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Sod activity assay kit

Manufactured by Abcam
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The SOD activity assay kit is a tool used to measure the activity of superoxide dismutase (SOD), an enzyme that plays a crucial role in cellular antioxidant defense systems. This kit provides a quantitative assessment of SOD activity in various biological samples, such as cell lysates, tissues, or body fluids.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (3 mentions) Lipid peroxidation mda assay kit, by Abcam (3 mentions) Ros assay kit, by Beyotime (2 mentions) Infinite 200 pro, by Tecan (2 mentions) Lipid peroxidation mda assay kit, by Beyotime (2 mentions) Mda assay kit, by Abcam (2 mentions) Cellular ros assay kit, by Abcam (2 mentions) Ldh assay kit, by Abcam (2 mentions) Catalase activity colorimetric fluorometric assay kit, by Abcam (1 mentions) Peroxidase activity colorimetric fluorometric assay kit, by Abcam (1 mentions) Mda assay kit, by Beyotime (1 mentions) Elisa reader, by Agilent Technologies (1 mentions) Catalase activity assay kit, by Abcam (1 mentions) Modified lowry protein assay kit, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Glutathione colorimetric assay kit, by Abcam (1 mentions) Gpx activity colorimetric assay kit, by Abcam (1 mentions) Synergy htx microplate reader, by Agilent Technologies (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Polarstar omega, by BMG Labtech (1 mentions)

Close spelling variants of this product

SOD activity kit (2 citations) Activity assay kits (2 citations) SOD kit (2 citations)

42 protocols using sod activity assay kit

1

Spermathecal Fluid Enzyme Assays

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The catalase, peroxidase, and SOD enzyme activities in the spermathecal fluid were assayed using a Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision Inc.), Peroxidase Activity Colorimetric/Fluorometric Assay Kit (BioVision Inc.), and SOD Activity Assay Kit (BioVision Inc.) according to the manufacturer’s instructions.
Park H.G., Kim B.Y., Kim J.M., Choi Y.S., Yoon H.J., Lee K.S, & Jin B.R. (2021). Upregulation of Transferrin and Major Royal Jelly Proteins in the Spermathecal Fluid of Mated Honeybee (Apis mellifera) Queens. Insects, 12(8), 690.
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2

Oxidative Stress Profiling of THP-1 Cells

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The oxidative stress status of THP-1 cells was analyzed through measuring several oxidative indicators. The levels of reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) were measured by ROS Assay Kit (Beyotime, Shanghai, China; Cat. no. S0033M), SOD Activity Assay Kit (BioVision, Milpitas, CA, USA; Cat. no. K335), MDA Assay Kit (Beyotime; Cat. no. S0131M) and iNOS kit (Abcam, Cambridge, MA, USA; Cat. no. ab253217), respectively.
Wang X, & Bai M. (2021). CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro. BMC Cardiovascular Disorders, 21, 51.
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3

Superoxide Dismutase Activity Assay

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SOD activity was analyzed using a SOD Activity Assay Kit (BioVision, Inc., Milpitas, California) according to manufacturer’s instructions [19 (link)]. The assay is a colorimetric method to measure the rate of reduction of WST-1 reagent in a water-soluble formazan dye with superoxide anion and this rate of reduction is inhibited by SOD. Briefly 104 cells were seeded in 6-well plates after treatment with fibronectin and after 24 h incubation at 37°C they were washed once and treated with irbesartan (10 and 100 μM) at 37°C for 16 h and then were put in a hypoxic chamber. Finally, SOD Kit reagents were added to cell lysates and transferred in wells of a 96-well plate and incubated for 20 min and then the absorbance was read at 450 nm. The results were used to calculate the inhibition rate and to extract the SOD activity (U/mL).
Boccellino M., Di Domenico M., Donniacuo M., Bitti G., Gritti G., Ambrosio P., Quagliuolo L, & Rinaldi B. (2018). AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress. PLoS ONE, 13(10), e0202297.
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4

Quantifying Total Superoxide Dismutase in Rat Liver

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Total superoxide dismutase (SOD; EC 1.15.1.1) activity in rat liver lysate was measured by the SOD activity assay kit (Biovision, Milpitas, CA, USA). This method uses the xanthine–xanthine oxidase system to generate superoxide anions. The superoxide anion reduces WST-1, which is converted into WST-1 formazan. The absorbance of this reduced form is recorded on an Infinite 200Pro plate reader (Tecan, Männedorf, Zürich, Switzerland). In the presence of SOD, O2− undergoes a dismutation into O2 and H2O2, thus decreasing the WST-1 formazan formation. As such, this competing assay yields to the indirect measurement of SOD activity. The SOD activity (% inhibition rate) was calculated according to the manufacturer’s instruction.
Gómez-Zorita S., González-Arceo M., Trepiana J., Aguirre L., Crujeiras A.B., Irles E., Segues N., Bujanda L, & Portillo M.P. (2020). Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding. Antioxidants, 9(11), 1042.
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5

