and used in the assay, along with the known concentration of cytokine standards (provided with the kits) in each ELISA plate. The plates were developed and read at optical density (OD) of 450 nm in a multimode microplate reader (PerkinElmer, Waltham, MA, USA). The standard curve generated from the OD of cytokine standards was used to determine cytokine levels in the samples.
Hajam I.A., Ali F., Young J., Garcia M.A., Cannavino C., Ramchandar N, & Liu G.Y. (2021). Anti-Inflammatory Properties of Plasma from Children with Short Bowel Syndrome. Pathogens, 10(8), 1021.
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5

Cytokine and Antibody Levels Measured

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The level of IL-4, IL-5, IL-17A, and IFN-γ in BAL fluid was measured by ELISA according to the manufacturer's instructions. All cytokine ELISA kits were from BioLegend (San Diego, CA). Total and HDM-specific forms of IgE and IgG1 levels were quantified using commercial ELISA kits in serum (Southern Biotech Birmingham, Al) as described previously [41 (link)].
Movassagh H., Shan L., Duke-Cohan J.S., Chakir J., Halayko A.J., Koussih L, & Gounni A.S. (2017). Downregulation of semaphorin 3E promotes hallmarks of experimental chronic allergic asthma. Oncotarget, 8(58), 98953-98963.
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6

Investigating Inflammatory Signaling Pathways

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RPMI 1640 and penicillin-streptomycin were obtained from Gibco (Grand Island, NY, USA). LPS purified from Escherichia coli and radio-immunoprecipitation assay (RIPA) lysis buffer, protease inhibitors and β-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cytokine ELISA kits were supplied from Biolegend (USA). Antibody for PARP was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody for iNOS was obtained from Merck-Millipore (Darmstadt, Germany). Antibodies against COX-2, pp38, p38, pJNK, JNK, pERK, ERK, NF-kB and c-Jun were purchased from Cell Signaling Technology (Beverly, MA, USA). Nitrocellulose membrane was obtained from GE Healthcare (UK). Commasie Plus™ Protein Assay Reagent, ECL reagent and fetal bovine serum (FBS) were obtained from Thermo Scientific (USA).
Limtrakul P., Yodkeeree S., Pitchakarn P, & Punfa W. (2015). Suppression of Inflammatory Responses by Black Rice Extract in RAW 264.7 Macrophage Cells via Downregulation of NF-kB and AP-1 Signaling Pathways. Asian Pacific journal of cancer prevention : APJCP, 16(10).
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