The contents of lactose, lactulose, and galactose were determined by HPLC using a Shimadzu LC-10A system equipped with an LC-10AD pump (Shimadzu Corporation Co., Ltd., Kyoto, Japan), an RID-6A refractive index detector (Shimadzu Corporation), and a C-R7A integrator (Shimadzu Corporation) under the following conditions: column, Polyspher CHPB column (7.9 mm φ × 300 mm; Merck KGaA, Darmstadt, Germany); guard column, Polyspher CHPB column (4 mm φ × 50 mm; Merck KGaA); column temperature, 80 °C; mobile phase, distilled water; and flow rate, 0.4 mL/min. The sample volume applied was 10 μL. Degradation products formed in the reaction were analyzed by HPLC using a JASCO LC system equipped with an 880-PU pump (JASCO Corporation Co., Ltd., Tokyo, Japan), a UV-970 ultraviolet/visible detector, an RID-6A refractive index detector (Shimadzu Corporation), and an 807-IT integrator (JASCO) under the following conditions: column, Polyspher OAKC (7.8 mm φ × 300 mm; Merck KGaA, Darmstadt, Germany); guard column, Polyspher OAKC column (4 mm φ × 50 mm; Merck KGaA); column temperature, 40 °C; mobile phase, 1 mM phosphoric acid; flow rate, 0.4 mL/min; and UV wavelength, 210 nm. The sample volume applied was 50 μL.
880 pu pump
Manufactured by Jasco
Sourced in Japan
The 880-PU pump is a laboratory equipment designed for fluid handling applications. It is a programmable pump capable of precise liquid delivery and flow control. The 880-PU features adjustable flow rates and supports a range of tubing sizes to accommodate various experimental requirements. This product is intended for use in laboratory settings by trained professionals.
Automatically generated - may contain errors
Lab products found in correlation
Lc 10ad pump, by Shimadzu (1 mentions)
Rid 6a refractive index detector, by Shimadzu (1 mentions)
C r7a integrator, by Shimadzu (1 mentions)
Polyspher chpb column, by Merck Group (1 mentions)
807 it integrator, by Jasco (1 mentions)
Lc 10a system, by Shimadzu (1 mentions)
Metacarb 67h column, by Agilent Technologies (1 mentions)
Metacarb 87h column, by Agilent Technologies (1 mentions)
4 protocols using 880 pu pump
1
HPLC Analysis of Carbohydrates in Food
2
Preparative HPLC Separation and Bioactivity Assay
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The recycling preparative HPLC system consisted of an 880-PU pump (Jasco, Tokyo, Japan), a manual injector (Model 7125, Rheodyne, Cotati, CA, USA) with a 20.0-mL sample loop and an 875-UV variable wavelength detector. The detector was set at 280 nm, 254 nm and 320 nm, respectively. The preparative column was a JAIGEL C18 reversed-phase column (250 × 30 mm, particle size 10 µm; Osaka, Japan). The mobile phase was in isocratic mode, 98% methanol. The flow rate was 10 mL/min, and the injection volume was 10 mL. The fraction highlighted in Figure S1 was collected and dried in a rotary evaporator. The dried fraction was tested against a Ganoderma culture, and the inhibitory activity was measured.
3
HPLC Quantification of Glucose and Lactate
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The quantification of glucose and lactic acid was performed by HPLC using a system that comprised of a Jasco 880-PU pump, a Jasco AS2057 Plus autosampler, a RI Jasco RI-1530 detector and a SFD 05-015 oven (Easton, USA). The chromatographic separation, was performed with a 20 min isocratic run on a MetaCarb 67H column (300 × 7.8 mm, Agilent technologies, Santa Clara, USA). Eluent was 0.005 mol•L -1 H 2 SO 4 filtered and degassed with a 0.2 μm membrane filter (GHP, Gelman, Pall, New York, USA). The flow rate was set to 0.7 mL•min -1 and the column temperature to 60 °C. The injection volume was 20 μL. L-Glucose and Llactic acid were identified by retention time (9 and 17 min, respectively) and quantified by comparing peak areas with respective calibration curves (L-glucosey = 11.634x -0.2288 (R 2 = 0.999); L-lactic acid y = 5038.8x -261.45 (R 2 = 0.999)).
4
HPLC Analysis of Sugars and Furans
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The samples were filtered through a 0.45 lm syringe filters and automatically injected (JASCO Intelligent Sampler AS 2057 Plus) through a Metacarb 87H column (300 Â 7.8 mm, Varian, USA) preheated to 60 °C by a thermostatted column compartment (Chrompack Instruments AG, Neuheim, Switzerland). The mobile phase (0.005 M H 2 SO 4 in Milli-Q water filtered through 0.2 lm Millipore Ò nylon filter and degassed) was pumped at a flow rate of 0.6 mLÁmin À1 through a JASCO 880 PU pump. Sugars and acetic acid were analyzed with a refractive index (RI) detector and furfural and hydroxymethylfurfural (HMF) with a UV detector.
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