Measuring Cellular SOD Activity

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Cells were treated with 20 µM 6-OHDA for 24 h after 1 h pre-incubation of SB. Cells were collected and lysed with lysis buffer (5 mM β-mercaptoethanol, 0.5% Triton X-100, and 0.1 mg/mL phenylmethylsulfonyl fluoride). The mixture was then centrifuged at 4 °C, 14,000 rpm for 5 min. After the adjustment of protein concentration, samples were processed for SOD determination using the SOD activity assay kit (BioVision, Exton, PA, USA). Absorbance values were obtained on an ELISA reader (Bio Tek Instruments, Inc., Winooski, VT, USA), and the SOD inhibition level was calculated as per the manufacturer’s instructions.
Feng C.W., Chen N.F., Wen Z.H., Yang W.Y., Kuo H.M., Sung P.J., Su J.H., Cheng S.Y, & Chen W.F. (2019). In Vitro and In Vivo Neuroprotective Effects of Stellettin B Through Anti-Apoptosis and the Nrf2/HO-1 Pathway. Marine Drugs, 17(6), 315.
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6

Enzymatic Activity Assay Protocol

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Microsomal cytochrome P-450 (cyt.P-450),[15 (link)] glutathione S-transferase (GST) activity[16 (link)] and uridine diphosphate glucuronyl transferase (UDPGT) activity[17 (link)] were determined using the referred methods. Catalase activity using catalase activity assay kit (BioVision, Milpitas, USA), superoxide dismutase (SOD) activity using SOD activity assay kit (BioVision, Mountain View, USA) and protein using modified Lowry protein assay kit (Thermo Scientific, Rockford, USA) were determined.
Ghosh M.K., Patra F., Ghosh S., Hossain C.M, & Mukherjee B. (2014). Antisense oligonucleotides directed against insulin-like growth factor-II messenger ribonucleic acids delay the progress of rat hepatocarcinogenesis. Journal of Carcinogenesis, 13, 2.
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7

Superoxide Dismutase Activity Assay

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The SOD activity was measured using the SOD activity assay kit (Biovision, Milpitas, CA, USA) based on the inhibition of adenochrome production by SOD during epinephrine auto-oxidation. Changes in fluorescence were read at a wavelength of 480 nm (Microplate Reader; Bio-Rad, Hercules, CA, USA).
YE S., ZHU Y., MING Y., SHE X., LIU H, & YE Q. (2014). Glycyrrhizin protects mice against renal ischemia-reperfusion injury through inhibition of apoptosis and inflammation by downregulating p38 mitogen-activated protein kinase signaling. Experimental and Therapeutic Medicine, 7(5), 1247-1252.
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8

Antioxidant Enzyme Activity Assay

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The SOD activity was detected by the SOD activity assay kit (BioVision, Milpitas, CA, USA), the cell lysate was incubated with SOD enzyme solution, and the absorbance was read at 450nm. In addition, Gpx activity was detected by the Gpx activity colorimetric assay kit (BioVision), and the absorbance was read at 340 nm. In addition, the GSH level was detected using the Glutathione Colorimetric Assay Kit (BioVision), and the cell lysate was combined with 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB) at 412 nm. We read the yellow absorbance.
Chiang M.C., Nicol C.J., Lo S.S., Hung S.W., Wang C.J, & Lin C.H. (2022). Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture. International Journal of Molecular Sciences, 23(19), 11678.
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9

Measuring Superoxide Dismutase Activity

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SOD activity was measured using the SOD Activity Assay Kit (BioVision Incorporated., CA, USA) as stated by the protocol’s instructions.
Fathy S.M., El-Dash H.A, & Said N.I. (2021). Neuroprotective effects of pomegranate (Punica granatum L.) juice and seed extract in paraquat-induced mouse model of Parkinson’s disease. BMC Complementary Medicine and Therapies, 21, 130.
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10

Colorimetric Determination of Hepatic SOD

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The hepatic SOD activity was determined by a colorimetric method using a SOD activity assay kit (Biovision). Briefly, the liver tissue was homogenized in 0.1M Tris-Cl, pH 7.4, containing 0.5% Triton X-100, 5 mM β-mercaptoethanol, and 0.1 mg/ml phenylmethylsulfonyl fluoride (PMSF) and then centrifuged at 14,000 x g for 10 min at 4°C. After a color reaction for 20 min at 37°C, the absorbance was measured at 450 nm using a spectrophotometer (Molecular Devices).
Yang D.K, & Jo D.G. (2018). Mulberry Fruit Extract Ameliorates Nonalcoholic Fatty Liver Disease (NAFLD) through Inhibition of Mitochondrial Oxidative Stress in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 8165716.
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